PLoS ONE, 2011 The ubiquitin-like protein NEDD8 is highly conserved in eukaryotes, from man to Schizosaccharomyces pombe. NEDD8 conjugation to cullin proteins is a prerequisite for cullin based E3 ubiquitin ligase activity, and essential for S. pombe viability.
David Girdwood +2 more
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The Rad4TopBP1 ATR-Activation domain functions in G1/S phase in a chromatin-dependent manner [PDF]
, 2012 DNA damage checkpoint activation can be subdivided in two steps: initial activation and signal amplification. The events distinguishing these two phases and their genetic determinants remain obscure.
Carr, Antony +8 more
core +10 more sources
Journal of Fungi, 2021 We have characterized a homodimeric tRNA endonuclease from the euryarchaeota Ferroplasma acidarmanus (FERAC), a facultative anaerobe which can grow at temperatures ranging from 35 to 42 °C. This enzyme, contrary to the eukaryal tRNA endonucleases and the
Giuseppe D. Tocchini-Valentini +1 more
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Curvature-induced expulsion of actomyosin bundles during cytokinetic ring contraction
eLife, 2016 Many eukaryotes assemble a ring-shaped actomyosin network that contracts to drive cytokinesis. Unlike actomyosin in sarcomeres, which cycles through contraction and relaxation, the cytokinetic ring disassembles during contraction through an unknown ...
Junqi Huang +9 more
doaj +1 more source
Electron tomography reveals novel microtubule lattice and microtubule organizing centre defects in +TIP mutants. [PDF]
PLoS ONE, 2013 Mal3p and Tip1p are the fission yeast (Schizosaccharomyces pombe) homologues of EB1 and CLIP-170, two conserved microtubule plus end tracking proteins (+TIPs). These proteins are crucial regulators of microtubule dynamics.
Johanna L Höög +3 more
doaj +1 more source
Spd2 assists Spd1 in modulation of RNR architecture but does not regulate deoxynucleotide pools [PDF]
, 2014 In yeasts, small intrinsically disordered proteins (IDPs) modulate ribonucleotide reductase (RNR) activity to ensure an optimal supply of dNTPs for DNA synthesis. The S. pombe Spd1 protein can directly inhibit the large RNR subunit (R1), import the small
Basrai +37 more
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Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA [PDF]
, 2015 SMC5/6 is a highly conserved protein complex related to cohesin and condensin, which are the key components of higher-order chromatin structures. The SMC5/6 complex is essential for proliferation in yeast and is involved in replication fork stability and
Adamus, Marek +10 more
core +2 more sources
The <i>Schizosaccharomyces pombe</i> nucleolar protein Nsk1 modulates rDNA silencing during interphase. [PDF]
MicroPubl BiolSchizosaccharomyces pombe Nsk1 acts at kinetochores during mitosis to prevent error-prone chromosome segregation and it is phosphoregulated by Cdk1 . The Clp1 / Cdc14 protein phosphatase, to which Nsk1 binds, reverses Cdk1-mediated phosphorylation of Nsk1 during anaphase.
Chen JS, Hanna SM, Willet AH, Gould KL.
europepmc +3 more sources
Distinct requirements for the Rad32(Mre¹¹) nuclease and Ctp1(CtIP) in the removal of covalently bound topoisomerase I and II from DNA [PDF]
, 2009 For a cancer cell to resist treatment with drugs that trap topoisomerases covalently on the DNA, the topoisomerase must be removed. In this study, we provide evidence that the Schizosaccharomyces pombe Rad32Mre11 nuclease activity is involved in the ...
Akamatsu +38 more
core +2 more sources
Boolean network model predicts cell cycle sequence of fission yeast. [PDF]
PLoS ONE, 2008 A Boolean network model of the cell-cycle regulatory network of fission yeast (Schizosaccharomyces Pombe) is constructed solely on the basis of the known biochemical interaction topology.
Maria I Davidich, Stefan Bornholdt
doaj +1 more source