Results 11 to 20 of about 28,118 (222)

Detection of Mycobacterium tuberculosis in extrapulmonary biopsy samples using PCR targeting IS6110, rpoB and nested-rpoB PCR Cloning [PDF]

Frontiers in Microbiology, 2015
Present study was aimed to examine the diagnostic utility of PCR and nested PCR techniques for the detection of Mycobacterium tuberculosis (MTB) DNA in samples from patients with extra pulmonary tuberculosis (EPTB).
Hossein eMeghdadi, Azar Dokht eKhosravi, Ata eGhadiri, Ameneh eAlami, Amir Hossein eSina   +4 more
doaj   +5 more sources

Borderline rpoB mutations transmit at the same rate as common rpoB mutations in a tuberculosis cohort in Bangladesh [PDF]

Microbial Genomics, 2023
The spread of multidrug-resistant tuberculosis (MDR-TB) is a growing problem in many countries worldwide. Resistance to one of the primary first-line drugs, rifampicin, is caused by mutations in the Mycobacterium tuberculosis rpoB gene.
Rigouts, Leen, Lempens, Pauline, Decroo, T., Hossain, M.A., van Deun, A., Behruznia, Mabi; id_orcid, de Jong, B.C., Meehan, Conor J, Aung, K., Decroo, Tom, Behruznia, Mahboobeh, Meehan, Conor J., Hossain, Mohammad A., Van Deun, Armand, de Jong, Bouke C., Aung, Kya J.M., Aung, Kya J. M.   +16 more
core   +5 more sources

Phylogeny of the family Pasteurellaceae based on rpoB sequences [PDF]

International Journal of Systematic and Evolutionary Microbiology, 2004
Sequences of the gene encoding the beta-subunit of the RNA polymerase (rpoB) were used to delineate the phylogeny of the family Pasteurellaceae. A total of 72 strains, including the type strains of the major described species as well as selected field ...
Korczak, B., Frey, Joachim, Emler, Stefan, Korczak, Bozena, Frey, J., Christensen, H., Kuhnert, Peter, Kuhnert, P., Christensen, Henrik, Elmer, S., Frey J, Emler S, Kuhnert P, Korczak B, Christensen H   +14 more
core   +5 more sources

A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity [PDF]

PLoS ONE, 2012
Background: The 16S rRNA gene is the gold standard in molecular surveys of bacterial and archaeal diversity, but it has the disadvantages that it is often multiple-copy, has little resolution below the species level and cannot be readily interpreted in ...
Quince, Christopher, Christopher Quince, Quince Christopher, Vos, M., Pijl, Agata S., Vos Michiel, Michiel Vos, Pijl, A.S., Agata S. Pijl (183429), Quince, C., Pijl Agata S., Kowalchuk, G.A., Mattias de Hollander, Michiel Vos (63922), Mattias de Hollander (183437), Vos, Michiel, George A Kowalchuk, Kowalchuk George A., de Hollander, Mattias, George A. Kowalchuk (183442), Agata S Pijl, Kowalchuk, George A., Christopher Quince (178494), de Hollander, M., de Hollander Mattias   +24 more
core   +10 more sources

Detection of rpoB Gene Mutations in Mycobacterium Tuberculosis [PDF]

Journal of Applied Health Management and Technology, 2023
Perkembangan pengobatan infeksi tuberculosis memberikan harapan kesembuhan total akan tetapi seiring berjalanya waktu pengunaan obat berdampak pada munculnya strain Mycobacterium tuberculosis yang resisten terhadap obat peningkatan kecepatan diaknostik ...
Setyowatiningsih, Lilik, roniafriansya, roni, widodo, widodo, Riadi, Ahmad, Etevia Purlinda, Devi   +4 more
core   +2 more sources

Enhanced heterogeneity of rpoB in Mycobacterium tuberculosis found at low pH. [PDF]

Journal of Antimicrobial Chemotherapy, 2009
OBJECTIVES: The aim of this study was to gain an insight into the molecular mechanisms of the evolution of rifampicin resistance in response to controlled changes in the environment.
O'Sullivan, Denise M., Bose, Alpana, McHugh, Timothy D., O'Sullivan, Denise M, Allnutt, Jon, Hatch, Kim A, Jenkins, Claire, Bacon, Joanna, Gillespie, Stephen H., Arnold, Catherine, Gillespie, Stephen H, Hatch, Kim A., McHugh, Timothy D, Gillespie, Stephen H.; id_orcid   +13 more
core   +3 more sources

Genome‐Wide Analysis of Protein Binding to DNA in Plastids by ptChIP‐seq [PDF]

Current Protocols, Volume 6, Issue 11, November 2026.
Abstract Plastids contain their own genomes encoding essential components of the photosynthetic apparatus and gene expression machinery. Transcription in plastids is mediated by two RNA polymerases, nuclear‐encoded (NEP) and plastid‐encoded RNA polymerase (PEP).
V. Miguel Palomar, J. Jair López‐Morales, Sho Fujii, Andrzej T. Wierzbicki   +3 more
wiley   +2 more sources

rpoB Sequence Analysis of Cultured Tropheryma whippelii [PDF]

Journal of Clinical Microbiology, 2001
Until recently no isolate of Tropheryma whippelii was available, and therefore genetic studies were limited to those based on PCR amplification of conserved genes. In this study we determined the nucleotide sequence of
Michel Drancourt, Didier Raoult, Antoine Carlioz   +2 more
core   +3 more sources

The rpoB gene of Mycobacterium tuberculosis [PDF]

Antimicrobial Agents and Chemotherapy, 1994
A portion of the Mycobacterium tuberculosis gene encoding the beta subunit of RNA polymerase (rpoB) was amplified by PCR using degenerate oligonucleotides and used as a hybridization probe to isolate plasmid clones carrying the entire rpoB gene of M. tuberculosis H37Rv, a virulent, rifampin-susceptible strain.
L P, Miller, J T, Crawford, T M, Shinnick   +2 more
openaire   +2 more sources

Proofreading deficiency of Pol I increases the levels of spontaneous rpoB mutations in E. coli [PDF]

, 2011
The fidelity role of DNA polymerase I in chromosomal DNA replication in E. coli was investigated using the rpoB forward target. These experiments indicated that in a strain carrying a proofreading-exonuclease-defective form of Pol I (polAexo mutant) the ...
Jonczyk, Piotr, K. Makiela-Dzbenska, P. Jonczyk, Fijalkowska, Iwona J., Schaaper, Roel, R.M. Schaaper, I.J. Fijalkowska, Makiela-Dzbenska, Karolina   +7 more
core   +2 more sources