Results 111 to 120 of about 52,178 (281)

Engineered CAR‐NKT Extracellular Vesicles Suppress Tumor Progression and Enhance Antitumor Immunity

Advanced Science, EarlyView.
TM4SF1‐nanobody engineered CAR‐natural killer T–derived extracellular vesicles (CARTM4SF1‐EVs) provide a cell‐free alternative to CAR‐NKT therapy, achieving potent, targeted antitumor activity with reduced toxicity. CARTM4SF1‐EVs induce immunogenic cell death, remodel the tumor microenvironment, and enhance CD8⁺ T‐cell antitumor immunity.
Xiaopei Hao, Chengming Qu, Yanzhao Zhou, Xiaoqian Wang, Xiangjun Qian, Xun Chen, Feng Han, Xiaokai Zhang, Yiyi Ji, Han Li, ChengWei Ju, Peng Xia, Weiwei Tang, Hao Zhuang, Jinxue Zhou   +14 more
wiley   +1 more source

Radiosensitization and growth inhibition of cancer cells mediated by an scFv antibody gene against DNA-PKcs in vitro and in vivo

Radiation Oncology, 2010
Background Overexpression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is commonly occurred in cancers and causes radioresistance and poor prognosis. In present study, the single-chain variable antibody fragments (scFv) targeting DNA-PKcs
Du Li, Zhou Li-Jun, Pan Xiu-Jie, Wang Yu-Xiao, Xu Qin-Zhi, Yang Zhi-Hua, Wang Yu, Liu Xiao-Dan, Zhu Mao-Xiang, Zhou Ping-Kun   +9 more
doaj   +1 more source

Rudimentary G-Quadruplex-Based Telomere Capping In Saccharomyces Cerevisiae [PDF]

, 2011
Telomere capping conceals chromosome ends from exonucleases and checkpoints, but the full range of capping mechanisms is not well defined. Telomeres have the potential to form G-quadruplex (G4) DNA, although evidence for telomere G4 DNA function in vivo ...
A De Cian, A Piazza, A Risitano, A Risitano, A Sfeir, AC Vallur, AN Lane, B Garvik, B Saccà, BR Linger, C Frei, C Ribeyre, CI Nugent, D Bonetti, D Gomez, DA Bernstein, David C Schultz, David Monchaud, EA Venczel, EP Mimitou, F Brad Johnson, H Ding, H Fernando, IM Pedroso, J Kypr, J Lin, J Lu, J Ren, J-L Mergny, JA Cobb, Jasmine S Smith, JD Frantz, John M Nicoludis, JR Mullen, JS Smith, K Förstemann, K Paeschke, L Maringele, L Oganesian, Lara Abramowitz, LD Nelson, Liliya A Yatsunyk, LL Sandell, M Downey, Marie-Paule Teulade-Fichou, Mark S Garcia, MD Huber, MD Huber, MD Vodenicharov, MK Zubko, MK Zubko, ML Kozak, ML Zhang, MW Van Dyke, N Grandin, N Hayashi, P Phatak, PM Watt, Qijun Chen, R Giraldo, Ramon Kranaster, RJ Michelson, S Burge, SG Addinall, SG Hershman, Shankar Balasubramanian, TR Salas, W Palm, Y Li, Y-C Tsai, Z Liu, Z Zhu   +71 more
core   +2 more sources

Engineering Immune Cell to Counteract Aging and Aging‐Associated Diseases

Advanced Science, EarlyView.
This review highlights a paradigm shift in which advanced immune cell therapies, initially developed for cancer, are now being harnessed to combat aging. By engineering immune cells to selectively clear senescent cells and remodel pro‐inflammatory tissue microenvironments, these strategies offer a novel and powerful approach to delay age‐related ...
Jianhua Guo, Lanjie Lei, Ying Jin, Lan Su, Shumao Cui, Liyun Shi   +5 more
wiley   +1 more source

Hevea genetic transformation using a single-chain variable fragment (scFv) specific to cassiicolin, the causal agent of Corynespora leaf disease [PDF]

, 2011
The pathogenic fungus Corynespora cassiicola a serious threat to rubber trees (Hevea brasiliensis); the infected leaves develop necrotic lesions and abscise, leaving the tree unproductive. The destructiveness of C.
Ezam Badarudin, Badrul, Kadir, Rusni A., Malik, Adeel, Nathan, Sheila, Pujade-Renaud, Valérie, Sunderasan, E., Yeang, Hoong Yeet   +6 more
core  

Evaluation of METase-pemetrexed-loaded PEG–PLGA nanoparticles modified with anti-CD133–scFV for treatment of gastric carcinoma

Bioscience Reports, 2017
PEG–PLGA nanoparticles (NPs) modified with anti-CD133 and tumor-targeting single-chain antibody fragment (scFV–NPs) for systemic delivery of methioninase (METase) and pemetrexed for gastric carcinoma were successfully formulated.
L. Xin, Hou-Ting Zhang, Wei-feng Yang, Yi-Fan Li, Chuan Liu   +4 more
semanticscholar   +1 more source

Specific Detection of His-Tagged Proteins with Recombinant Anti-His Tag scFv-Phosphatase or scFv-Phage Fusions

BioTechniques, 1997
Using a cell-bound immunogen, we have generated a monoclonal antibody, 3D5, that recognizes carboxy-terminal oligo-histidine tags (His tags) on a wide variety of proteins. From this monoclonal antibody, we have generated a single-chain fragment of the variable domains (scFv), a dimeric scFv-alkaline phosphatase fusion and an oligovalent scFv-display ...
P. Lindner, K. Bauer, A. Krebber, L. Nieba, E. Kremmer, C. Krebber, A. Honegger, B. Klinger, R. Mocikat, A. Plückthun   +9 more
openaire   +3 more sources

A Murine Bispecific Antibody Efficiently Redirects T Cells Against Calr Mutated Stem Cells In Vivo

American Journal of Hematology, EarlyView.
ABSTRACT Calreticulin (CALR) mutations are prevalent in 20%–30% of patients with BCR::ABL1‐negative myeloproliferative neoplasms (MPN). Mutant calreticulin (mutCALR), presented by the thrombopoietin receptor (MPL, also known as TPOR or CD110) on the surface of the disease‐initiating MPN progenitors, represents an ideal target for curative ...
Shengen Xiong, Tamara Wais, Cecilia Varga, Christina Schueller, Sarada Achyutuni, Robert Kralovics   +5 more
wiley   +1 more source

Targeting fibroblast activation protein in tumor stroma with chimeric antigen receptor T cells can inhibit tumor growth and augment host immunity without severe toxicity. [PDF]

, 2014
The majority of chimeric antigen receptor (CAR) T-cell research has focused on attacking cancer cells. Here, we show that targeting the tumor-promoting, nontransformed stromal cells using CAR T cells may offer several advantages.
Albelda, Steven M., Antzis, Michael, Cotner, Cody E., Durham, Amy C., Johnson, Laura A., June, Carl H., Kapoor, Veena, Lo, Albert, Majumdar, Rajrupa S., Puré, Ellen, Scholler, John, Solomides, MD, Charalambos C., Sun, Jing, Wang, Liang-Chuan S.   +13 more
core   +2 more sources

An automated microfluidic biosensor with bispecific rabbit monoclonal antibody and nanozyme‐bioenzyme composite for dual antibiotic detection

BMEMat, EarlyView.
This study introduces an automated microfluidic biosensor for simultaneous detection of florfenicol and thiamphenicol in environmental water. By integrating a phage‐screened bispecific rabbit monoclonal antibody with a nanozyme‐bioenzyme composite, the system achieves sensitive dual‐antibiotic detection. Coupled with smartphone‐based sensing, it offers
Fuyuan Zhang, Yang Liu, Juntao Wang, Haichao Zhang, Wenlong Yu, Yaxin Sang, Yun Liu, Guangze Yang, Fang Gao, Shuo Wang, Shilun Feng, Xianghong Wang   +11 more
wiley   +1 more source