Results 31 to 40 of about 35,905 (251)

Anti-tumoral potential of a human granulysin-based, CEA-targeted cytolytic immunotoxin

OncoImmunology, 2019
Granulysin is a protein present in the granules of human cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, with cytolytic activity against microbes and tumors.
Raquel Ibáñez-Pérez, Patricia Guerrero-Ochoa, Sameer Al-Wasaby, Rocío Navarro, Antonio Tapia-Galisteo, Diego De Miguel, Oscar Gonzalo, Blanca Conde, Luis Martínez-Lostao, Ramón Hurtado-Guerrero, Laura Sanz, Alberto Anel   +11 more
doaj   +1 more source

Canine Parvovirus is diagnosed and neutralized by chicken IgY-scFv generated against the virus capsid protein

Veterinary Research, 2020
Canine parvovirus (CPV) can cause acute and highly contagious bloody enteritis in dog. To obtain antibodies against CPV, hens were immunized with virus-like particles (VLP) of CPV-VP2.
Shikun Ge, Longdi Xu, Ben Li, Fagang Zhong, Xiang Liu, Xiaoying Zhang   +5 more
semanticscholar   +1 more source

Effects of Cultivation Conditions on the Expression Level of Recombinant scFv Antibody against EpEX in Escherichia coli

Trends in Peptide and Protein Sciences, 2019
The EpCAM (epithelial cell adhesion molecule) is a cell surface antigen over expressed in many types of epithelial cell cancers including colon, stomach, pancreas, lung, ovarian, and breast.
Seyyed ali Mohammadi Vadoud, Atieh Hashemi, Malihe keramati, Farideh Rasouli Kery Bozorg   +3 more
doaj   +1 more source

Production of a germline-humanized cetuximab scFv and evaluation of its activity in recognizing EGFR- overexpressing cancer cells

Human Vaccines & Immunotherapeutics, 2018
The aim of this study was to produce a humanized single chain antibody (scFv) as a potential improved product design to target EGFR (Epidermal Growth Factor Receptor) overexpressing cancer cells.
Arsham Banisadr, Yaghoub Safdari, Anvarsadat Kianmehr, Mahdieh Pourafshar   +3 more
doaj   +1 more source

Fusion Expression and Affinity Identification of Two scFvs Against CEA or CA19-9 in HEK293 Cells

Zhongliu Fangzhi Yanjiu, 2019
Objective To express two scFv-Fc fusion proteins of anti-CEA scFv-Fc and anti-CA19-9 scFv-Fc in HEK293 cells efficiently, and to determinate the affinity of two single chain antibodies. Methods Two scFv gene sequences obtained from GenBank were optimized
LU Wenbin, GONG Xuechao, JIN Jianhua, HU Wenwei, WANG Yue, ZHANG Hua   +5 more
doaj   +1 more source

Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery

Molecular Medicine, 2016
Single-chain antibodies (scFvs), which contain only the variable domains of full-length antibodies, are relatively small molecules that can be used for selective drug delivery.
Yaghoub Safdari, Vahideh Ahmadzadeh, Masoumeh Khalili, Hossein Zarei Jaliani, Vahid Zarei, Vahid Erfani-Moghadam   +5 more
doaj   +1 more source

RGD4C peptide mediates anti-p21Ras scFv entry into tumor cells and produces an inhibitory effect on the human colon cancer cell line SW480

BMC Cancer, 2021
Background We prepared an anti-p21Ras scFv which could specifically bind with mutant and wild-type p21Ras. However, it cannot penetrate the cell membrane, which prevents it from binding to p21Ras in the cytoplasm.
Chen-Chen Huang, Fang-Rui Liu, Qiang Feng, Xin-Yan Pan, Shu-Ling Song, Ju-Lun Yang   +5 more
doaj   +1 more source

Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process

Microbial Cell Factories, 2019
BackgroundThe production of therapeutically active single chain variable fragment (scFv) antibody is still challenging in E. coli due to the aggregation propensity of recombinant protein into inclusion bodies (IBs).
A. Sarker, A. Rathore, R. Gupta
semanticscholar   +1 more source

Relative Contribution of Framework and CDR Regions in Antibody Variable Domains to Multimerisation of Fv- and scFv-Containing Bispecific Antibodies

Antibodies, 2018
Bispecific antibodies represent an emerging class of antibody drugs that are commonly generated by fusion of Fv or scFv antigen binding domains to IgG or Fab scaffolds.
Pallavi Bhatta, David P. Humphreys
doaj   +1 more source

Selection of novel human scFvs against cancer antigen IL1RAP by phage and yeast surface display technology

Biotechnology & Biotechnological Equipment, 2020
The objective of this study was isolation of humanized single-chain variable fragment (scFv) antibodies against IL1RAP, a promising cancer marker in leukemia and other tumors.
Shixin Duan, Yanrong Jia, Debao Xie, Shenglin Xiao, Cheng Zhou, Fanli Zeng   +5 more
doaj   +1 more source