Results 11 to 20 of about 35,905 (251)

Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv. [PDF]

PLoS ONE, 2017
CyDisCo is a system facilitating disulfide bond formation in recombinant proteins in the cytoplasm of Escherichia coli. Previously we screened for soluble expression of single chain antibody fragments (scFv) in the cytoplasm of E.
Anna Gąciarz, Lloyd W Ruddock
doaj   +2 more sources

A novel synthetic trivalent single chain variable fragment (tri-scFv) construction platform based on the SpyTag/SpyCatcher protein ligase system

BMC Biotechnology, 2018
Background Advances in antibody engineering provide strategies to construct recombinant antibody-like molecules with modified pharmacokinetic properties.
Md. Kausar Alam, Michelle Brabant, Raja Solomon Viswas, Kris Barreto, Humphrey Fonge, C. Ronald Geyer   +5 more
doaj   +2 more sources

Double attack strategy for leukemia using a pre-targeting bispecific antibody (CD20 Ab-mPEG scFv) and actively attracting PEGylated liposomal doxorubicin to enhance anti-tumor activity

Journal of Nanobiotechnology, 2021
Background Tumor-targeted nanoparticles hold great promise as new tools for therapy of liquid cancers. Furthermore, the therapeutic efficacy of nanoparticles can be improved by enhancing the cancer cellular internalization.
Kai-Wen Ho, I.-J.u Chen, Yi-An Cheng, Tzu-Yi Liao, En-Shuo Liu, Huei-Jen Chen, Yun-Chi Lu, Yu-Cheng Su, Steve R. Roffler, Bo-Cheng Huang, Hui-Ju Liu, Ming-Yii Huang, Chiao-Yun Chen, Tian-Lu Cheng   +13 more
doaj   +2 more sources

scFv Antibody: Principles and Clinical Application [PDF]

Clinical and Developmental Immunology, 2012
To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments.
Zuhaida Asra Ahmad, Swee Keong Yeap, Abdul Manaf Ali, Wan Yong Ho, Noorjahan Banu Alitheen, Muhajir Hamid   +5 more
openaire   +4 more sources

Target delivery of a PD-1-TREM2 scFv by CAR-T cells enhances anti-tumor efficacy in colorectal cancer

Molecular Cancer, 2023
Background Chimeric antigen receptor (CAR) -T cell therapy is an efficient therapeutic strategy for specific hematologic malignancies. However, positive outcomes of this novel therapy in treating solid tumors are curtailed by the immunosuppressive tumor ...
Jing Chen, Tianchuan Zhu, Guanmin Jiang, Qi Zeng, Zhijian Li, Xi Huang   +5 more
semanticscholar   +1 more source

Rapid, Point-of-Care scFv-SERS Assay for Femtogram Level Detection of SARS-CoV-2

ACS Sensors, 2022
Rapid, sensitive, on-site identification of SARS-CoV-2 infections is an important tool in the control and management of COVID-19. We have developed a surface-enhanced Raman scattering (SERS) immunoassay for highly sensitive detection of SARS-CoV-2 ...
D. Antoine, M. Mohammadi, Madison Vitt, Julia Marie Dickie, Sharmin Sultana Jyoti, M. Tilbury, P. A. Johnson, K. Wawrousek, J. Wall   +8 more
semanticscholar   +1 more source

Effect of non-repetitive linker on in vitro and in vivo properties of an anti-VEGF scFv

Scientific Reports, 2022
Single chain antibody fragments (scFvs) are favored in diagnostic and therapeutic fields thanks to their small size and the availability of various engineering approaches.
Merve Arslan, Murat Karadag, Ebru Onal, Emine Gelinci, G. Cakan-Akdogan, Sibel Kalyoncu   +5 more
semanticscholar   +1 more source

Generation and functional characterization of a single-chain variable fragment (scFv) of the anti-FGF2 3F12E7 monoclonal antibody

Scientific Reports, 2021
Single-chain variable fragments (scFvs) are small-sized artificial constructs composed of the immunoglobulin heavy and light chain variable regions connected by a peptide linker.
R. B. de Aguiar, Tábata de Almeida da Silva, B. Costa, M. Machado, R. Y. Yamada, Camila Braggion, K. R. Perez, M. Mori, Vitor Oliveira, Jane Zveiter de Moraes   +9 more
semanticscholar   +1 more source

Development of a SARS-CoV-2-specific biosensor for antigen detection using scFv-Fc fusion proteins

Biosensors & bioelectronics, 2020
Coronavirus disease 2019 (COVID-19) is a newly emerged human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

semanticscholar   +1 more source

Augmenting the Effectiveness of CAR-T Cells by Enhanced Self-Delivery of PD-1-Neutralizing scFv

Frontiers in Cell and Developmental Biology, 2020
Chimeric antigen receptor T (CAR-T) cell therapy is not satisfying in solid tumors. PD-1-mediated suppression greatly hinders CAR-T cells in the microenvironment. It has been shown that PD-1 blockade improves the effectiveness of CAR-T cells.
Y. Ping, Feng Li, S. Nan, Daiqun Zhang, Xiaojuan Shi, J. Shan, Yi Zhang   +6 more
semanticscholar   +1 more source