Results 51 to 60 of about 44,698 (301)

Hemagglutinin sequence conservation guided stem immunogen design from influenza A H3 subtype [PDF]

, 2015
Seasonal epidemics caused by influenza A (H1 and H3 subtypes) and B viruses are a major global health threat. The traditional, trivalent influenza vaccines have limited efficacy because of rapid antigenic evolution of the circulating viruses.
Citron, Michael, Ferrara, Francesca, Flynn, Jessica A, Liang, Xiaoping, Mallajosyula, Vamsee V A, Temperton, Nigel J., Varadarajan, Raghavan   +6 more
core   +4 more sources

A strategy for adenovirus vector targeting with a secreted single chain antibody. [PDF]

PLoS ONE, 2009
BACKGROUND:Successful gene therapy will require targeted delivery vectors capable of self-directed localization. In this regard, the use of antibodies or single chain antibody fragments (scFv) in conjunction with adenovirus (Ad) vectors remains an ...
Joel N Glasgow, Galina Mikheeva, Victor Krasnykh, David T Curiel   +3 more
doaj   +1 more source

Development of an Anti-HER2 Single-Chain Variable Antibody Fragment Construct for High-Yield Soluble Expression in Escherichia coli and One-Step Chromatographic Purification

Biomolecules, 2023
Although single-chain variable fragment (scFv) is recognized as a highly versatile scaffold of recombinant antibody fragment molecules, its overexpression in Escherichia coli often leads to the formation of inclusion bodies.
Kyu Tae Byun, Boram Kim, Junmin Cho, Inbeom Lee, Myung Gu Lee, Dongsun Park, Tae-Bong Kang, Hyung-Sik Won, Chan Gil Kim   +8 more
doaj   +1 more source

Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system [PDF]

, 2002
The production and characterization of recombinant antibodies to aflatoxin B[SUB1] (AFB[SUB1]), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor ...
ANTHONY KILLARD, BERNADETTE MANNING, JANKNECHT R., KABAT E. A., LYNSEY DUNNE, NAWAZ S., NELSON R. D., PAUL DILLON, RICHARD O'KENNEDY, SCOTT P. M., STEPHEN DALY, VAN DER GAAG B., WAGNER V. T., YUAN Q.   +13 more
core   +1 more source

CAR T‐Cell Therapy in Neurology: A Scoping Review of Neuro‐Oncology, Autoimmune Diseases & Neurotoxicity

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Chimeric antigen receptor (CAR) T‐cell therapy has been investigated in neurological diseases, encompassing both central nervous system malignancies and autoimmune disorders, thereby extending its application beyond hematological cancers.
Omar Alqaisi, Mohammed Dibas, Patricia Tai, Ala Dibas, Osama Souied, Suhair Al‐Ghabeesh   +5 more
wiley   +1 more source

A modular IgG-scFv bispecific antibody topology [PDF]

Protein Engineering, Design and Selection, 2009
Here we present a bispecific antibody (bsAb) format in which a disulfide-stabilized scFv is fused to the C-terminus of the light chain of an IgG to create an IgG-scFv bifunctional antibody. When expressed in mammalian cells and purified by one-step protein A chromatography, the bsAb retains parental affinities of each binding domain, exhibits IgG-like ...
Kelly Davis, Orcutt, Margaret E, Ackerman, Maryelise, Cieslewicz, Emmanuel, Quiroz, Adrian L, Slusarczyk, John V, Frangioni, K Dane, Wittrup   +6 more
openaire   +2 more sources

Plant‐Produced Viral Nanoparticles Decorated with Nanobodies Against HER2 Improve Retention and Recruitment of Immune Cells in Solid Tumors

Advanced Healthcare Materials, EarlyView.
Potato virus X is a filamentous RNA plant virus that can be engineered into a molecular tool for cancer therapy. We produced genetically‐encoded virus‐derived nanoparticles decorated with nanobodies targeting cancer cell receptors, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2).
Enrique Lozano‐Sanchez, Miguel A. Moreno‐Gonzalez, Fernando Merwaiss, Nicole F. Steinmetz, José‐Antonio Daròs   +4 more
wiley   +1 more source

A novel synthetic trivalent single chain variable fragment (tri-scFv) construction platform based on the SpyTag/SpyCatcher protein ligase system

BMC Biotechnology, 2018
Background Advances in antibody engineering provide strategies to construct recombinant antibody-like molecules with modified pharmacokinetic properties.
Md. Kausar Alam, Michelle Brabant, Raja Solomon Viswas, Kris Barreto, Humphrey Fonge, C. Ronald Geyer   +5 more
doaj   +1 more source

Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C [PDF]

The Ukrainian Biochemical Journal, 2015
The aim of this work was to obtain the recombinant single chain variable fragments of antibodies (scFv) against human protein C, the key component of blood anticoagulation system. For this purpose a peptide that mimics a Pro144-Leu155 sequence of protein
O. S. Oliinyk, K. O. Palyvoda, N. E. Lugovskaya, D. V. Kolibo, E. V. Lugovskoy, S. V. Komisarenko   +5 more
doaj   +1 more source

Microfluidic Hydroporation Platform for Effective Allogeneic CAR‐T Cell Production and Subsequent Functional Cytotoxicity Analysis

Advanced Materials Technologies, EarlyView.
The hydroporator platform employs controlled hydrodynamic deformation for efficient mRNA and CRISPR/Cas9 delivery into primary human T cells, enabling allogeneic CAR‐T cell manufacturing. It preserves cell functionality and drives potent gene editing, CAR expression, and tumor cytotoxicity, while feature‐based analysis links these functional outcomes ...
Soohyun Jeon, Sein Kim, Hyun‐Young Kim, Duck Cho, Sungsu Park, Seohyun Kim, Aram J. Chung   +6 more
wiley   +1 more source