Captivating Perplexities of Spinareovirinae 5′ RNA Caps [PDF]
Viruses, 2021 RNAs with methylated cap structures are present throughout multiple domains of life. Given that cap structures play a myriad of important roles beyond translation, such as stability and immune recognition, it is not surprising that viruses have adopted ...
Justine Kniert +2 more
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Dinucleoside polyphosphates act as 5′-RNA caps in bacteria [PDF]
Nature Communications, 2020 Nicotinamide adenine dinucleotide and coenzyme A serve as a 5′-cap of prokaryotic RNA. Here the authors report that methylated and non-methylated dinucleoside polyphosphates (Np n Ns) exist as Escherichia coli RNA caps which can be cleaved by 5 ...
Oldřich Hudeček +9 more
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NADcapPro and circNC: methods for accurate profiling of NAD and non-canonical RNA caps in eukaryotes [PDF]
Communications Biology, 2023 Accurate identification of NAD-capped RNAs is essential for delineating their generation and biological function. Previous transcriptome-wide methods used to classify NAD-capped RNAs in eukaryotes contain inherent limitations that have hindered the ...
Sunny Sharma +4 more
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Synthesis of 5′-Thiamine-Capped RNA [PDF]
Molecules, 2020 RNA 5′-modifications are known to extend the functional spectrum of ribonucleotides. In recent years, numerous non-canonical 5′-modifications, including adenosine-containing cofactors from the group of B vitamins, have been confirmed in all kingdoms of ...
Marvin Möhler +2 more
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Pan-cancer multi-omics characterization of calcyphosine and its revealed links to the immune microenvironment and regulatory networks in endometrial carcinoma [PDF]
Frontiers in ImmunologyObjectiveThe calcium-dependent activator protein for secretion (CAPS) has emerged as a protein of interest in tumor biology; however, its functional significance in endometrial carcinoma (EC) remains largely unexplored.MethodsWe performed an integrative ...
Xi Chen +7 more
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mRNA caps accumulate in stress granules and are essential for their formation [PDF]
Cellular and Molecular Life SciencesStress granules (SGs) are essential cytoplasmic, membraneless organelles that form in response to cellular stress, functioning to prevent mRNA translation and protect mRNA from damage. However, the mechanism of SG formation remains largely unknown. Here,
Mingxin Guo +7 more
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RNA Cap Methyltransferase Activity Assay
Bio-Protocol, 2018 Methyltransferases that methylate the guanine-N7 position of the mRNA 5’ cap structure are ubiquitous among eukaryotes and commonly encoded by viruses. Here we provide a detailed protocol for the biochemical analysis of RNA cap methyltransferase activity
Jackson Trotman, Daniel Schoenberg
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Immunoprecipitation of Tri-methylated Capped RNA
Bio-Protocol, 2018 Cellular quiescence (also known as G0 arrest) is characterized by reduced DNA replication, increased autophagy, and increased expression of cyclin-dependent kinase p27Kip1.
Karen Hayes +4 more
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If the 5’ cap fits (wear it) – Non-canonical RNA capping
RNA BiologyRNA capping is a prominent RNA modification that influences RNA stability, metabolism, and function. While it was long limited to the study of the most abundant eukaryotic canonical m7G cap, the field recently went through a large paradigm shift with the
Jiří František Potužník +1 more
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CompasSeq: epitranscriptome-wide percentage assessment of metabolite-capped RNA at the transcript resolution [PDF]
Nature CommunicationsNucleotide-containing metabolites, e.g., NAD, can serve as noncanonical initiating nucleotides (NCIN) during transcription, yielding NCIN-capped RNAs (NCIN-RNAs).
Yuanbo Liu +6 more
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