Results 71 to 80 of about 35,719 (245)

Characterization and analysis of scFv-IgG bispecific antibody size variants

mAbs, 2018
Bispecific antibodies are an emergent class of biologics that is of increasing interest for therapeutic applications. In one bispecific antibody format, single-chain variable fragments (scFv) are linked to or inserted in different locations of an intact ...
Mingyan Cao, Chunlei Wang, W. Chung, Dana Motabar, Jihong Wang, Elizabeth Christian, Shihua Lin, A. Hunter, Xiangyang Wang, Dengfeng Liu   +9 more
semanticscholar   +1 more source

CCL3 and IL‐7 Synergistically Enhance CAR‐T Efficacy in Solid Tumors

Advanced Science, EarlyView.
Synergistic delivery of CCL3 and IL‐7 by CAR‐T cells overcomes the immunosuppressive tumor microenvironment by enhancing cell infiltration, survival, and local immune reprogramming. This also establishes positive feedback that amplifies anti‐tumor activity and memory responses in both CAR‐T and endogenous T cells, presenting a robust therapeutic ...
Huanpeng Chen, Xiaoyun Luo, Huixin Gao, Yujing Ke, Yu Zhan, Xiaoting Xu, Xiaoxiao Xiong, Fengjiao Wei, Si Yang, Zhonghua Liu, Bolan Yu, Zhaofeng Huang, Yingjie Bian   +12 more
wiley   +1 more source

Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes

Journal of Nanobiotechnology, 2018
BackgroundRecent advances in nanomedicine have shown the great interest of active targeting associated to nanoparticles. Single chain variable fragments (scFv) of disease-specific antibodies are very promising targeting entities because they are small ...
Christophe Alric, K. Hervé-Aubert, N. Aubrey, Souad Melouk, L. Lajoie, W. Même, S. Même, Y. Courbebaisse, A. Ignatova, A. Feofanov, I. Chourpa, E. Allard-Vannier   +11 more
semanticscholar   +1 more source

Hemoglobin's α‐Helix‐to‐β‐Sheet Transition Enables Targeted mRNA Delivery to the Lung

Advanced Science, EarlyView.
Acidic heating converts hemoglobin into β‐sheet‐rich fibrils with positively charged surfaces. These hemoglobin fibrils electrostatically complex IL‐11 scFv mRNA, selectively bind circulating platelets, and hitchhike to the lung, where they are enriched in fibroblasts.
Xihua Liu, Saiya Li, Guodong Wu, Shuangjian Li, Long Shuang Huang, Xiaoyang Li, Yiguo Zhao, Wei Lu, Cuixia Sun, Qin Cao, Yapeng Fang, Yiping Cao   +11 more
wiley   +1 more source

PET Imaging of 64Cu-DOTA-scFv-Anti-PSMA Lipid Nanoparticles (LNPs): Enhanced Tumor Targeting over Anti-PSMA scFv or Untargeted LNPs

Nuclear Medicine and Biology, 2017
Introduction Single chain (scFv) antibodies are ideal targeting ligands due to their modular structure, high antigen specificity and affinity. These monovalent ligands display rapid tumor targeting but have limitations due to their fast urinary clearance.
P. Wong, Lin Li, Junie Chea, M. K. Delgado, Desiree M Crow, E. Poku, B. Szpikowska, Nicole Bowles, D. Channappa, D. Colcher, J. Wong, J. Shively, P. Yazaki   +12 more
semanticscholar   +1 more source

TDP‐43 Aggregation: The Healthy‐Toxic Balance of the Prion‐Like Domain

Advanced Science, EarlyView.
TDP‐43 function relies on a delicate balance between reversible phase‐separated states and irreversible aggregation. Under physiological conditions, TDP‐43 forms dynamic droplets and oligomers that support normal cellular functions. In pathological contexts, this balance shifts toward aberrant aggregation, leading to toxic species.
Luca Zangrando, Emanuele Buratti, Francesca Paron   +2 more
wiley   +1 more source

Directed evolution of human scFvs in DT40 cells [PDF]

Protein Engineering Design and Selection, 2015
Cells that constitutively diversify their immunoglobulin genes can be used for selection of novel antibodies and for refining existing affinities and specificities. Here, we report an adaptation of the chicken DT40 system wherein its capacity for somatic hypermutation is harnessed to evolve human antibodies expressed as single-chain variable fragments (
Lim, Alfred W.Y., Williams, Gareth T., Rada, Cristina, Sale, Julian E.   +3 more
openaire   +2 more sources

A conjugate of an anti-midkine single-chain variable fragment to doxorubicin inhibits tumor growth

Brazilian Journal of Medical and Biological Research, 2012
Doxorubicin (DOX) was conjugated to a single-chain variable fragment (scFv) against human midkine (MK), and the conjugate (scFv-DOX) was used to target the chemotherapeutic agent to a mouse solid tumor model in which the tumor cells expressed high levels
Shuli Zhao, Guangfeng Zhao, Hao Xie, Yahong Huang, Yayi Hou   +4 more
doaj   +2 more sources

Porcine kidney xenotransplantation: From primate models to clinical reality

Animal Models and Experimental Medicine, EarlyView.
In the face of a critical shortage of human donor kidneys for end‐stage renal disease patients, porcine kidney xenotransplantation has emerged as a viable solution. This field has navigated major hurdles, including immune rejection, physiological incompatibilities, potential biomechanical differences and the risk of cross‐species infection. To overcome
Zihang Guo, Ling Zhang, Shoulong Deng, Chuan Qin   +3 more
wiley   +1 more source

Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex

Advanced NanoBiomed Research, EarlyView.
We developed an assay to distinguish cellular binding from internalization. Compatible with microscopy and high‐throughput screening, the method identifies ligand‐mediated uptake. Applying top candidates to a protein‐based DNA carrier enhanced transfection efficiency, providing a rational strategy to improve non‐viral gene delivery systems.
David Scherer, Steffen Honrath, Jonas Kurmann, Alexander Klipp, Finn Brigger, Jean‐Christophe Leroux   +5 more
wiley   +1 more source