Results 41 to 50 of about 35,905 (251)
Food and Agricultural Immunology, 2018 Amantadine (AMA) is an illegally used antiviral drug in the poultry industry, it is necessary to establish a fast, accurate and time-saving detection method for poultry food. The antibody-based immunoassay can achieve fast and accurate requirements.
Sanlei Xie +5 more
doaj +1 more source
Purification of a Novel Anti-VEGFR2 Single Chain Antibody Fragmentand Evaluation of Binding Affinity by Surface Plasmon Resonance [PDF]
Advanced Pharmaceutical Bulletin, 2019 Purpose: The single-chain variable fragment (scFv) domain of antibodies is now considered asone of the therapeutic tools that can be produced by phage display technology (PDT).
Shirafkan Kordi +4 more
doaj +1 more source
Synthetic Customized scFv Libraries
, 2012 Libraries of antibody fragments displayed on filamentous phages have proved their value to generate human antibodies against virtually any target. We describe here a simple protocol to make large and diverse libraries based on a single or few frameworks.
Gautier, Robin, Pierre, Martineau
openaire +5 more sources
Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells [PDF]
Advanced Pharmaceutical Bulletin, 2017 Purpose: More than half of the diagnostic and therapeutic recombinant protein production depends on mammalian-based expression system. However, the generation of recombinant antibodies remains a challenge in mammalian cells due to the disulfide bond ...
Mohammad Tasyriq Che Omar
doaj +1 more source
A strategy for adenovirus vector targeting with a secreted single chain antibody. [PDF]
PLoS ONE, 2009 BACKGROUND:Successful gene therapy will require targeted delivery vectors capable of self-directed localization. In this regard, the use of antibodies or single chain antibody fragments (scFv) in conjunction with adenovirus (Ad) vectors remains an ...
Joel N Glasgow +3 more
doaj +1 more source
Biomolecules, 2023 Although single-chain variable fragment (scFv) is recognized as a highly versatile scaffold of recombinant antibody fragment molecules, its overexpression in Escherichia coli often leads to the formation of inclusion bodies.
Kyu Tae Byun +8 more
doaj +1 more source
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
Encoded Cell‐Material Interactions to Reroute Cytokine Signaling for Regenerative Medicine
Advanced Healthcare Materials, EarlyView.We present native MATRIX (Material Activated To Regulate Inducible gene eXpression), a co‐engineered material‐cell platform. Surfaces functionalized with antibodies for endogenous soluble ligands (e.g., IL‐1β or IL‐6) capture and immobilize ligands for presentation to a cognate, engineered synthetic Notch receptor, releasing a transcription factor (TF)
Zachary M. Eidman +7 more
wiley +1 more source
Theranostics, 2019 Investigation of targeting inhibitors of Aβ aggregation, heme-Aβ peroxidase-like activity and efficient detectors of Aβ aggregation, are of therapeutic value and diagnostics significance for the treatment of Alzheimer's disease (AD).
Dongni Liu +10 more
semanticscholar +1 more source
Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C [PDF]
The Ukrainian Biochemical Journal, 2015 The aim of this work was to obtain the recombinant single chain variable fragments of antibodies (scFv) against human protein C, the key component of blood anticoagulation system. For this purpose a peptide that mimics a Pro144-Leu155 sequence of protein
O. S. Oliinyk +5 more
doaj +1 more source