Results 1 to 10 of about 22,058 (247)

Advances in Aptamer-Based Biosensors and Cell-Internalizing SELEX Technology for Diagnostic and Therapeutic Application [PDF]

Biosensors, 2022
Aptamers are a group of synthetic single-stranded nucleic acids. They are generated from a random library of single-stranded DNA or RNA by a technology named systematic evolution of ligands by exponential enrichment (SELEX). SELEX is a repetitive process
Zixuen Gan, Muhamad Aidilfitri Mohamad Roslan, Mohd Yunus Abd Shukor, Murni Halim, Nur Adeela Yasid, Jaafar Abdullah, Ina Salwany Md Yasin, Helmi Wasoh   +7 more
doaj   +2 more sources

Inside the Black Box: What Makes SELEX Better?

Molecules, 2019
Aptamers are small oligonucleotides that are capable of binding specifically to a target, with impressive potential for analysis, diagnostics, and therapeutics applications.
Natalia Komarova, Alexander E Kuznetsov
exaly   +3 more sources

Recent Advances in the Optimization of Nucleic Acid Aptamers and Aptasensors [PDF]

Biosensors
Nucleic acid aptamers are single-stranded DNA or RNA molecules that can bind to a target with high specificity and affinity, as screened by the Systematic Evolution of Ligands by Exponential Enrichment (SELEX).
Yuan Wang, Mengyan Nie
doaj   +2 more sources

Advantages, applications, and future directions of in vivo aptamer SELEX: A review [PDF]

Molecular Therapy: Nucleic Acids
In vivo systematic evolution of ligands by exponential enrichment (SELEX) emerged as a transformative technique for directly identifying aptamers within living organisms.
Minchuan Lyu, Chi Ho Chan, Ziqi Chen, Yumeng Liu, Yuanyuan Yu   +4 more
doaj   +2 more sources

Development of RNA G-quadruplex (rG4)-targeting l-RNA aptamers by rG4-SELEX [PDF]

Nature Protocols, 2022
Mubarak I. Umar, Chun-Yin Chan, Chun Kit Kwok   +2 more
exaly   +2 more sources

Green Fluorescent Protein SELEX: Immobilization Chemistry and His-Tag Epitope Bias. [PDF]

Angew Chem Int Ed Engl
Stangherlin S, Malloch T, Clarke AJ, Liu J.   +3 more
europepmc   +3 more sources

The Potential Use of Aptamers in The Process of Drug Development [PDF]

Pharmaceutical Sciences, 2021
Single-stranded nucleic acids can fold and create unique 3-dimensional structures when interacting with other molecules. The unique structure can achieve high specificity and affinity for the particular target.
Tooba Gholikhani, Balam Jimenez Brito, Frey Livingston, Shalen Kumar   +3 more
doaj   +1 more source

Development of DNA aptamer selection approach based on membrane ultrafiltration of aptamer/target complex

Acta Biomedica Scientifica, 2022
Background. Aptamers are small single-stranded DNA or RNA molecules that have an affinity for a specific target molecule. The main method of aptamers construction is the technology of systematic evolution of ligands with exponential enrichment (SELEX ...
I. S. Solovarov, M. A. Khasnatinov, N. A. Liapunova, I. G. Kondratov, G. A. Danchinova   +4 more
doaj   +1 more source

Engineering Novel Aptameric Fluorescent Biosensors for Analysis of the Neurotoxic Environmental Contaminant Insecticide Diazinon from Real Vegetable and Fruit Samples

Frontiers in Bioscience-Landmark, 2022
Background: Diazinon is a widely used organophosphorus neurotoxic insecticide. It is a common environmental contaminant and a hazardous agri-waste. Its detection is critical to control entry into food systems and protect the environment. Methods: In this
Mai-Huong Thi Can, Ulhas Sopanrao Kadam, Kien Hong Trinh, Yuhan Cho, Hyebi Lee, Yujeong Kim, Sundong Kim, Chang Ho Kang, Sang Hee Kim, Woo Sik Chung, Sang Yeol Lee, Jong Chan Hong   +11 more
doaj   +1 more source

Complementary chain competition and fluorescence quenching detection of Deoxynivalenol and analytical applications using a novel aptamer

CyTA - Journal of Food, 2021
Deoxynivalenol (DON) is a mycotoxin commonly found in cereals and animal feeds, which also is a carcinogen easily soluble in water. So it has drawn considerable interest in food safety.
Mingwei Qin, Xiaomeng Zhang, Xinyue Zhao, Yuzhu Song, Jinyang Zhang, Xueshan Xia, Qinqin Han   +6 more
doaj   +1 more source