Results 11 to 20 of about 28,766 (215)

Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA [PDF]

Nucleic Acids Research, 2019
Abstract Chemical modification of transcripts with 5′ caps occurs in all organisms. Here, we report a systems-level mass spectrometry-based technique, CapQuant, for quantitative analysis of an organism's cap epitranscriptome. The method was piloted with 21 canonical caps—m7GpppN, m7GpppNm, GpppN, GpppNm, and m2,2,7GpppG—and 5 ...
Jin Wang, Bing Liang Alvin Chew, Yong Lai, Hongping Dong, Luang Xu, Seetharamsingh Balamkundu, Weiling Maggie Cai, Liang Cui, Chuan Fa Liu, Xin-Yuan Fu, Zhenguo Lin, Pei-Yong Shi, Timothy K. Lu, Dahai Luo, Samie R. Jaffrey, Peter C. Dedon   +15 more
openaire   +6 more sources

White and brown adipose tissue share a convergent fibro-adipogenic progenitor population [PDF]

EMBO Reports
Adipose tissue heterogeneity has emerged as a central factor in regulating adipose tissue function in physiology and pathophysiology, yet tools to model and study this diversity in vitro remain limited.
Hoang V Bui, Julia K Hansen, Valentina Lo Sardo, Andrea Galmozzi   +3 more
doaj   +2 more sources

Interplay between bacterial 5′-NAD-RNA decapping hydrolase NudC and DEAD-box RNA helicase CsdA in stress responses

mSystems, 2023
Both prokaryotic and eukaryotic RNAs can be 5′-capped by the metabolite nicotinamide adenine dinucleotide (NAD). Nudix hydrolases, such as bacterial NudC, specifically remove NAD-caps; however, the molecular and cellular functions of these ...
Milda Mickutė, Renatas Krasauskas, Kotryna Kvederavičiūtė, Gytė Tupikaitė, Aleksandr Osipenko, Algirdas Kaupinis, Monika Jazdauskaitė, Raminta Mineikaitė, Mindaugas Valius, Viktoras Masevičius, Giedrius Vilkaitis   +10 more
doaj   +1 more source

Enzymology of RNA cap synthesis [PDF]

WIREs RNA, 2010
AbstractThe 5′ guanine‐N7 methyl cap is unique to cellular and viral messenger RNA (mRNA) and is the first co‐transcriptional modification of mRNA. The mRNA cap plays a pivotal role in mRNA biogenesis and stability, and is essential for efficient splicing, mRNA export, and translation.
Agnidipta, Ghosh, Christopher D, Lima
openaire   +2 more sources

Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins

Viruses, 2022
An extensive understanding of the interactions between host cellular and viral proteins provides clues for studying novel antiviral strategies. Porcine circovirus type 3 (PCV3) and type 4 (PCV4) have recently been identified as viruses that can ...
Jianwei Zhou, Yongxia Wang, Linyi Zhou, Yonghui Qiu, Jie Zhao, Beining Dai, Xufei Feng, Lei Hou, Jue Liu   +8 more
doaj   +1 more source

Disparate Roles of Oxidative Stress in Rostral Ventrolateral Medulla in Age-Dependent Susceptibility to Hypertension Induced by Systemic l-NAME Treatment in Rats

Biomedicines, 2022
This study aims to investigate whether tissue oxidative stress in the rostral ventrolateral medulla (RVLM), where sympathetic premotor neurons reside, plays an active role in age-dependent susceptibility to hypertension in response to nitric oxide (NO ...
Yung-Mei Chao, Hana Rauchová, Julie Y. H. Chan   +2 more
doaj   +1 more source

The expanding field of non-canonical RNA capping: new enzymes and mechanisms

Royal Society Open Science, 2021
Recent years witnessed the discovery of ubiquitous and diverse 5′-end RNA cap-like modifications in prokaryotes as well as in eukaryotes. These non-canonical caps include metabolic cofactors, such as NAD+/NADH, FAD, cell wall precursors UDP-GlcNAc ...
Jana Wiedermannová, Christina Julius, Yulia Yuzenkova   +2 more
doaj   +1 more source

Preparation of RNAs with non-canonical 5′ ends using novel di- and trinucleotide reagents for co-transcriptional capping

Frontiers in Molecular Biosciences, 2022
Many eukaryotic and some bacterial RNAs are modified at the 5′ end by the addition of cap structures. In addition to the classic 7-methylguanosine 5′ cap in eukaryotic mRNA, several non-canonical caps have recently been identified, including NAD-linked ...
Anaïs Depaix, Anaïs Depaix, Ewa Grudzien-Nogalska, Bartlomiej Fedorczyk, Megerditch Kiledjian, Jacek Jemielity, Joanna Kowalska   +6 more
doaj   +1 more source

CapZyme-Seq: A 5′-RNA-Seq Method for Differential Detection and Quantitation of NAD-Capped and Uncapped 5′-Triphosphate RNA

STAR Protocols, 2020
Summary: Nucleoside-containing metabolites such as the oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD+ and NADH), 3′-desphospho-coenzyme A (dpCoA), and flavin adenine dinucleotide (FAD) can be incorporated as RNA 5′ end caps by ...
Irina O. Vvedenskaya, Bryce E. Nickels
doaj   +1 more source

Cultivar-specific markers, mutations, and chimerisim of Cavendish banana somaclonal variants resistant to Fusarium oxysporum f. sp. cubense tropical race 4

BMC Genomics, 2022
Background The selection of tissue culture–derived somaclonal variants of Giant Cavendish banana (Musa spp., Cavendish sub-group AAA) by the Taiwan Banana Research Institute (TBRI) has resulted in several cultivars resistant to Fusarium oxysporum f.
Bo-Han Hou, Yi-Heng Tsai, Ming-Hau Chiang, Shu-Ming Tsao, Shih-Hung Huang, Chih-Ping Chao, Ho-Ming Chen   +6 more
doaj   +1 more source