Trends in Peptide and Protein Sciences, 2019 The EpCAM (epithelial cell adhesion molecule) is a cell surface antigen over expressed in many types of epithelial cell cancers including colon, stomach, pancreas, lung, ovarian, and breast.
Seyyed ali Mohammadi Vadoud +3 more
doaj +1 more source
A Human-Derived Monoclonal Antibody Targeting Extracellular Connexin Domain Selectively Modulates Hemichannel Function [PDF]
, 2019 Connexin hemichannels, which are plasma membrane hexameric channels (connexons) composed of connexin protein protomers, have been implicated in a host of physiological processes and pathological conditions. A number of single point pathological mutations
Buratto, Damiano +12 more
core +1 more source
Nanobody based dual specific CARs [PDF]
, 2018 Recent clinical trials have shown that adoptive chimeric antigen receptor (CAR) T cell therapy is a very potent and possibly curative option in the treatment of B cell leukemias and lymphomas.
Abken, Hinrich +8 more
core +2 more sources
Human Vaccines & Immunotherapeutics, 2018 The aim of this study was to produce a humanized single chain antibody (scFv) as a potential improved product design to target EGFR (Epidermal Growth Factor Receptor) overexpressing cancer cells.
Arsham Banisadr +3 more
doaj +1 more source
A Cassette Vector System for the Rapid Cloning and Production of Bispecific Tetravalent Antibodies
Antibodies, 2012 Bivalent single chain (sc)Fv-Fc antibodies have been used for years as recombinant alternatives of natural immunoglobulins. We have extended this approach to the scFv-Fc-scFv antibody format to obtain tetravalent antigen binding and the possibility to ...
Stefanie Claudia Pohl +3 more
doaj +1 more source
Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv. [PDF]
PLoS ONE, 2017 CyDisCo is a system facilitating disulfide bond formation in recombinant proteins in the cytoplasm of Escherichia coli. Previously we screened for soluble expression of single chain antibody fragments (scFv) in the cytoplasm of E.
Anna Gąciarz, Lloyd W Ruddock
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Targeting fibroblast activation protein in tumor stroma with chimeric antigen receptor T cells can inhibit tumor growth and augment host immunity without severe toxicity. [PDF]
, 2014 The majority of chimeric antigen receptor (CAR) T-cell research has focused on attacking cancer cells. Here, we show that targeting the tumor-promoting, nontransformed stromal cells using CAR T cells may offer several advantages.
Albelda, Steven M. +13 more
core +2 more sources
Fusion Expression and Affinity Identification of Two scFvs Against CEA or CA19-9 in HEK293 Cells
Zhongliu Fangzhi Yanjiu, 2019 Objective To express two scFv-Fc fusion proteins of anti-CEA scFv-Fc and anti-CA19-9 scFv-Fc in HEK293 cells efficiently, and to determinate the affinity of two single chain antibodies. Methods Two scFv gene sequences obtained from GenBank were optimized
LU Wenbin +5 more
doaj +1 more source
Deletion of parasite immune modulatory sequences combined with immune activating signals enhances vaccine mediated protection against filarial nematodes [PDF]
, 2012 <p>Background: Filarial nematodes are tissue-dwelling parasites that can be killed by Th2-driven immune effectors, but that have evolved to withstand immune attack and establish chronic infections by suppressing host immunity. As a consequence, the
AB Fredriksen +63 more
core +4 more sources
Synthetic Customized scFv Libraries
, 2012 Libraries of antibody fragments displayed on filamentous phages have proved their value to generate human antibodies against virtually any target. We describe here a simple protocol to make large and diverse libraries based on a single or few frameworks.
Gautier, Robin, Pierre, Martineau
openaire +5 more sources