Results 41 to 50 of about 35,719 (245)
Fusion Expression and Affinity Identification of Two scFvs Against CEA or CA19-9 in HEK293 Cells
Zhongliu Fangzhi Yanjiu, 2019 Objective To express two scFv-Fc fusion proteins of anti-CEA scFv-Fc and anti-CA19-9 scFv-Fc in HEK293 cells efficiently, and to determinate the affinity of two single chain antibodies. Methods Two scFv gene sequences obtained from GenBank were optimized
LU Wenbin +5 more
doaj +1 more source
Synthetic Customized scFv Libraries
, 2012 Libraries of antibody fragments displayed on filamentous phages have proved their value to generate human antibodies against virtually any target. We describe here a simple protocol to make large and diverse libraries based on a single or few frameworks.
Gautier, Robin, Pierre, Martineau
openaire +5 more sources
Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery
Molecular Medicine, 2016 Single-chain antibodies (scFvs), which contain only the variable domains of full-length antibodies, are relatively small molecules that can be used for selective drug delivery.
Yaghoub Safdari +5 more
doaj +1 more source
BMC Cancer, 2021 Background We prepared an anti-p21Ras scFv which could specifically bind with mutant and wild-type p21Ras. However, it cannot penetrate the cell membrane, which prevents it from binding to p21Ras in the cytoplasm.
Chen-Chen Huang +5 more
doaj +1 more source
Microbial Cell Factories, 2019 BackgroundThe production of therapeutically active single chain variable fragment (scFv) antibody is still challenging in E. coli due to the aggregation propensity of recombinant protein into inclusion bodies (IBs).
A. Sarker, A. Rathore, R. Gupta
semanticscholar +1 more source
Food and Agricultural Immunology, 2018 Amantadine (AMA) is an illegally used antiviral drug in the poultry industry, it is necessary to establish a fast, accurate and time-saving detection method for poultry food. The antibody-based immunoassay can achieve fast and accurate requirements.
Sanlei Xie +5 more
doaj +1 more source
Antibodies, 2018 Bispecific antibodies represent an emerging class of antibody drugs that are commonly generated by fusion of Fv or scFv antigen binding domains to IgG or Fab scaffolds.
Pallavi Bhatta, David P. Humphreys
doaj +1 more source
Biotechnology & Biotechnological Equipment, 2020 The objective of this study was isolation of humanized single-chain variable fragment (scFv) antibodies against IL1RAP, a promising cancer marker in leukemia and other tumors.
Shixin Duan +5 more
doaj +1 more source
Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells [PDF]
Advanced Pharmaceutical Bulletin, 2017 Purpose: More than half of the diagnostic and therapeutic recombinant protein production depends on mammalian-based expression system. However, the generation of recombinant antibodies remains a challenge in mammalian cells due to the disulfide bond ...
Mohammad Tasyriq Che Omar
doaj +1 more source
A T-cell–engaging B7-H4/CD3-bispecific Fab-scFv Antibody Targets Human Breast Cancer
Clinical Cancer Research, 2019 Purpose: The B7 homolog 4 (B7-H4, VTCN1) is an immune checkpoint molecule that negatively regulates immune responses and is known to be overexpressed in many human cancers.
A. Iizuka +11 more
semanticscholar +1 more source