Results 1 to 10 of about 44,698 (301)

Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery [PDF]

Molecular Medicine, 2016
Single-chain antibodies (scFvs), which contain only the variable domains of full-length antibodies, are relatively small molecules that can be used for selective drug delivery.
Yaghoub Safdari, Vahideh Ahmadzadeh, Masoumeh Khalili, Hossein Zarei Jaliani, Vahid Zarei, Vahid Erfani-Moghadam   +5 more
doaj   +3 more sources

Phase I clinical study of the recombinant antibody toxin scFv(FRP5)-ETA specific for the ErbB2/HER2 receptor in patients with advanced solid malignomas [PDF]

, 2005
Introduction: ScFv(FRP5)-ETA is a recombinant antibody toxin with binding specificity for ErbB2 (HER2). It consists of an N-terminal single-chain antibody fragment (scFv), genetically linked to truncated Pseudomonas exotoxin A (ETA).
Gunter von Minckwitz, Sebastian Harder, Sascha Hövelmann, Elke Jäger, Salah-Eddin Al-Batran, Sibylle Loibl, Akin Atmaca, Christian Cimpoiasu, Antje Neumann, Aklil Abera, Alexander Knuth, Manfred Kaufmann, Dirk Jäger, Alexander B Maurer, Winfried S Wels, MA Olayioye, Y Yarden, J Mendelsohn, DJ Slamon, CL Vogel, I Pastan, W Wels, RJ Kreitman, W Wels, W Wels, IM Harwerth, BH Iglewski, A Keppler-Hafkemeyer, M Schmidt, A Spyridonidis, M Azemar, L Wang, U Altenschmidt, M Maurer-Gebhard, JK Batra, Y Reiter, LH Pai-Scherf, M Azemar, WMA, M Maurer-Gebhard, FDA, J Schumann, M Onda, NJ Olsen, YL Yip, B Gerstmayer, DL Keefe, HS Cho, RJ Kreitman, RJ Kreitman   +49 more
core   +3 more sources

Selection, alkaline phosphatase fusion, and application of single-chain variable fragment (scFv) specific to NT-proBNP as electrochemical immunosensor for heart failure [PDF]

Heliyon, 2023
Heart failure has a high global prevalence, with symptoms such as breathlessness, fatigue, and swelling. Early detection is crucial, as the condition worsens over time and can be fatal. This study identified the single-chain variable fragment (scFv) that
Sureeporn Wongjard, Pongsakorn Aiemderm, Kanchana Monkhang, Kittitat Jaengwang, Lueacha Tabtimmai, Charoenkwan Kraiya, Kiattawee Choowongkomon, Napachanok Mongkoldhumrongkul Swainson   +7 more
doaj   +2 more sources

Development of a recombinant biosimilar single-chain variable fragment antibody targeting human estrogen receptor α36 [PDF]

Research in Pharmaceutical Sciences
Background and purpose: The estrogen receptor alpha-36 (ER-α36) is an alternative splice variant of classical ER-α66 and is abundantly present in both ER-α66-positive and ER-α66-negative breast tumor cells.
Soodabeh Shafiee, Sedighe Kolivand, Neda Jalili, Mohammad Abedini, Mahboubeh Navabi, Yeganeh Talebkhan, Rezvan Esmaeili, Mohadeseh Haji Abdolvahab   +7 more
doaj   +2 more sources

Development of a Komagataella phaffii-based expression system for high-activity anti-SARS-CoV-2 neutralizing antibodies [PDF]

AMB Express
The growing need for rapid and cost-effective antibody production highlights the potential of Komagataella phaffii (syn. Pichia pastoris) as an alternative to mammalian systems.
Xueqin Liu, Qiaoli Lang, Nan Huang, Xiaoyan You, Yuchun Ding, Meng Wu, Liangpeng Ge, Xi Yang   +7 more
doaj   +2 more sources

Engineering production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp

Frontiers in Cellular and Infection Microbiology, 2013
Single-chain variable fragment (scFv) is a class of engineered antibodies generated by the fusion of the heavy (VH) and light chains (VL) of immunoglobulins through a short polypeptide linker.
Swaminath Srinivas, Shihua Wang
exaly   +3 more sources

Preparation and Directed Evolution of Anti-Ciprofloxacin ScFv for Immunoassay in Animal-Derived Food [PDF]

Foods, 2021
Jian-Ming Fan
exaly   +2 more sources

Development of a single-chain fragment variable fused-mutant HALT-1 recombinant immunotoxin against G12V mutated KRAS colorectal cancer cells [PDF]

PeerJ, 2021
Background KRAS oncogenes harboring codon G12 and G13 substitutions are considered gatekeeper mutations which drive oncogenesis in many cancers.
Michelle Yee Mun Teo, Jeremy Jeack Ceen Ng, Jung Yin Fong, Jung Shan Hwang, Adelene Ai-Lian Song, Renee Lay Hong Lim, Lionel Lian Aun In   +6 more
doaj   +2 more sources

Kinetic Characterisation of a Single Chain Antibody against the Hormone Abscisic Acid: Comparison with Its Parental Monoclonal [PDF]

, 2016
A single-chain Fv fragment antibody (scFv) specific for the plant hormone abscisic acid (ABA) has been expressed in the bacterium Escherichia coli as a fusion protein.
A Hayhurst, A Sadanandom, A Wörn, AM Jones, Andrew J. Thompson, Andrew Marsh, B Kazemier, D Missiakas, DG Myszka, DG Myszka, DM Hoover, Ellen R Goldman, George O. Badescu, GM Banowetz, J Messens, J Phillips, J Wigger, LLH Christensen, M Malmqvist, MK Walker-Simmons, O Artsaenko, O Artsaenko, P Jurado, P Martineau, PB Luppa, Q Li, R Mertens, R Raag, R Waadt, Richard M. Napier, S Shaki-Loewensteina, SA Quarrie, SA Quarrie, SM Kipriyanova, Timothy R. Smith, TR Smith, UL Jönsson, WFM Stöcklein, Y-W Li   +38 more
core   +16 more sources

Development of alkaline phosphatase-scFv and its use for one-step enzyme-linked immunosorbent assay for His-tagged protein detection

Open Life Sciences, 2022
A histidine (His)-tag is composed of six His residues and typically exerts little influence on the structure and solubility of expressed recombinant fusion proteins.
He Shuzhen, Xu Ruixian, Yi Huashan, Chen Zhixin, Chen Congjie, Li Qiang, Han Qinqin, Xia Xueshan, Song Yuzhu, Xu Junwei, Zhang Jinyang   +10 more
doaj   +1 more source