Results 41 to 50 of about 3,122,867 (388)

Identification of RNA-binding Proteins

Bio-Protocol, 2016
This protocol describes the extraction of RNA-binding proteins (RBPs) from cell lysates. In order to pull down target RBPs, 5-bromo-UTP (BrUTP)-incorporated RNA probes are used, which are generated by in vitro transcription.
Kazuya Masuda, Tadamitsu Kishimoto
doaj   +1 more source

PTRE-seq reveals mechanism and interactions of RNA binding proteins and miRNAs [PDF]

, 2018
A large number of RNA binding proteins (RBPs) and miRNAs bind to the 3′ untranslated regions of mRNA, but methods to dissect their function and interactions are lacking.
Chaudhari, Hemangi G, Cohen, Barak A, Cottrell, Kyle A, Djuranovic, Sergej   +3 more
core   +3 more sources

The IGF2BP family of RNA binding proteins links epitranscriptomics to cancer.

Seminars in Cancer Biology, 2022
RNA binding proteins that act at the post-transcriptional level display a richness of mechanisms to modulate the transcriptional output and respond to changing cellular conditions. The family of IGF2BP proteins recognize mRNAs modified by methylation and
Deepthi Ramesh-Kumar, S. Guil
semanticscholar   +1 more source

γ-AApeptides bind to RNA by mimicking RNA-binding proteins [PDF]

Organic & Biomolecular Chemistry, 2011
The interactions between proteins and RNAs are of vital importance for many cellular processes, including transcription and processing of RNA, translation, and viral infections. Here we report an γ-AApeptide that can mimic HIV-1 Tat protein and bind to TAR RNAs of HIV and BIV with nanomolar affinity, comparable to that of the RNA-binding fragment of ...
Youhong, Niu, Alisha Jonesy, Jones, Haifan, Wu, Gabriele, Varani, Jianfeng, Cai   +4 more
openaire   +2 more sources

Stress granules, RNA-binding proteins and polyglutamine diseases: too much aggregation?

Cell Death and Disease, 2021
Stress granules (SGs) are membraneless cell compartments formed in response to different stress stimuli, wherein translation factors, mRNAs, RNA-binding proteins (RBPs) and other proteins coalesce together.
Adriana Marcelo, Rebekah Koppenol, L. P. de Almeida, Carlos A. Matos, Clévio Nóbrega   +4 more
semanticscholar   +1 more source

Protein interactions in Xenopus germ plasm RNP particles [PDF]

, 2013
Hermes is an RNA-binding protein that we have previously reported to be found in the ribonucleoprotein (RNP) particles of Xenopus germ plasm, where it is associated with various RNAs, including that encoding the germ line determinant Nanos1.
A Anyanful, A Mir, AM Hulstrand, AT Hertig, Barbara Bardoni, C Hudson, C Yost, CA Schneider, CD Hu, D Jin, DL Gard, F Bontems, F Lai, FF Lai, FL Marlow, GT Morgan, Hugh R. Woodland, HW Song, HY Li, I Yanai, J Heasman, JB Bowes, JK Joung, JK Yisraeli, K Kosaka, KL Poon, LA Berekelya, M Claussen, M Kloc, M Kloc, ML King, NP Torpey, P Anderson, RJ Machado, RS Hames, S Miyamoto, S Nijjar, Sarbjit Nijjar, SM Bilinski, T Fukuda, TK Kerppola, WV Gerber, WV Gerber, Y Saka, Y Zhou, ZF Nizami   +45 more
core   +3 more sources

Robust single-cell discovery of RNA targets of RNA binding proteins and ribosomes

Nature Methods, 2021
RNA-binding proteins (RBPs) are critical regulators of gene expression and RNA processing that are required for gene function. Yet the dynamics of RBP regulation in single cells is unknown.
Kristopher W Brannan, I. A. Chaim, Ryan J. Marina, Brian A. Yee, Eric Kofman, Daniel A. Lorenz, Pratibha Jagannatha, Kevin D. Dong, Assael A. Madrigal, J. Underwood, Gene W. Yeo   +10 more
semanticscholar   +1 more source

Saturation mutagenesis reveals manifold determinants of exon definition. [PDF]

, 2017
To illuminate the extent and roles of exonic sequences in the splicing of human RNA transcripts, we conducted saturation mutagenesis of a 51-nt internal exon in a three-exon minigene.
Anquetil, Vincent, Arias, Mauricio A., Chasin, Lawrence A., Ju, Jingyue, Kalachikov, Sergey, Ke, Shengdong, Maity, Alisha, Russo, James J, Yang, Anthony, Zamalloa, Jorge Rojas   +9 more
core   +2 more sources

RNA-Binding Proteins Tia-1 and Tiar Link the Phosphorylation of Eif-2α to the Assembly of Mammalian Stress Granules

Journal of Cell Biology, 1999
In response to environmental stress, the related RNA-binding proteins TIA-1 and TIAR colocalize with poly(A)+ RNA at cytoplasmic foci that resemble the stress granules (SGs) that harbor untranslated mRNAs in heat shocked plant cells (Nover et al.
N. Kedersha, M. Gupta, Wei Li, Ira Miller, P. Anderson   +4 more
semanticscholar   +1 more source

Chloroplast RNA-binding proteins [PDF]

Current Genetics, 2003
Chloroplast gene expression is regulated by nucleus-encoded factors, which mainly act at the post-transcriptional level. Plastid RNA-binding proteins (RBPs) represent good candidates for mediating these functions. The picture emerging from recent analyses is that of a great number of differentially regulated RBPs, which are organized in distinct ...
openaire   +2 more sources