Results 71 to 80 of about 1,124 (173)

Immunolocalization of the saposin-like insert of plant aspartic proteinases exhibiting saposin C activity. Expression in young flower tissues and in barley seeds

, 2005
The plant- specific insert ( PSI) of cypro11 gene- encoding cyprosin, an aspartic proteinase from Cynara cardunculus, has been cloned by polymerase chain reaction ( PCR) into a bacterial expression vector.
Marttila, S, Al-Karadaghi, Salam, Picaud, S, Hiraiwa, M, Brodelius, M, Brodelius, P E   +5 more
core   +1 more source

Stimulation of Acid Ceramidase Activity by Saposin D

Archives of Biochemistry and Biophysics, 1994
Ceramide is ubiquitously present in plasma membranes and plays a pivotal role in metabolism of sphingolipids. In addition, ceramide and its hydrolytic product, sphingosine, may have important roles as second messengers for cell function and survival. Ceramide is hydrolyzed by both acid and alkaline ceramidase. In this investigation, saposin D was found
N, Azuma, J S, O'Brien, H W, Moser, Y, Kishimoto   +3 more
openaire   +2 more sources

Structural similarities reveal an expansive conotoxin family with a two‐finger toxin fold

Protein Science, Volume 34, Issue 11, November 2025.
Abstract Venomous animals have evolved a diverse repertoire of toxins with considerable pharmaceutical potential. The rapid evolution of peptide toxins, such as the conotoxins produced by venomous marine cone snails, often complicates efforts to infer their evolutionary relationships based solely on sequence information.
Muhammad Saad Khilji, Celeste M. Hackney, Thomas L. Koch, Arik J. Hone, Aymeric Rogalski, Maren Watkins, Jortan Tun, J. Michael McIntosh, Baldomero Olivera, Helena Safavi‐Hemami, Kaare Teilum, Lars Ellgaard   +11 more
wiley   +1 more source

STRUCTURAL-ANALYSIS OF SAPOSIN-C AND SAPOSIN-B - COMPLETE LOCALIZATION OF DISULFIDE BRIDGES

, 1995
Saposins A, B, C, and D are a group of homologous glycoproteins derived from a single precursor, prosaposin, and apparently involved in the stimulation of the enzymatic degradation of sphingolipids in lysosomes. All saposins have six cysteine residues at
A. Amoresano, R. Ciaffoni, F. Zappacosta, R. Salvioli, R. Siciliano, P. Pucci, A. M. Vaccaro, A. Barca, M. Tatti, MARAS, Bruno   +9 more
core   +1 more source

Generation and Auditory Phenotypic Characterization of Prps1 p.Ala87Thr Mouse Knock‐In Model for Human DFNX1 Deafness

Clinical Genetics, Volume 108, Issue 5, Page 566-575, November 2025.
Our mouse model with a c.259G>A transition in PRPS1 showed a significant decrease in the number of hair cells and SGN counts at 48 weeks of age and a reduction in Prps1 enzymatic activity in the KI mouse. This model will serve as a valuable tool for developing therapeutic strategies.
Denise Yan, M'hamed Grati, Rahul Mittal, Yi‐Zhou Quan, Wan Du, Zheng‐Yi Chen, Xue Zhong Liu   +6 more
wiley   +1 more source

Target lysis by cholesterol extraction is a rate limiting step in the resolution of phagolysosomes

European Journal of Cell Biology
The ongoing phagocytic activity of macrophages necessitates an extraordinary capacity to digest and resolve incoming material. While the initial steps leading to the formation of a terminal phagolysosome are well studied, much less is known about the ...
Dante Barreda, Sergio Grinstein, Spencer A. Freeman   +2 more
doaj   +1 more source

Host Transcriptomics Reveal Reduction in Defence‐Reproduction Trade‐Offs During Coinfection

Molecular Ecology, Volume 34, Issue 21, November 2025.
ABSTRACT During infection, hosts may shift resources away from reproduction towards immune defence. It is unclear to what degree these costly trade‐offs can be alleviated during protective coinfections, whereby antagonism between parasites reduces disease severity. We used transcriptomics to assess the extent to which host gene expression reflected the
Ian Will, Emily J. Stevens, Kayla C. King, Kieran A. Bates   +3 more
wiley   +1 more source

Management of Gaucher Disease Type 1 in a Resource‐Limited Setting: A Pediatric Case Study

Clinical Case Reports, Volume 13, Issue 10, October 2025.
ABSTRACT This case report depicts the management of an 8‐year‐old male with Gaucher Disease Type 1, manifesting as massive splenomegaly, anemia, and skeletal involvement in a resource‐constrained environment. Treated with splenectomy due to the absence of enzyme replacement therapy, it underscores the necessity for enhanced therapeutic access and ...
Bipesh Kumar Shah, Diwakar Koirala, Bivek Mishra, Ramesh Sapkota, Manjil Basnet   +4 more
wiley   +1 more source

Palmitoyl‐Protein Thioesterase 1 (PPT1) Protein, Linked to Neuronal Ceroid Lipofuscinosis 1, Is a Major Constituent of Ageing‐Related Human Neuronal Lipofuscin

Neuropathology and Applied Neurobiology, Volume 51, Issue 5, October 2025.
ABSTRACT Proteomics of laser‐dissected lipofuscin from aged, healthy brains reveals Palmitoyl‐Protein Thioesterase 1 (PPT1) and other CLN proteins as constituents. PPT1 is increasingly sequestered to lipofuscin during ageing. Protein sequestering into lipofuscin may contribute to physiological neuronal ageing.
Max Anstötz, Sarah Tschirner, Caroline May, Steffen Kösters, Christine Martin, Svenja Caspers, Eleonora Aronica, Hans Jürgen Bidmon, Katrin Marcus, Carsten Korth   +9 more
wiley   +1 more source

Function of saposin C in the reconstitution of glucosylceramidase by phosphatidylserine liposomes

FEBS Letters, 1993
The function of saposin C (Sap C), a glucosylceramidase activator protein, in the enzyme stimulation by phosphatidylserine (PS) liposomes has been investigated. Using gel filtration experiments evidence was obtained for Sap C binding to PS large unilamellar vesicles (LUV) but not to glucosylceramidase.
Vaccaro, A. M., Tatti, M., Ciaffoni, F., Salvioli, R., MARAS, Bruno, Barca, A.   +5 more
openaire   +3 more sources