Results 241 to 250 of about 52,178 (281)

High avidity scFv multimers; diabodies and triabodies

Journal of Immunological Methods, 1999
Multivalent recombinant antibody fragments provide high binding avidity and unique specificity to a wide range of target antigens and haptens. This review describes how careful choice of linker length between V-domains creates new types of Fv modules with size, flexibility and valency suited to in vivo imaging and therapy. Further, we review the design
P J, Hudson, A A, Kortt
openaire   +2 more sources

ScFv-CH3 and scFv-Fc Fusion Proteins

2001
Earlier chapters (Chapters 2–16) have presented methods for producing small engineered antibodies (notably, scFv) from hybridomas or through selection from combinatorial libraries. It is now possible to generate fragments with a vast spectrum of specificities, including against targets previously inaccessible through hybridoma technology.
openaire   +1 more source

Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv Yeast-Display Library

2015
B7-H4 (VTCN1, B7x, B7s) is an inhibitory modulator of T-cell response implicated in antigen tolerization. As such, B7-H4 is an immune checkpoint of potential therapeutic interest. To generate anti-B7-H4 targeting reagents, we isolated antibodies by differential cell screening of a yeast-display library of recombinant antibodies (scFvs) derived from ...
Denarda, Dangaj, Nathalie, Scholler
openaire   +2 more sources

Isolation of single chain variable fragment (scFv) specific for Cry1C toxin from human single fold scFv libraries

Toxicon, 2012
As bioinsecticides Bacillus thuringiensis Cry1C δ-endotoxins also have been used in genetically modified crops worldwide since last century. In this study, single chain variable fragments (scFvs), which could specifically recognize and detect Cry1C in food samples, were isolated from naive phage displayed human antibody libraries (Tomlinson I + J) by ...
Yun, Wang, Xiao, Zhang, Cunzheng, Zhang, Yuan, Liu, Xianjin, Liu   +4 more
openaire   +2 more sources

Screening and Molecular Evolution of a Single Chain Variable Fragment Antibody (scFv) against Citreoviridin Toxin.

Journal of Agricultural and Food Chemistry, 2016
Citreoviridin (CIT), a small food-borne mycotoxin produced by Penicillium citreonigrum, is generally distributed in various cereal grains and farm crop products around the world and has caused cytotoxicity as an uncompetitive inhibitor of ATP hydrolysis.
Rongzhi Wang, Xiaosong Gu, Zhen-Hong Zhuang, Yanfang Zhong, Hang Yang, Shihua Wang   +5 more
semanticscholar   +1 more source

Efficient display of scFv antibodies on bacteriophage lambda

Journal of Immunological Methods, 2006
In the present work we demonstrate the efficient display of functional scFv antibodies on the bacteriophage lambda capsid. A single-chain (scFv) anti-CEA antibody gene was cloned in two different vectors to obtain fusion of the scFv antibody to the N- or C-terminus of the bacteriophage lambda capsid protein D (gpD). Lambda bacteriophage assembly occurs
VACCARO P, PAVONI E, MONTERIU' G, PUCCI A, FELICI, Franco, MINENKOVA O.   +5 more
openaire   +3 more sources

Cytotoxic Tumor Targeting With scFv Antibody-Modified Liposomes

2004
Specific targeting of liposome-formulated cytotoxic drugs or antigens to receptors expressed selectively on target cells represents an effective strategy for increasing the pharmacological efficacy of the delivered molecules. We have developed a feasible technique to selectively attach antibodies and fragments thereof, but also small-mol-wt ligands ...
Marty, Cornelia, Schwendener, Reto A
openaire   +3 more sources

High-Level Periplasmic Expression and Purificationof scFvs

2003
The isolation of recombinant antibodies by phage display naturally leads to experiments to evaluate their biological and immunological properties. Although crude preparations may have their value in initial studies, the need often exists for highly purified protein that can be tested in vivo.
openaire   +3 more sources

Functional Minipreps of scFv-Antibody Fragments

2001
Complete immunoglobulin molecules cannot be produced in Escherichia coli, but different strategies have been developed to express antibody fragments like single-chain antibodies (scFvs), disulfide stabilized antibodies (dsFvs), Fab fragments, bispecific antibodies (bsAbs) or various immunotoxins (overview in Breitling and Dubel, 1999).
Andreas Schmiedl, Stefan Dübel
openaire   +1 more source

Anti-HER2 scFv Expression in Escherichia coli SHuffle®T7 Express Cells: Effects on Solubility and Biological Activity

Molecular Biotechnology, 2020
M. Ahmadzadeh, Farzaneh Farshdari, L. Nematollahi, M. Behdani, E. Mohit   +4 more
semanticscholar   +1 more source