Results 41 to 50 of about 44,698 (301)

RGD4C peptide mediates anti-p21Ras scFv entry into tumor cells and produces an inhibitory effect on the human colon cancer cell line SW480

BMC Cancer, 2021
Background We prepared an anti-p21Ras scFv which could specifically bind with mutant and wild-type p21Ras. However, it cannot penetrate the cell membrane, which prevents it from binding to p21Ras in the cytoplasm.
Chen-Chen Huang, Fang-Rui Liu, Qiang Feng, Xin-Yan Pan, Shu-Ling Song, Ju-Lun Yang   +5 more
doaj   +1 more source

Positron emission tomography imaging of endometrial cancer using engineered anti-EMP2 antibody fragments. [PDF]

, 2012
PurposeAs imaging of the cell surface tetraspan protein epithelial membrane protein-2 (EMP2) expression in malignant tumors may provide important prognostic and predictive diagnostic information, the goal of this study is to determine if antibody ...
Braun, Jonathan, Brewer, Sarah, Fu, Maoyong, Gordon, Lynn K, Olafsen, Tove, Said, Jonathan, Wadehra, Madhuri, Wu, Anna M   +7 more
core   +3 more sources

Safety, tumor trafficking and immunogenicity of chimeric antigen receptor (CAR)-T cells specific for TAG-72 in colorectal cancer. [PDF]

, 2017
BackgroundT cells engineered to express chimeric antigen receptors (CARs) have established efficacy in the treatment of B-cell malignancies, but their relevance in solid tumors remains undefined.
A Molinolo, A Stasi Di, A Thor, B Fisher, BA Irving, BL Levine, C Romeo, CC Kloss, CY Wu, D Moritz, DL Porter, DW Lee, F Guadagni, HM Finney, I Blanco, I Stancovski, J Scholler, JD Wolchok, JL Murray, JN Kochenderfer, JW Greiner, KD Griffith, L Naldini, M Hegde, M Kalos, ME Dudley, MH Finer, MH Kershaw, ML Davila, MR Lander, N Gonzales, NP Restifo, P Hwu, RA Morgan, RE Walker, RF Meredith, RP McGuinness, RT Mitsuyasu, S Guedan, SD Patel, SG Deeks, SL Maude, SV Kashmiri   +42 more
core   +1 more source

A novel variable antibody fragment dimerized by the dHLX peptide with enhanced affinity against amantadine compared to its corresponding scFv antibody

Food and Agricultural Immunology, 2018
Amantadine (AMA) is an illegally used antiviral drug in the poultry industry, it is necessary to establish a fast, accurate and time-saving detection method for poultry food. The antibody-based immunoassay can achieve fast and accurate requirements.
Sanlei Xie, Kai Wen, Tao Peng, Jianyi Wang, Kai Yao, Haiyang Jiang   +5 more
doaj   +1 more source

Selection of novel human scFvs against cancer antigen IL1RAP by phage and yeast surface display technology

Biotechnology & Biotechnological Equipment, 2020
The objective of this study was isolation of humanized single-chain variable fragment (scFv) antibodies against IL1RAP, a promising cancer marker in leukemia and other tumors.
Shixin Duan, Yanrong Jia, Debao Xie, Shenglin Xiao, Cheng Zhou, Fanli Zeng   +5 more
doaj   +1 more source

Relative Contribution of Framework and CDR Regions in Antibody Variable Domains to Multimerisation of Fv- and scFv-Containing Bispecific Antibodies

Antibodies, 2018
Bispecific antibodies represent an emerging class of antibody drugs that are commonly generated by fusion of Fv or scFv antigen binding domains to IgG or Fab scaffolds.
Pallavi Bhatta, David P. Humphreys
doaj   +1 more source

Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells [PDF]

Advanced Pharmaceutical Bulletin, 2017
Purpose: More than half of the diagnostic and therapeutic recombinant protein production depends on mammalian-based expression system. However, the generation of recombinant antibodies remains a challenge in mammalian cells due to the disulfide bond ...
Mohammad Tasyriq Che Omar
doaj   +1 more source

Intrabodies Binding the Proline-Rich Domains of Mutant Huntingtin Increase Its Turnover and Reduce Neurotoxicity [PDF]

, 2008
Although expanded polyglutamine (polyQ) repeats are inherently toxic, causing at least nine neurodegenerative diseases, the protein context determines which neurons are affected.
Bugg, Charles W., Dunn, Denise E., Khoshnan, Ali, Lo, Donald C., Patterson, Paul H., Southwell, Amber L.   +5 more
core   +2 more sources

Purification of a Novel Anti-VEGFR2 Single Chain Antibody Fragmentand Evaluation of Binding Affinity by Surface Plasmon Resonance [PDF]

Advanced Pharmaceutical Bulletin, 2019
Purpose: The single-chain variable fragment (scFv) domain of antibodies is now considered asone of the therapeutic tools that can be produced by phage display technology (PDT).
Shirafkan Kordi, Mohammad Rahmati-Yamchi, Mehdi Asghari Vostakolaei, Abolfazl Barzegari, Jalal Abdolalizadeh   +4 more
doaj   +1 more source

Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain. [PDF]

, 2018
DNA methylation is an important epigenetic modification involved in gene regulation and transposable element silencing. Changes in DNA methylation can be heritable and, thus, can lead to the formation of stable epialleles. A well-characterized example of
Gallego-Bartolomé, Javier, Gardiner, Jason, Ghoshal, Basudev, Jacobsen, Steven E, Kuo, Hsuan Yu, Liu, Wanlu, Papikian, Ashot, Segal, David J, Zhao, Jenny Miao-Chi   +8 more
core   +2 more sources