Results 101 to 110 of about 37,335 (238)

Integrated one‐pot CRISPR‐Cas detection: Addressing strategies and future prospects

Interdisciplinary Medicine, EarlyView.
Integrated one‐pot CRISPR‐Cas detection significantly simplifies workflows, reduces cross‐contamination risks, and shortens turnaround times, making it highly suitable for point‐of‐care testing (POCT) and resource‐limited settings. Key challenges include system compatibility issues between Cas proteins and isothermal amplification, limited multiplexing
Jian Zhou, Xuemei Ren, Yingjie Ren, Ruiping Wu, Ruiman Geng, Le Chen, Zhuo Li   +6 more
wiley   +1 more source

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

Effect of encapsulated purple sweet potato (Ipomoea batatas L.) peel extract on fresh goat cheese: quality and polyphenolic and color stability during refrigerated storage

Journal of the Science of Food and Agriculture, EarlyView.
Abstract BACKGROUND The aim of this work was to characterize the polyphenolic profile of encapsulated purple sweet potato peel extract (PSPPE), to assess the effects of its addition (1.5 and 3 g L−1) on the color, texture, microbiological quality, microstructure, and sensory attributes of fresh goat cheese, and to monitor the polyphenolic profile and ...
Raquel Lucas‐González, Nuria Muñoz‐Tébar, Gema Martínez‐Navarrete, José Ángel Pérez‐Álvarez, Eduardo Fernández, José Manuel Lorenzo, Juana Fernández‐López, Manuel Viuda‐Martos   +7 more
wiley   +1 more source

Micro-scale control of oligodendrocyte morphology and myelination by the intellectual disability-linked protein acyltransferase ZDHHC9

eLife
Mutations in the X-linked ZDHHC9 gene cause cognitive deficits in humans, with a subset of patients suffering from epilepsy. X-linked intellectual disability (XLID) is often ascribed to neuronal deficits, but here we report that expression of human and ...
Hey-Kyeong Jeong, Estibaliz Gonzalez-Fernandez, Ilan Crawley, Julia M Coakley, Jinha Hwang, Dale DO Martin, Shernaz X Bamji, Jong-Il Kim, Shin H Kang, Gareth M Thomas   +9 more
doaj   +1 more source

Curly protein nanofibril systems for improving thermal stability and copigmentation of hibiscus anthocyanins

Journal of the Science of Food and Agriculture, EarlyView.
Abstract BACKGROUND Anthocyanins are attractive natural colorants for clean‐label food applications; however, their widespread use is limited by poor thermal stability. Protein nanofibrils have recently emerged as promising natural stabilizers because of their high surface area and ability to interact with anthocyanins through non‐covalent interactions.
Vildan Eyiz, İsmail Tontul, Selman Türker   +2 more
wiley   +1 more source

KAT2A Promotes the Progression of Renal Cell Carcinoma by Regulating the Succinylation of SERPINE2

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Epithelial‐mesenchymal transition (EMT) and angiogenesis are critical drivers of renal cell carcinoma (RCC) progression, yet their upstream regulatory mechanisms remain incompletely understood. This study aimed to investigate the role of KAT2A in RCC and elucidate the underlying molecular mechanism by which it promotes tumor progression.
Chang‐Cheng Liu, Xue‐Dong Li
wiley   +1 more source

Analytical Approaches to Address Challenges in the Analysis of Cannabinoids in Vascular Matrices Using Mass Spectrometry

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Phytocannabinoids are bioactive metabolites derived from the Cannabis sativa plant. They have garnered attention due to their recreational uses and therapeutic potential. Although various analytical strategies have been employed for their analysis, mass spectrometry (MS) coupled to chromatographic separation is superior due to its sensitivity ...
Radwa Mahmoud, Robert B. Laprairie, Anas El‐Aneed   +2 more
wiley   +1 more source

Enzyme-centric chemoproteomics reveals isomer-specific S-acylation modification networks

Nature Communications
Protein lipidation is a critical post-translational modification, but the relationship between the fine structure of fatty acids (FAs) and the specificity of lipidation remains largely unexplored.
Pengfei Wu, Wenjing Nie, Yinsheng Wu, Xiao Huang, Qiongqiong Wan, Shanshan Li, Suming Chen   +6 more
doaj   +1 more source

Curing Behavior and Adhesion Performance of a One‐Component Epoxy Resin Based on a Dual‐Locked Thermal Latent Curing Agent

Journal of Polymer Science, EarlyView.
In this study, curing behavior of epoxy resins using a dual‐locked thermal latent curing agent (DCA) was analyzed using DSC and FT‐NIR. The DCA was successfully dissolved in the bisphenol A type epoxy resin (DGEBA). Furthermore, the prepared one‐component epoxy resin was used as an adhesive and achieved excellent adhesive strength (> 25 MPa).
Yukai Ito, Daisuke Aoki, Koji Arimitsu
wiley   +1 more source

The continuing significance of chiral agrochemicals

Pest Management Science, Volume 81, Issue 4, Page 1697-1716, April 2025.
In the time frame 2018–2023, around 43% of the 35 chiral agrochemicals introduced to the market (herbicides, fungicides, insecticides, acaricides, and nematicides) contain one or more stereogenic centers in the molecule, and almost 69% of them have been marketed as racemic mixtures of enantiomers or stereoisomers.
Peter Jeschke
wiley   +1 more source