Results 51 to 60 of about 4,565,908 (262)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba [PDF]

, 2010
Archaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro, and we show in the present study that it binds more tightly ...
Petrovic-Stojanovska, Biljana, White, Malcolm F, Jelinska, Clare, Ingledew, W John, Ingledew, William John, White, Malcolm F; id_orcid   +5 more
core   +1 more source

Functional Advantages of Conserved Intrinsic Disorder in RNA-Binding Proteins. [PDF]

PLoS ONE, 2015
Proteins form large macromolecular assemblies with RNA that govern essential molecular processes. RNA-binding proteins have often been associated with conformational flexibility, yet the extent and functional implications of their intrinsic disorder have
Mihaly Varadi, Fruzsina Zsolyomi, Mainak Guharoy, Peter Tompa   +3 more
doaj   +1 more source

Identification of Intrinsic RNA Binding Specificity of Purified Proteins by in vitro RNA Immunoprecipitation (vitRIP)

Bio-Protocol, 2021
RNA-protein interactions are often mediated by dedicated canonical RNA binding domains. However, interactions through non-canonical domains with unknown specificity are increasingly observed, raising the question how RNA targets are recognized. Knowledge
Marisa Müller , Tamas Schauer, Peter Becker   +2 more
doaj   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

The cold-inducible RNA-binding motif protein 3 (RBM3) prevents cell death through various mechanisms [PDF]

, 2016
Moderate hypothermia promotes the survival of neural cells in a wide range of brain disorders. During hypothermia, a subgroup of proteins including two homologues, RNA-binding motif protein 3 (RBM3) and cold-inducible RNA-binding protein (CIRP), is ...
Zhu, Xinzhou
core   +1 more source

Comprehensive landscape of the functions and prognostic value of RNA binding proteins in uterine corpus endometrial carcinoma

Frontiers in Molecular Biosciences, 2022
Background: The dysregulation of RNA binding proteins (RBPs) is involved in tumorigenesis and progression. However, information on the overall function of RNA binding proteins in Uterine Corpus Endometrial Carcinoma (UCEC) remains to be studied.
Yong Yao, Yong Yao, Yong Yao, Kangping Liu, Kangping Liu, Kangping Liu, Yuxuan Wu, Yuxuan Wu, Yuxuan Wu, Jieyu Zhou, Jieyu Zhou, Jieyu Zhou, Heyue Jin, Heyue Jin, Heyue Jin, Yimin Zhang, Yimin Zhang, Yimin Zhang, Yumin Zhu, Yumin Zhu, Yumin Zhu   +20 more
doaj   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source

Post-translational Modifications And Rna-binding Proteins

, 2017
RNA-binding proteins affect cellular metabolic programs through development and in response to cellular stimuli. Though much work has been done to elucidate the roles of a handful of RNA-binding proteins and their effect on RNA metabolism, the progress ...
Mario H.; Massirer, Michael T.; Bengtson, Lovci, Katlin B.   +3 more
core   +1 more source