Results 21 to 30 of about 23,836 (161)
BMC Cancer, 2021 Background We prepared an anti-p21Ras scFv which could specifically bind with mutant and wild-type p21Ras. However, it cannot penetrate the cell membrane, which prevents it from binding to p21Ras in the cytoplasm.
Chen-Chen Huang +5 more
doaj +1 more source
Food and Agricultural Immunology, 2018 Amantadine (AMA) is an illegally used antiviral drug in the poultry industry, it is necessary to establish a fast, accurate and time-saving detection method for poultry food. The antibody-based immunoassay can achieve fast and accurate requirements.
Sanlei Xie +5 more
doaj +1 more source
Antibodies, 2018 Bispecific antibodies represent an emerging class of antibody drugs that are commonly generated by fusion of Fv or scFv antigen binding domains to IgG or Fab scaffolds.
Pallavi Bhatta, David P. Humphreys
doaj +1 more source
Biotechnology & Biotechnological Equipment, 2020 The objective of this study was isolation of humanized single-chain variable fragment (scFv) antibodies against IL1RAP, a promising cancer marker in leukemia and other tumors.
Shixin Duan +5 more
doaj +1 more source
Purification of a Novel Anti-VEGFR2 Single Chain Antibody Fragmentand Evaluation of Binding Affinity by Surface Plasmon Resonance [PDF]
Advanced Pharmaceutical Bulletin, 2019 Purpose: The single-chain variable fragment (scFv) domain of antibodies is now considered asone of the therapeutic tools that can be produced by phage display technology (PDT).
Shirafkan Kordi +4 more
doaj +1 more source
Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells [PDF]
Advanced Pharmaceutical Bulletin, 2017 Purpose: More than half of the diagnostic and therapeutic recombinant protein production depends on mammalian-based expression system. However, the generation of recombinant antibodies remains a challenge in mammalian cells due to the disulfide bond ...
Mohammad Tasyriq Che Omar
doaj +1 more source
A strategy for adenovirus vector targeting with a secreted single chain antibody. [PDF]
PLoS ONE, 2009 BACKGROUND:Successful gene therapy will require targeted delivery vectors capable of self-directed localization. In this regard, the use of antibodies or single chain antibody fragments (scFv) in conjunction with adenovirus (Ad) vectors remains an ...
Joel N Glasgow +3 more
doaj +1 more source
Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation
FEBS Letters, EarlyView.Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang +2 more
wiley +1 more source
Research in Pharmaceutical SciencesBackground and purpose: The estrogen receptor alpha-36 (ER-α36) is an alternative splice variant of classical ER-α66 and is abundantly present in both ER-α66-positive and ER-α66-negative breast tumor cells.
Soodabeh Shafiee +7 more
doaj +1 more source
Redirecting Monocyte Differentiation With Engineered Extracellular Vesicles for Glioma Immunotherapy
Advanced Science, EarlyView.A dual‐targeting engineered extracellular vesicles (M1‐CS‐EVs) platform is developed to redirect monocytes differentiation into anti‐tumor macrophages for glioma immunotherapy. This nanoplatform combines CAR‐mediated tumor recognition with localized CD47 blockade, leading to synergistic immune activation and potent suppression of tumor progression in ...
Yuanwei Pan +9 more
wiley +1 more source