Results 31 to 40 of about 22,843 (140)

SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis

Signal Transduction and Targeted Therapy, 2022
The coronavirus disease 2019 (COVID-19) pandemic has devastated global health. Identifying key host factors essential for SARS-CoV-2 RNA replication is expected to unravel cellular targets for the development of broad-spectrum antiviral drugs which have ...
Saisai Guo, Xiaobo Lei, Yan Chang, Jianyuan Zhao, Jing Wang, Xiaojing Dong, Qian Liu, Zixiong Zhang, Lidan Wang, Dongrong Yi, Ling Ma, Quanjie Li, Yongxin Zhang, Jiwei Ding, Chen Liang, Xiaoyu Li, Fei Guo, Jianwei Wang, Shan Cen   +18 more
doaj   +1 more source

Biochemical characterization of recombinant influenza A polymerase heterotrimer complex: Polymerase activity and mechanisms of action of nucleotide analogs. [PDF]

PLoS ONE, 2017
Influenza polymerase is a heterotrimer protein with both endonuclease and RNA-dependent RNA polymerase (RdRp) activity. It plays a critical role in viral RNA replication and transcription and has been targeted for antiviral drug development.
Ona Barauskas, Weimei Xing, Esmeralda Aguayo, Madeleine Willkom, Annapurna Sapre, Michael Clarke, Gabriel Birkus, Brian E Schultz, Roman Sakowicz, HyockJoo Kwon, Joy Y Feng   +10 more
doaj   +1 more source

Initiation of RNA Polymerization and Polymerase Encapsidation by a Small dsRNA Virus. [PDF]

PLoS Pathogens, 2016
During the replication cycle of double-stranded (ds) RNA viruses, the viral RNA-dependent RNA polymerase (RdRP) replicates and transcribes the viral genome from within the viral capsid.
Aaron M Collier, Outi L Lyytinen, Yusong R Guo, Yukimatsu Toh, Minna M Poranen, Yizhi J Tao   +5 more
doaj   +1 more source

Targeting the RdRp of Emerging RNA Viruses: The Structure-Based Drug Design Challenge

Molecules, 2020
The RNA-dependent RNA polymerase (RdRp) is an essential enzyme for the viral replication process, catalyzing the viral RNA synthesis using a metal ion-dependent mechanism.
Francesca Picarazzi, Ilaria Vicenti, Francesco Saladini, Maurizio Zazzi, Mattia Mori   +4 more
doaj   +1 more source

Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for genome replication

Communications Biology, 2022
Loundras et al. report on the fibril components of the RNA-dependent RNA polymerase RdRp from foot-and-mouth disease virus. They demonstrate that higher-order fibril-based interactions create multiple complex structures within which RNA replication can ...
Eleni-Anna Loundras, James Streetley, Morgan R. Herod, Rebecca Thompson, Mark Harris, David Bhella, Nicola J. Stonehouse   +6 more
doaj   +1 more source

Identification of a Conserved RNA-dependent RNA Polymerase (RdRp)-RNA Interface Required for Flaviviral Replication [PDF]

Journal of Biological Chemistry, 2016
Dengue virus, an ∼10.7-kb positive-sense RNA virus, is the most common arthropod-communicated pathogen in the world. Despite dengue's clear epidemiological importance, mechanisms for its replication remain elusive. Here, we probed the entire dengue genome for interactions with viral RNA-dependent RNA polymerase (RdRp), and we identified the dominant ...
Kenneth, Hodge, Chairat, Tunghirun, Maliwan, Kamkaew, Thawornchai, Limjindaporn, Pa-Thai, Yenchitsomanus, Sarin, Chimnaronk   +5 more
openaire   +2 more sources

Signatures of Nucleotide Analog Incorporation by an RNA-Dependent RNA Polymerase Revealed Using High-Throughput Magnetic Tweezers

Cell Reports, 2017
Summary: RNA viruses pose a threat to public health that is exacerbated by the dearth of antiviral therapeutics. The RNA-dependent RNA polymerase (RdRp) holds promise as a broad-spectrum, therapeutic target because of the conserved nature of the ...
David Dulin, Jamie J. Arnold, Theo van Laar, Hyung-Suk Oh, Cheri Lee, Angela L. Perkins, Daniel A. Harki, Martin Depken, Craig E. Cameron, Nynke H. Dekker   +9 more
doaj   +1 more source

Regulation of viral RNA-dependent RNA polymerases by phosphorylation

Frontiers in Virology, 2023
RNA viruses encode an RNA-dependent RNA polymerase (RdRp), which is essential for transcription and replication of their genome since host cells lack equivalent enzymes.
Camille Duflos, Thomas Michiels
doaj   +1 more source

Assessment of nucleotide/nucleoside analog intervention in primer-dependent viral RNA-dependent RNA polymerases

STAR Protocols, 2022
Summary: Nucleotide/nucleoside analogs (NAs) are important compounds used in antiviral drug development. To understand the action mode of NA drugs, we present an enzymology protocol to initially evaluate the intervention mechanism of the NTP forms of NAs
Qiaojie Liu, Jiqin Wu, Peng Gong
doaj   +1 more source

RNA-dependent RNA Polymerase Assay for Hepatitis E Virus

Bio-Protocol, 2017
RNA-dependent RNA polymerase (RdRp) is essential for the replication of viral RNA for RNA viruses. It synthesizes the complementary strand of viral genomic RNA, which is used subsequently as a template to generate more copies of viral genome.
Vidya Nair, Saumya Anang, Akriti Srivastava, Milan Surjit   +3 more
doaj   +1 more source