Results 31 to 40 of about 35,719 (245)
Frontiers in Immunology, 2021 Broadly neutralizing antibodies (bNAbs) are currently being assessed in clinical trials for their ability to prevent HIV infection. Single chain variable fragments (scFv) of bNAbs have advantages over full antibodies as their smaller size permits ...
Rebecca T. van Dorsten +8 more
doaj +1 more source
Engineering Peptide Linkers for scFv Immunosensors [PDF]
Analytical Chemistry, 2008 Using A10B single-chain fragment variable (scFv) as a model system, we demonstrated that the flexibility of scFv linker engineering can be combined with the inherent quick and adaptable characters of surface coupling chemistry (e.g., electrostatic, hydrogen bonding, or covalent attachment) to attach scFv to preformed functionalized self-assembled ...
Zhihong, Shen +4 more
openaire +2 more sources
scFv Antibody: Principles and Clinical Application [PDF]
Clinical and Developmental Immunology, 2012 To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments.
Zuhaida Asra Ahmad +5 more
openaire +3 more sources
Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update
Molecular Biotechnology, 2020 Single chain variable fragments (scFvs) are generated by joining together the variable heavy and light chain of a monoclonal antibody (mAb) via a peptide linker.
P. Satheeshkumar
semanticscholar +1 more source
Anti-tumoral potential of a human granulysin-based, CEA-targeted cytolytic immunotoxin
OncoImmunology, 2019 Granulysin is a protein present in the granules of human cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, with cytolytic activity against microbes and tumors.
Raquel Ibáñez-Pérez +11 more
doaj +1 more source
INCREASING OF THE EXPRESSION OF RECOMBINANT scFv-ANTIBODIES EFFICIENCY [PDF]
Biotechnologia Acta, 2017 Obtaining single-chain variable fragments (scFv) of recombinant antibodies in E. coli cells is often associated with numerous problems causing low yields or inactive conformation of the product.
O.V. Galkin +3 more
doaj +1 more source
Trends in Peptide and Protein Sciences, 2019 The EpCAM (epithelial cell adhesion molecule) is a cell surface antigen over expressed in many types of epithelial cell cancers including colon, stomach, pancreas, lung, ovarian, and breast.
Seyyed ali Mohammadi Vadoud +3 more
doaj +1 more source
Veterinary Research, 2020 Canine parvovirus (CPV) can cause acute and highly contagious bloody enteritis in dog. To obtain antibodies against CPV, hens were immunized with virus-like particles (VLP) of CPV-VP2.
Shikun Ge +5 more
semanticscholar +1 more source
Human Vaccines & Immunotherapeutics, 2018 The aim of this study was to produce a humanized single chain antibody (scFv) as a potential improved product design to target EGFR (Epidermal Growth Factor Receptor) overexpressing cancer cells.
Arsham Banisadr +3 more
doaj +1 more source
A Cassette Vector System for the Rapid Cloning and Production of Bispecific Tetravalent Antibodies
Antibodies, 2012 Bivalent single chain (sc)Fv-Fc antibodies have been used for years as recombinant alternatives of natural immunoglobulins. We have extended this approach to the scFv-Fc-scFv antibody format to obtain tetravalent antigen binding and the possibility to ...
Stefanie Claudia Pohl +3 more
doaj +1 more source