Results 21 to 30 of about 44,698 (301)

Folding Nuclei of the scFv Fragment of an Antibody [PDF]

Biochemistry, 1996
The folding kinetics of the variable domains of the phosphorylcholine-binding antibody McPC603, combined into a scFv fragment [VH-(Gly4Ser)3-VL], were investigated by the use of fluorescence spectroscopy, nuclear magnetic resonance (NMR), and mass spectrometry (MS).
Freund C, Honegger A, Hunziker P, Holak TA, Plückthun A   +4 more
openaire   +3 more sources

Expression of an anti-CD33 single-chain antibody by Pichia pastoris [PDF]

, 2005
CD33 is a cell surface glycoprotein expressed on cells of myelomonocytic lineage, leukaemic cells, but not haematopoietic stem cells. By virtue of its expression pattern, CD33 has become a popular target for new immunotherapeutic approaches to treat ...
Emberson, Louise M., Trivett, Amanda J., Blower, Philip J., Nicholls, Peter J.   +3 more
core   +1 more source

Toward low-cost affinity reagents: lyophilized yeast-scFv probes specific for pathogen antigens. [PDF]

PLoS ONE, 2012
The generation of affinity reagents, usually monoclonal antibodies, remains a critical bottleneck in biomedical research and diagnostic test development.
Sean A Gray, Kris M Weigel, Ibne K M Ali, Annie A Lakey, Jeremy Capalungan, Gonzalo J Domingo, Gerard A Cangelosi   +6 more
doaj   +1 more source

Selection of functional human antibodies from retroviral display libraries [PDF]

, 2004
Antibody library technology represents a powerful tool for the discovery and design of antibodies with high affinity and specificity for their targets.
Urban, Johannes H., Schneider, Richard M., Compte, Marta, Finger, Carsten, Cichutek, Klaus, Álvarez-Vallina, Luis, Buchholz, Christian   +6 more
core   +2 more sources

Combinations of Single Chain Variable Fragments From HIV Broadly Neutralizing Antibodies Demonstrate High Potency and Breadth

Frontiers in Immunology, 2021
Broadly neutralizing antibodies (bNAbs) are currently being assessed in clinical trials for their ability to prevent HIV infection. Single chain variable fragments (scFv) of bNAbs have advantages over full antibodies as their smaller size permits ...
Rebecca T. van Dorsten, Rebecca T. van Dorsten, Kshitij Wagh, Penny L. Moore, Penny L. Moore, Penny L. Moore, Lynn Morris, Lynn Morris, Lynn Morris   +8 more
doaj   +1 more source

Production, characterization, and antigen specificity of recombinant 62-71-3, a candidate monoclonal antibody for rabies prophylaxis in humans [PDF]

, 2013
Rabies kills many people throughout the developing world every year. The murine monoclonal antibody (mAb) 62-71-3 was recently identified for its potential application in rabies postexposure prophylaxis (PEP).
Anthony R. Fooks, Ashley C. Banyard, Bardor M., Benmansour A., Bianca Bulmer‐Thomas, Constantine D. G., Craig Dolleweerd, David Selden, Dietzschold B., Dietzschold B., Dietzschold B., Edward Wright, Fooks A. R., Friedrich Altmann, Gomord V., Hampson K., Hultberg A., Julian K.‐C. Ma, Knobel D. L., Lafay F., Lafon M., Leonard Both, Luo T. R., Ma J. K., Manning S. E., Mansfield K. L., McCormick A. A., Muhamuda K., Müller T., Prehaud C., Satpathy D. M., Seif I., Shantavasinkul P., Sudarshan M. K., Tacket C. O., Tekoah Y., Van Ree R., Warrell M., Wertheim H. F. L., World Health Organization, World Health Organization, Wright E., Zeitlin L.   +42 more
core   +1 more source

Engineering Peptide Linkers for scFv Immunosensors [PDF]

Analytical Chemistry, 2008
Using A10B single-chain fragment variable (scFv) as a model system, we demonstrated that the flexibility of scFv linker engineering can be combined with the inherent quick and adaptable characters of surface coupling chemistry (e.g., electrostatic, hydrogen bonding, or covalent attachment) to attach scFv to preformed functionalized self-assembled ...
Zhihong, Shen, Heping, Yan, Ying, Zhang, Raymond L, Mernaugh, Xiangqun, Zeng   +4 more
openaire   +2 more sources

scFv Antibody: Principles and Clinical Application [PDF]

Clinical and Developmental Immunology, 2012
To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments.
Zuhaida Asra Ahmad, Swee Keong Yeap, Abdul Manaf Ali, Wan Yong Ho, Noorjahan Banu Alitheen, Muhajir Hamid   +5 more
openaire   +3 more sources

Anti-tumoral potential of a human granulysin-based, CEA-targeted cytolytic immunotoxin

OncoImmunology, 2019
Granulysin is a protein present in the granules of human cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, with cytolytic activity against microbes and tumors.
Raquel Ibáñez-Pérez, Patricia Guerrero-Ochoa, Sameer Al-Wasaby, Rocío Navarro, Antonio Tapia-Galisteo, Diego De Miguel, Oscar Gonzalo, Blanca Conde, Luis Martínez-Lostao, Ramón Hurtado-Guerrero, Laura Sanz, Alberto Anel   +11 more
doaj   +1 more source

INCREASING OF THE EXPRESSION OF RECOMBINANT scFv-ANTIBODIES EFFICIENCY [PDF]

Biotechnologia Acta, 2017
Obtaining single-chain variable fragments (scFv) of recombinant antibodies in E. coli cells is often associated with numerous problems causing low yields or inactive conformation of the product.
O.V. Galkin, T.O. Chudina, A.A. Siromolot, O.S. Oliynyk   +3 more
doaj   +1 more source