Results 11 to 20 of about 29,994 (285)
If the 5’ cap fits (wear it) – Non-canonical RNA capping
RNA BiologyRNA capping is a prominent RNA modification that influences RNA stability, metabolism, and function. While it was long limited to the study of the most abundant eukaryotic canonical m7G cap, the field recently went through a large paradigm shift with the
Jiří František Potužník +1 more
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Synthesis of 5′-Thiamine-Capped RNA [PDF]
Molecules, 2020 RNA 5′-modifications are known to extend the functional spectrum of ribonucleotides. In recent years, numerous non-canonical 5′-modifications, including adenosine-containing cofactors from the group of B vitamins, have been confirmed in all kingdoms of ...
Marvin Möhler +2 more
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Immunoprecipitation of Tri-methylated Capped RNA
Bio-Protocol, 2018 Cellular quiescence (also known as G0 arrest) is characterized by reduced DNA replication, increased autophagy, and increased expression of cyclin-dependent kinase p27Kip1.
Karen Hayes +4 more
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RNA Cap Methyltransferase Activity Assay
Bio-Protocol, 2018 Methyltransferases that methylate the guanine-N7 position of the mRNA 5’ cap structure are ubiquitous among eukaryotes and commonly encoded by viruses. Here we provide a detailed protocol for the biochemical analysis of RNA cap methyltransferase activity
Jackson Trotman, Daniel Schoenberg
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Staphylococcus aureus Small RNAs Possess Dephospho-CoA 5′-Caps, but No CoAlation Marks
Non-Coding RNA, 2022 Novel features of coenzyme A (CoA) and its precursor, 3′-dephospho-CoA (dpCoA), recently became evident. dpCoA was found to attach to 5′-ends of small ribonucleic acids (dpCoA-RNAs) in two bacterial species (Escherichia coli and Streptomyces venezuelae).
Christian Löcherer +3 more
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RNA capping by mitochondrial and multi-subunit RNA polymerases [PDF]
Transcription, 2018 Recently, it was found that bacterial and eukaryotic transcripts are capped with cellular cofactors installed by their respective RNA polymerases (RNAPs) during transcription initiation. We now show that mitochondrial RNAP efficiently caps transcripts with ADP - containing cofactors.
Christina Julius +2 more
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The mechanism of RNA capping by SARS-CoV-2 [PDF]
Nature, 2022 Abstract The SARS-CoV-2 RNA genome contains a 5′-cap that facilitates translation of viral proteins, protection from exonucleases and evasion of the host immune response1-4. How this cap is made is not completely understood. Here, we reconstitute the SARS-CoV-2 7MeGpppA2′-O-Me-RNA cap using virally encoded non-structural proteins (nsps).
Gina J. Park +11 more
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CK2 phosphorylation of CMTR1 promotes RNA cap formation and influenza virus infection [PDF]
Cell ReportsSummary: The RNA cap methyltransferase CMTR1 methylates the first transcribed nucleotide of RNA polymerase II transcripts, impacting gene expression mechanisms, including during innate immune responses.
Radoslaw Lukoszek +8 more
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Enzymatic synthesis of RNAs capped with nucleotide analogues reveals the molecular basis for substrate selectivity of RNA capping enzyme: impacts on RNA metabolism. [PDF]
PLoS ONE, 2013 RNA cap binding proteins have evolved to specifically bind to the N7-methyl guanosine cap structure found at the 5' ends of eukaryotic mRNAs. The specificity of RNA capping enzymes towards GTP for the synthesis of this structure is therefore crucial for ...
Moheshwarnath Issur +3 more
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Enzymology of RNA cap synthesis [PDF]
WIREs RNA, 2010 AbstractThe 5′ guanine‐N7 methyl cap is unique to cellular and viral messenger RNA (mRNA) and is the first co‐transcriptional modification of mRNA. The mRNA cap plays a pivotal role in mRNA biogenesis and stability, and is essential for efficient splicing, mRNA export, and translation.
Agnidipta, Ghosh, Christopher D, Lima
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