“Stapling” scFv for multispecific biotherapeutics of superior properties
mAbs, 2023 Single-chain fragment variable (scFv) domains play an important role in antibody-based therapeutic modalities, such as bispecifics, multispecifics and chimeric antigen receptor T cells or natural killer cells.
Lauren E. Boucher +29 more
doaj +3 more sources
Recombinant expression of monovalent and bivalent anti-TNT-antibodies: Evaluation of different expression systems [PDF]
Journal of the Serbian Chemical Society, 2008 Monoclonal 11B3 anti-TNT (trinitrotoluene) antibody was expressed as a monovalent and bivalent form using different prokaryotic and eukaryotic expression systems.
Simonović Mladen +3 more
doaj +1 more source
Trispecific T-cell engagers for dual tumor-targeting of colorectal cancer
OncoImmunology, 2022 Retargeting of T lymphocytes toward cancer cells by bispecific antibodies has demonstrated its therapeutic potential, with one such antibody approved for the treatment of acute lymphoblastic leukemia (blinatumomab) and several other in clinical trials ...
Antonio Tapia-Galisteo +15 more
doaj +1 more source
Novel Plantibodies Show Promise to Protect Citrus from Greening Disease
Journal of the American Society for Horticultural Science, 2021 Candidatus Liberibacter asiaticus (CLas), the bacteria responsible for citrus greening disease [huanglongbing (HLB)], has become a worldwide threat to citrus (Citrus sp.) production.
Joseph Krystel +3 more
doaj +1 more source
Successful Application of Whole Cell Panning for Isolation of Phage Antibody Fragments Specific to Differentiated Gastric Cancer Cells [PDF]
Advanced Pharmaceutical Bulletin, 2019 Purpose: Generation of antibodies which potentially discriminate between malignant and healthy cells is an important prerequisite for early diagnosis and treatment of gastric cancer (GC).
Sepideh Nikfarjam +5 more
doaj +1 more source
Radiolabeling, Quality Control and In Vivo Imaging of Multimodal Targeted Nanomedicines
Pharmaceutics, 2022 Following our previous study on the development of EGFR-targeted nanomedicine (NM-scFv) for the active delivery of siRNA in EGFR-positive cancers, this study focuses on the development and the quality control of a radiolabeling method to track it in in ...
Phuoc-Vinh Nguyen +7 more
doaj +1 more source
Chicken scFvs and bivalent scFv-CH fusions directed against HSP65 of Mycobacterium bovis [PDF]
Biologicals, 2010 Two chicken single-chain variable region antibody fragments (scFvs) that recognised the 65 kDa heat-shock protein (HSP65) of Mycobacterium bovis were selected from a large semi-synthetic phage displayed library. Both recognised HSP65 in indirect enzyme-linked immunosorbent assay (ELISA) and immunoblots and retained their activity during storage ...
Wemmer, Susan +4 more
openaire +3 more sources
Antibody-Mediated Resistance to Rhizomania Disease in Sugar Beet Hairy Roots
The Plant Pathology Journal, 2019 Agrobacterium rhizogenes–mediated transformation of sugar beet hairy roots expressing single-chain variable fragment (scFv) was exploited to evaluate the efficacy of four antibody-based constructs for interfering with the Beet necrotic yellow vein virus ...
M. Jafarzade +7 more
doaj +1 more source
Nonspecificity in a nonimmune human scFv repertoire [PDF]
mAbs, 2017 Efforts to develop effective antibody therapeutics are frequently hampered by issues such as aggregation and nonspecificity, often only detected in late stages of the development process. In this study, we used a high throughput cross-reactivity assay to select nonspecific clones from a naïve human repertoire scFv library displayed on the surface of ...
Kelly, Ryan L. +3 more
openaire +2 more sources
Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice. [PDF]
, 2018 Oxidized phospholipids (OxPL) are ubiquitous, are formed in many inflammatory tissues, including atherosclerotic lesions, and frequently mediate proinflammatory changes 1 .
Binder, Christoph J +21 more
core +1 more source