Results 91 to 100 of about 37,396 (213)

The evaluation of an identification algorithm for Mycobacterium species using the 16S rRNA coding gene and rpoB

International Journal of Mycobacteriology, 2012
Background: Conventional biochemical tests are the standard for the identification of Mycobacterium species, but molecular identifications are becoming more prevalent.
Yuko Kazumi, Satoshi Mitarai
doaj   +1 more source

PartialrpoBgene sequencing for identification ofLeptospiraspecies [PDF]

FEMS Microbiology Letters, 2006
The usual target for sequence-based identification of Leptospira species is the 16S rRNA gene. However, because the 16S rRNA gene is not polymorphic enough, it is necessary to sequence a 1500 bp segment of this gene for accurate identification. Based on the alignment of previously determined rpoB of three Leptospira strains, we designed and tested a ...
Bernard, La Scola, Lan T M, Bui, Guy, Baranton, Atieh, Khamis, Didier, Raoult   +4 more
openaire   +2 more sources

Nested PCR to optimize <i>rpoB</i> metabarcoding for low-concentration and host-associated bacterial DNA. [PDF]

Microbiol Spectr
Housekeeping genes have proven to be effective taxonomic markers for characterizing bacterial microbiota in short-read amplicon metabarcoding studies. A region of the rpoB gene has been shown to minimize Operational Taxonomic Unit (OTU) overestimation ...
Leclerc L, Agoutin G, Brévault T, Champion A, Mainguy J, Nègre N, Yainna S, Pascal G, Gaudriault S, Ogier J-C.   +9 more
europepmc   +2 more sources

Mutations in 1700 Bp Fragment of Rpob Gene of Multi-drug Resistant Mycobacterium Tuberculosis Isolate [PDF]

, 2012
This research aimed to amplify a 1700 bp fragment of rpoB gene of multidrug resistance M. tuberculosis (MDR-TB) isolate and determine types of mutation beyond the core region (hot-spot region).
Junitha, I. K. (I), Mantik-Astawa, I. N. (I), Sukardika, I. K. (I), Yowani, C. (Chandra)   +3 more
core  

Prevalence of non-aureus Staphylococcus species causing intramammary infections in Canadian dairy herds [PDF]

, 2017
Non-aureus staphylococci (NAS), the microorganisms most frequently isolated from bovine milk worldwide, are a heterogeneous group of numerous species. To establish their importance as a group, the distribution of individual species needs to be determined.
Barkema, Herman W., Carson, Domonique A., Condas, Larissa A.Z., De Buck, Jeroen, De Vliegher, Sarne, Dufour, Simon, Kastelic, John P., Middleton, John R., Naushad, Sohail, Nobrega, Diego B., Zadoks, Ruth N.   +10 more
core   +1 more source

Techniques to Detect and Quantify the Bacterial Metalloprotease AprX in Bovine Milk: A Review

Comprehensive Reviews in Food Science and Food Safety, Volume 25, Issue 2, March 2026.
ABSTRACT The heat‐stable metalloprotease AprX, secreted by psychrotrophic Pseudomonas spp., is a major cause of quality deterioration in dairy products, particularly ultrahigh temperature (UHT) milk. This review synthesizes the evolution and current state of detection and quantification techniques for AprX in bovine milk, covering traditional ...
Aritra Sinha, Alan L. Kelly
wiley   +1 more source

The complete 12 Mb genome and transcriptome of Nonomuraea gerenzanensis with new insights into its duplicated “magic” RNA polymerase

Scientific Reports, 2016
In contrast to the widely accepted consensus of the existence of a single RNA polymerase in bacteria, several actinomycetes have been recently shown to possess two forms of RNA polymerases due the to co-existence of two rpoB paralogs in their genome ...
Valeria D’Argenio, Mauro Petrillo, Daniela Pasanisi, Caterina Pagliarulo, Roberta Colicchio, Adelfia Talà, Maria Stella de Biase, Mario Zanfardino, Emanuela Scolamiero, Chiara Pagliuca, Antonio Gaballo, Annunziata Gaetana Cicatiello, Piergiuseppe Cantiello, Irene Postiglione, Barbara Naso, Angelo Boccia, Miriana Durante, Luca Cozzuto, Paola Salvatore, Giovanni Paolella, Francesco Salvatore, Pietro Alifano   +21 more
doaj   +1 more source

rpoB sequence analysis as a novel basis for bacterial identification [PDF]

Molecular Microbiology, 1997
Comparison of the sequences of conserved genes, most commonly those encoding 16S rRNA, is used for bacterial genotypic identification. Among some taxa, such as the Enterobacteriaceae, variation within this gene does not allow confident species identification.
C, Mollet, M, Drancourt, D, Raoult
openaire   +2 more sources

Amplication of 0.7kb Fragment Katg Gene From Clinical Multi Drug Resistant Tuberculosis Isolate in Bali [PDF]

, 2013
During last decade has seen a particular increase in the occurrence of drug-resistant of tuberculosis (DR-TB) and multi-DR strains, such as Isoniazid (INH) resistant strains of M. tuberculosis. INH resistance is more frequently associated with mutations
Dwiputri, A. W. (A), Ratnayani, K. (K), Yowani, A. S. (and)   +2 more
core  

Erwinia oleae sp. nov., isolated from olive knots caused by Pseudomonas savastanoi pv. savastanoi [PDF]

, 2011
Three endophytic bacterial isolates were obtained in Italy from olive knots caused by Pseudomonas savastanoi pv. savastanoi. Phenotypic tests in combination with 16S rRNA gene sequence analysis indicated a phylogenetic position of these isolates in the ...
Brady, Carrie, Buonaurio, Roberto, Cleenwerck, Ilse, De Vos, Paul, Hosni, Taha, Moretti, Chiaraluce, Vandemeulebroecke, Katrien   +6 more
core   +1 more source