Results 11 to 20 of about 23,836 (161)
Heliyon, 2023 Heart failure has a high global prevalence, with symptoms such as breathlessness, fatigue, and swelling. Early detection is crucial, as the condition worsens over time and can be fatal. This study identified the single-chain variable fragment (scFv) that
Sureeporn Wongjard +7 more
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Frontiers in Immunology, 2021 Broadly neutralizing antibodies (bNAbs) are currently being assessed in clinical trials for their ability to prevent HIV infection. Single chain variable fragments (scFv) of bNAbs have advantages over full antibodies as their smaller size permits ...
Rebecca T. van Dorsten +8 more
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Anti-tumoral potential of a human granulysin-based, CEA-targeted cytolytic immunotoxin
OncoImmunology, 2019 Granulysin is a protein present in the granules of human cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, with cytolytic activity against microbes and tumors.
Raquel Ibáñez-Pérez +11 more
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INCREASING OF THE EXPRESSION OF RECOMBINANT scFv-ANTIBODIES EFFICIENCY [PDF]
Biotechnologia Acta, 2017 Obtaining single-chain variable fragments (scFv) of recombinant antibodies in E. coli cells is often associated with numerous problems causing low yields or inactive conformation of the product.
O.V. Galkin +3 more
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Trends in Peptide and Protein Sciences, 2019 The EpCAM (epithelial cell adhesion molecule) is a cell surface antigen over expressed in many types of epithelial cell cancers including colon, stomach, pancreas, lung, ovarian, and breast.
Seyyed ali Mohammadi Vadoud +3 more
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Human Vaccines & Immunotherapeutics, 2018 The aim of this study was to produce a humanized single chain antibody (scFv) as a potential improved product design to target EGFR (Epidermal Growth Factor Receptor) overexpressing cancer cells.
Arsham Banisadr +3 more
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A Cassette Vector System for the Rapid Cloning and Production of Bispecific Tetravalent Antibodies
Antibodies, 2012 Bivalent single chain (sc)Fv-Fc antibodies have been used for years as recombinant alternatives of natural immunoglobulins. We have extended this approach to the scFv-Fc-scFv antibody format to obtain tetravalent antigen binding and the possibility to ...
Stefanie Claudia Pohl +3 more
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Fusion Expression and Affinity Identification of Two scFvs Against CEA or CA19-9 in HEK293 Cells
Zhongliu Fangzhi Yanjiu, 2019 Objective To express two scFv-Fc fusion proteins of anti-CEA scFv-Fc and anti-CA19-9 scFv-Fc in HEK293 cells efficiently, and to determinate the affinity of two single chain antibodies. Methods Two scFv gene sequences obtained from GenBank were optimized
LU Wenbin +5 more
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Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv. [PDF]
PLoS ONE, 2017 CyDisCo is a system facilitating disulfide bond formation in recombinant proteins in the cytoplasm of Escherichia coli. Previously we screened for soluble expression of single chain antibody fragments (scFv) in the cytoplasm of E.
Anna Gąciarz, Lloyd W Ruddock
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Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery
Molecular Medicine, 2016 Single-chain antibodies (scFvs), which contain only the variable domains of full-length antibodies, are relatively small molecules that can be used for selective drug delivery.
Yaghoub Safdari +5 more
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