Results 51 to 60 of about 35,719 (245)

Purification of a Novel Anti-VEGFR2 Single Chain Antibody Fragmentand Evaluation of Binding Affinity by Surface Plasmon Resonance [PDF]

Advanced Pharmaceutical Bulletin, 2019
Purpose: The single-chain variable fragment (scFv) domain of antibodies is now considered asone of the therapeutic tools that can be produced by phage display technology (PDT).
Shirafkan Kordi, Mohammad Rahmati-Yamchi, Mehdi Asghari Vostakolaei, Abolfazl Barzegari, Jalal Abdolalizadeh   +4 more
doaj   +1 more source

A strategy for adenovirus vector targeting with a secreted single chain antibody. [PDF]

PLoS ONE, 2009
BACKGROUND:Successful gene therapy will require targeted delivery vectors capable of self-directed localization. In this regard, the use of antibodies or single chain antibody fragments (scFv) in conjunction with adenovirus (Ad) vectors remains an ...
Joel N Glasgow, Galina Mikheeva, Victor Krasnykh, David T Curiel   +3 more
doaj   +1 more source

Development of an Anti-HER2 Single-Chain Variable Antibody Fragment Construct for High-Yield Soluble Expression in Escherichia coli and One-Step Chromatographic Purification

Biomolecules, 2023
Although single-chain variable fragment (scFv) is recognized as a highly versatile scaffold of recombinant antibody fragment molecules, its overexpression in Escherichia coli often leads to the formation of inclusion bodies.
Kyu Tae Byun, Boram Kim, Junmin Cho, Inbeom Lee, Myung Gu Lee, Dongsun Park, Tae-Bong Kang, Hyung-Sik Won, Chan Gil Kim   +8 more
doaj   +1 more source

CAR T‐Cell Therapy in Neurology: A Scoping Review of Neuro‐Oncology, Autoimmune Diseases & Neurotoxicity

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Chimeric antigen receptor (CAR) T‐cell therapy has been investigated in neurological diseases, encompassing both central nervous system malignancies and autoimmune disorders, thereby extending its application beyond hematological cancers.
Omar Alqaisi, Mohammed Dibas, Patricia Tai, Ala Dibas, Osama Souied, Suhair Al‐Ghabeesh   +5 more
wiley   +1 more source

Affinity‐Tuned Albumin Hitchhiking Extends the Bioorthogonal Capture Window in Pretargeting Radiotheranostics

Advanced Healthcare Materials, EarlyView.
Pharmacokinetic engineering of clickable radioligands was achieved via albumin hitchhiking approach within a pretargeted radiotheranostics delivery platform. This approach reduced rapid renal elimination, expanded bioorthogonal capture window, boosted tumor uptake by ∼3.5‐fold, and improved the tumor‐to‐liver ratio by ∼6‐fold.
Xie He, Nicholas L. Fletcher, Gayathri R. Ediriweera, Yifei Zhu, James Humphries, Christopher B. Howard, Jiazheng Wang, Craig A. Bell, Kristian Kempe, Kristofer J. Thurecht   +9 more
wiley   +1 more source

A modular IgG-scFv bispecific antibody topology [PDF]

Protein Engineering, Design and Selection, 2009
Here we present a bispecific antibody (bsAb) format in which a disulfide-stabilized scFv is fused to the C-terminus of the light chain of an IgG to create an IgG-scFv bifunctional antibody. When expressed in mammalian cells and purified by one-step protein A chromatography, the bsAb retains parental affinities of each binding domain, exhibits IgG-like ...
Kelly Davis, Orcutt, Margaret E, Ackerman, Maryelise, Cieslewicz, Emmanuel, Quiroz, Adrian L, Slusarczyk, John V, Frangioni, K Dane, Wittrup   +6 more
openaire   +2 more sources

Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C [PDF]

The Ukrainian Biochemical Journal, 2015
The aim of this work was to obtain the recombinant single chain variable fragments of antibodies (scFv) against human protein C, the key component of blood anticoagulation system. For this purpose a peptide that mimics a Pro144-Leu155 sequence of protein
O. S. Oliinyk, K. O. Palyvoda, N. E. Lugovskaya, D. V. Kolibo, E. V. Lugovskoy, S. V. Komisarenko   +5 more
doaj   +1 more source

Using near-infrared enhanced thermozyme and scFv dual-conjugated Au nanorods for detection and targeted photothermal treatment of Alzheimer's disease

Theranostics, 2019
Investigation of targeting inhibitors of Aβ aggregation, heme-Aβ peroxidase-like activity and efficient detectors of Aβ aggregation, are of therapeutic value and diagnostics significance for the treatment of Alzheimer's disease (AD).
Dongni Liu, Wei Li, Xiaoyu Jiang, S. Bai, Jiyang Liu, Xutong Liu, Yuhua Shi, Ziyu Kuai, W. Kong, R. Gao, Yaming Shan   +10 more
semanticscholar   +1 more source

Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity

Advanced Science, EarlyView.
This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley   +1 more source

Targeting GD2-Positive Tumor Cells by Pegylated scFv Fragment–Drug Conjugates Carrying Maytansinoids DM1 and DM4

Current Issues in Molecular Biology, 2023
Oligomerization of antibody fragments via modification with polyethylene glycol (pegylation) may alter their function and properties, leading to a multivalent interaction of the resulting constructs with the target antigen.
Daniel V. Kalinovsky, Irina V. Kholodenko, Elena V. Svirshchevskaya, Alexey V. Kibardin, Dmitry Yu. Ryazantsev, Fedor N. Rozov, Sergey S. Larin, Sergey M. Deyev, Roman V. Kholodenko   +8 more
doaj   +1 more source