Results 71 to 80 of about 35,905 (251)

Screening of a ScFv Antibody With High Affinity for Application in Human IFN-γ Immunoassay

Frontiers in Microbiology, 2018
Interferon gamma (IFN-γ), a signal proinflammatory cytokine secreted by immune cell, and plays a critical role in the pathogenesis and progression of many diseases.
Hang Yang, Yanfang Zhong, Juncheng Wang, Qinghong Zhang, Xiulan Li, Sumei Ling, Shihua Wang, Rongzhi Wang   +7 more
semanticscholar   +1 more source

Rewriting Tumor Immunity With Bacterial Membrane Vesicles: A Versatile and Potent Therapeutic Strategy

Advanced NanoBiomed Research, EarlyView.
Bacterial membrane vesicles (BMVs) emerge as promising platforms for cancer immunotherapy owing to their intrinsic adjuvant properties and tunable cargo delivery capabilities. Their ability to modulate the tumor microenvironment, enhance antitumor immune responses, and support personalized therapeutic strategies highlights their growing potential as ...
Md Sifat Rahi, Md Mijanur Rahman, Asma Talukder, Urooj Yousaf Virk, Jennifer C. Wilson, Nigel McMillan, Ming Q. Wei   +6 more
wiley   +1 more source

Anti-CTGF single-chain variable fragment dimers inhibit human airway smooth muscle (ASM) cell proliferation by down-regulating p-Akt and p-mTOR levels.

PLoS ONE, 2014
Connective tissue growth factor (CTGF) contributes to airway smooth muscle (ASM) cell hyperplasia in asthma. Humanized single-chain variable fragment antibody (scFv) was well characterized as a CTGF antagonist in the differentiation of fibroblast into ...
Wei Gao, Liting Cai, Xudong Xu, Juxiang Fan, Xiulei Xue, Xuejiao Yan, Qinrong Qu, Xihua Wang, Chen Zhang, Guoqiu Wu   +9 more
doaj   +1 more source

Recombinant production of the antibody fragment D1.3 scFv with different Bacillus strains

Microbial Cell Factories, 2017
BackgroundDifferent strains of the genus Bacillus are versatile candidates for the industrial production and secretion of heterologous proteins. They can be cultivated quite easily, show high growth rates and are usually non-pathogenic and free of endo ...
Antonia Lakowitz, R. Krull, R. Biedendieck   +2 more
semanticscholar   +1 more source

Pacific Biosciences Sequencing and IMGT/HighV-QUEST Analysis of Full-Length Single Chain Fragment Variable from an In Vivo Selected Phage-Display Combinatorial Library

Frontiers in Immunology, 2017
Phage-display selection of immunoglobulin (IG) or antibody single chain Fragment variable (scFv) from combinatorial libraries is widely used for identifying new antibodies for novel targets.
Audrey Hemadou, Véronique Giudicelli, Melissa Laird Smith, Marie-Paule Lefranc, Patrice Duroux, Sofia Kossida, Cheryl Heiner, N. Lance Hepler, John Kuijpers, Alexis Groppi, Jonas Korlach, Philippe Mondon, Florence Ottones, Marie-Josée Jacobin-Valat, Jeanny Laroche-Traineau, Gisèle Clofent-Sanchez   +15 more
doaj   +1 more source

Development of an Indirect Competitive ELISA for Glycocholic Acid Based on Chicken Single-Chain Variable Fragment (scFv) Antibodies

Analytical Chemistry, 2017
Glycocholic acid (GCA) is an important metabolite of bile acids, whose urine levels are expected to be a specific diagnostic biomarker for hepatocellular carcinoma (HCC).
Xiping Cui, N. Vasylieva, Panpan Wu, B. Barnych, Jun Yang, Ding Shen, Qiyi He, S. Gee, Suqing Zhao, B. Hammock   +9 more
semanticscholar   +1 more source

A modular IgG-scFv bispecific antibody topology [PDF]

Protein Engineering, Design and Selection, 2009
Here we present a bispecific antibody (bsAb) format in which a disulfide-stabilized scFv is fused to the C-terminus of the light chain of an IgG to create an IgG-scFv bifunctional antibody. When expressed in mammalian cells and purified by one-step protein A chromatography, the bsAb retains parental affinities of each binding domain, exhibits IgG-like ...
Kelly Davis, Orcutt, Margaret E, Ackerman, Maryelise, Cieslewicz, Emmanuel, Quiroz, Adrian L, Slusarczyk, John V, Frangioni, K Dane, Wittrup   +6 more
openaire   +2 more sources

Simple, mild, one-step labelling of proteins with gallium-68 using a tris(hydroxypyridinone) bifunctional chelator: a 68Ga-THP-scFv targeting the prostate-specific membrane antigen

EJNMMI Research, 2017
BackgroundLabelling proteins with gallium-68 using bifunctional chelators is often problematic because of unsuitably harsh labelling conditions such as low pH or high temperature and may entail post-labelling purification.
Saima Nawaz, G. Mullen, K. Sunassee, J. Bordoloi, P. Blower, J. Ballinger   +5 more
semanticscholar   +1 more source

Using Synthetic Glycans to Investigate Anti‐Glycan Antibodies and Explore Their Medical Potential

Angewandte Chemie International Edition, EarlyView.
Anti‐glycan antibodies are essential in health and disease. Access to novel glycan structures paves the way for progress in antibody profiling for biomarker discovery, antibody development, and vaccine design. We summarize the strategies to synthesize and utilize synthetic glycans for the development and application of anti‐glycan antibodies in basic ...
Fabienne Weber, Aina Valenti, Jiří Ledvinka, Oren Moscovitz, Peter H. Seeberger   +4 more
wiley   +1 more source

Radiosensitization and growth inhibition of cancer cells mediated by an scFv antibody gene against DNA-PKcs in vitro and in vivo

Radiation Oncology, 2010
Background Overexpression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is commonly occurred in cancers and causes radioresistance and poor prognosis. In present study, the single-chain variable antibody fragments (scFv) targeting DNA-PKcs
Du Li, Zhou Li-Jun, Pan Xiu-Jie, Wang Yu-Xiao, Xu Qin-Zhi, Yang Zhi-Hua, Wang Yu, Liu Xiao-Dan, Zhu Mao-Xiang, Zhou Ping-Kun   +9 more
doaj   +1 more source