Results 21 to 30 of about 35,719 (245)

Radiolabeling, Quality Control and In Vivo Imaging of Multimodal Targeted Nanomedicines

Pharmaceutics, 2022
Following our previous study on the development of EGFR-targeted nanomedicine (NM-scFv) for the active delivery of siRNA in EGFR-positive cancers, this study focuses on the development and the quality control of a radiolabeling method to track it in in ...
Phuoc-Vinh Nguyen, Emilie Allard-Vannier, Nicolas Aubrey, Christine Labrugère-Sarroste, Igor Chourpa, Julien Sobilo, Alain Le Pape, Katel Hervé-Aubert   +7 more
doaj   +1 more source

Folding Nuclei of the scFv Fragment of an Antibody [PDF]

Biochemistry, 1996
The folding kinetics of the variable domains of the phosphorylcholine-binding antibody McPC603, combined into a scFv fragment [VH-(Gly4Ser)3-VL], were investigated by the use of fluorescence spectroscopy, nuclear magnetic resonance (NMR), and mass spectrometry (MS).
Freund C, Honegger A, Hunziker P, Holak TA, Plückthun A   +4 more
openaire   +3 more sources

Antibody-Mediated Resistance to Rhizomania Disease in Sugar Beet Hairy Roots

The Plant Pathology Journal, 2019
Agrobacterium rhizogenes–mediated transformation of sugar beet hairy roots expressing single-chain variable fragment (scFv) was exploited to evaluate the efficacy of four antibody-based constructs for interfering with the Beet necrotic yellow vein virus ...
M. Jafarzade, M. Ramezani, F. Hedayati, Z. Mokhtarzade, B. Zare, M. S. Sabet, P. Norouzi, M. A. Malboobi   +7 more
doaj   +1 more source

Intratumoral Delivery of a PD-1–Blocking scFv Encoded in Oncolytic HSV-1 Promotes Antitumor Immunity and Synergizes with TIGIT Blockade

Cancer immunology research, 2020
Oncolytic virus activity is often limited by an immunosuppressive tumor microenvironment. The antitumor efficacy of oncolytic herpes simplex virus is improved by delivering a single-chain variable fragment specific for PD-1.
Chaolong Lin, Wenfeng Ren, Yong Luo, Shaopeng Li, Yating Chang, Lu Li, D. Xiong, Xiaoxuan Huang, Zilong Xu, Zeng Yu, Yingbing Wang, Jun Zhang, Chenghao Huang, N. Xia   +13 more
semanticscholar   +1 more source

Chicken scFvs and bivalent scFv-CH fusions directed against HSP65 of Mycobacterium bovis [PDF]

Biologicals, 2010
Two chicken single-chain variable region antibody fragments (scFvs) that recognised the 65 kDa heat-shock protein (HSP65) of Mycobacterium bovis were selected from a large semi-synthetic phage displayed library. Both recognised HSP65 in indirect enzyme-linked immunosorbent assay (ELISA) and immunoblots and retained their activity during storage ...
Wemmer, Susan, Mashau, Cordelia, Fehrsen, Jeanni, Van Wyngaardt, Wouter, Du Plessis, Dion H.   +4 more
openaire   +3 more sources

Targeted delivery of a PD-1-blocking scFv by CAR-T cells enhances anti-tumor efficacy in vivo

Nature Biotechnology, 2018
The efficacy of chimeric antigen receptor (CAR) T cell therapy against poorly responding tumors can be enhanced by administering the cells in combination with immune checkpoint blockade inhibitors.
S. Rafiq, O. Yeku, H. J. Jackson, Terence J. Purdon, Dayenne G van Leeuwen, Dylan J Drakes, Mei Song, Matthew M. Miele, Zhuoning Li, Pei Wang, Su Yan, Jingyi Xiang, Xiaojing Ma, V. Seshan, R. Hendrickson, Cheng Liu, R. Brentjens   +16 more
semanticscholar   +1 more source

Nonspecificity in a nonimmune human scFv repertoire [PDF]

mAbs, 2017
Efforts to develop effective antibody therapeutics are frequently hampered by issues such as aggregation and nonspecificity, often only detected in late stages of the development process. In this study, we used a high throughput cross-reactivity assay to select nonspecific clones from a naïve human repertoire scFv library displayed on the surface of ...
Kelly, Ryan L., Zhao, Jessie, Le, Doris, Wittrup, K. Dane   +3 more
openaire   +2 more sources

Augmenting the Effectiveness of CAR-T Cells by Enhanced Self-Delivery of PD-1-Neutralizing scFv

Frontiers in Cell and Developmental Biology, 2020
Chimeric antigen receptor T (CAR-T) cell therapy is not satisfying in solid tumors. PD-1-mediated suppression greatly hinders CAR-T cells in the microenvironment. It has been shown that PD-1 blockade improves the effectiveness of CAR-T cells.
Y. Ping, Feng Li, S. Nan, Daiqun Zhang, Xiaojuan Shi, J. Shan, Yi Zhang   +6 more
semanticscholar   +1 more source

Toward low-cost affinity reagents: lyophilized yeast-scFv probes specific for pathogen antigens. [PDF]

PLoS ONE, 2012
The generation of affinity reagents, usually monoclonal antibodies, remains a critical bottleneck in biomedical research and diagnostic test development.
Sean A Gray, Kris M Weigel, Ibne K M Ali, Annie A Lakey, Jeremy Capalungan, Gonzalo J Domingo, Gerard A Cangelosi   +6 more
doaj   +1 more source

Selection, alkaline phosphatase fusion, and application of single-chain variable fragment (scFv) specific to NT-proBNP as electrochemical immunosensor for heart failure

Heliyon, 2023
Heart failure has a high global prevalence, with symptoms such as breathlessness, fatigue, and swelling. Early detection is crucial, as the condition worsens over time and can be fatal. This study identified the single-chain variable fragment (scFv) that
Sureeporn Wongjard, Pongsakorn Aiemderm, Kanchana Monkhang, Kittitat Jaengwang, Lueacha Tabtimmai, Charoenkwan Kraiya, Kiattawee Choowongkomon, Napachanok Mongkoldhumrongkul Swainson   +7 more
doaj   +1 more source