Results 251 to 260 of about 52,178 (281)
Some of the next articles are maybe not open access.
2010
Aim. To develop approach for detection of scFv and their complexes with antigens. Methods. The fusion proteins, which include sequences of scFv and staphylococcal protein A, were constructed and the obtained bifunctional molecules were immunochemically analysed. Results. It was shown, that scFv fused with protein A and their complexes with antigens are
openaire +1 more source
Aim. To develop approach for detection of scFv and their complexes with antigens. Methods. The fusion proteins, which include sequences of scFv and staphylococcal protein A, were constructed and the obtained bifunctional molecules were immunochemically analysed. Results. It was shown, that scFv fused with protein A and their complexes with antigens are
openaire +1 more source
[Construction of human phage-displayed scFv library and selection of the ScFv against rabies virus].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2006To construct a human phage displayed single chain Fv antibody (scFv) library and screen specific scFv against rabies virus.Using a phage-display technique, IgVH and IgVL genes were cloned from peripheral blood lymphocytes from three donors immunized with the WISTAR PM strain vaccine.
Xiao-ling, Zhao +4 more
openaire +1 more source
Development of a SARS-CoV-2-specific biosensor for antigen detection using scFv-Fc fusion proteins
Biosensors and Bioelectronics, 2021Keun Bon Ku +2 more
exaly
2019
An inexpensive and effective refolding method for ScFv-CBD fusion protein recovery from E. coli inclusion bodies has been developed. The principle of the proposed method is the stepwise dilution of solubilized ScFv-CBD in the presence of 6 M guanidine hydrochloride with refolding buffer containing L-arginine and glutathione.
openaire +1 more source
An inexpensive and effective refolding method for ScFv-CBD fusion protein recovery from E. coli inclusion bodies has been developed. The principle of the proposed method is the stepwise dilution of solubilized ScFv-CBD in the presence of 6 M guanidine hydrochloride with refolding buffer containing L-arginine and glutathione.
openaire +1 more source
Expression of scFvs and scFv Fusion Proteins in Eukaryotic Cells
2003Michelle, de Graaf +3 more
openaire +2 more sources
Engineering scFvs for Improved Stability
2003Partha S, Chowdhury, George, Vasmatzis
openaire +2 more sources
Anti-PEG scFv corona ameliorates accelerated blood clearance phenomenon of PEGylated nanomedicines
Journal of Controlled Release, 2021Cheng Li, Xiaoli Wei, Weiyue Lu
exaly