Results 21 to 30 of about 35,905 (251)

Folding Nuclei of the scFv Fragment of an Antibody [PDF]

Biochemistry, 1996
The folding kinetics of the variable domains of the phosphorylcholine-binding antibody McPC603, combined into a scFv fragment [VH-(Gly4Ser)3-VL], were investigated by the use of fluorescence spectroscopy, nuclear magnetic resonance (NMR), and mass spectrometry (MS).
Freund C, Honegger A, Hunziker P, Holak TA, Plückthun A   +4 more
openaire   +3 more sources

Toward low-cost affinity reagents: lyophilized yeast-scFv probes specific for pathogen antigens. [PDF]

PLoS ONE, 2012
The generation of affinity reagents, usually monoclonal antibodies, remains a critical bottleneck in biomedical research and diagnostic test development.
Sean A Gray, Kris M Weigel, Ibne K M Ali, Annie A Lakey, Jeremy Capalungan, Gonzalo J Domingo, Gerard A Cangelosi   +6 more
doaj   +1 more source

Selection, alkaline phosphatase fusion, and application of single-chain variable fragment (scFv) specific to NT-proBNP as electrochemical immunosensor for heart failure

Heliyon, 2023
Heart failure has a high global prevalence, with symptoms such as breathlessness, fatigue, and swelling. Early detection is crucial, as the condition worsens over time and can be fatal. This study identified the single-chain variable fragment (scFv) that
Sureeporn Wongjard, Pongsakorn Aiemderm, Kanchana Monkhang, Kittitat Jaengwang, Lueacha Tabtimmai, Charoenkwan Kraiya, Kiattawee Choowongkomon, Napachanok Mongkoldhumrongkul Swainson   +7 more
doaj   +1 more source

Chicken scFvs and bivalent scFv-CH fusions directed against HSP65 of Mycobacterium bovis [PDF]

Biologicals, 2010
Two chicken single-chain variable region antibody fragments (scFvs) that recognised the 65 kDa heat-shock protein (HSP65) of Mycobacterium bovis were selected from a large semi-synthetic phage displayed library. Both recognised HSP65 in indirect enzyme-linked immunosorbent assay (ELISA) and immunoblots and retained their activity during storage ...
Wemmer, Susan, Mashau, Cordelia, Fehrsen, Jeanni, Van Wyngaardt, Wouter, Du Plessis, Dion H.   +4 more
openaire   +3 more sources

Combinations of Single Chain Variable Fragments From HIV Broadly Neutralizing Antibodies Demonstrate High Potency and Breadth

Frontiers in Immunology, 2021
Broadly neutralizing antibodies (bNAbs) are currently being assessed in clinical trials for their ability to prevent HIV infection. Single chain variable fragments (scFv) of bNAbs have advantages over full antibodies as their smaller size permits ...
Rebecca T. van Dorsten, Rebecca T. van Dorsten, Kshitij Wagh, Penny L. Moore, Penny L. Moore, Penny L. Moore, Lynn Morris, Lynn Morris, Lynn Morris   +8 more
doaj   +1 more source

Nonspecificity in a nonimmune human scFv repertoire [PDF]

mAbs, 2017
Efforts to develop effective antibody therapeutics are frequently hampered by issues such as aggregation and nonspecificity, often only detected in late stages of the development process. In this study, we used a high throughput cross-reactivity assay to select nonspecific clones from a naïve human repertoire scFv library displayed on the surface of ...
Kelly, Ryan L., Zhao, Jessie, Le, Doris, Wittrup, K. Dane   +3 more
openaire   +2 more sources

A Cassette Vector System for the Rapid Cloning and Production of Bispecific Tetravalent Antibodies

Antibodies, 2012
Bivalent single chain (sc)Fv-Fc antibodies have been used for years as recombinant alternatives of natural immunoglobulins. We have extended this approach to the scFv-Fc-scFv antibody format to obtain tetravalent antigen binding and the possibility to ...
Stefanie Claudia Pohl, Steffi Schwarz, André Frenzel, Thomas Schirrmann   +3 more
doaj   +1 more source

INCREASING OF THE EXPRESSION OF RECOMBINANT scFv-ANTIBODIES EFFICIENCY [PDF]

Biotechnologia Acta, 2017
Obtaining single-chain variable fragments (scFv) of recombinant antibodies in E. coli cells is often associated with numerous problems causing low yields or inactive conformation of the product.
O.V. Galkin, T.O. Chudina, A.A. Siromolot, O.S. Oliynyk   +3 more
doaj   +1 more source

Engineering Peptide Linkers for scFv Immunosensors [PDF]

Analytical Chemistry, 2008
Using A10B single-chain fragment variable (scFv) as a model system, we demonstrated that the flexibility of scFv linker engineering can be combined with the inherent quick and adaptable characters of surface coupling chemistry (e.g., electrostatic, hydrogen bonding, or covalent attachment) to attach scFv to preformed functionalized self-assembled ...
Zhihong, Shen, Heping, Yan, Ying, Zhang, Raymond L, Mernaugh, Xiangqun, Zeng   +4 more
openaire   +2 more sources

Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update

Molecular Biotechnology, 2020
Single chain variable fragments (scFvs) are generated by joining together the variable heavy and light chain of a monoclonal antibody (mAb) via a peptide linker.
P. Satheeshkumar
semanticscholar   +1 more source