Results 171 to 180 of about 14,594 (219)

Blocker-SELEX: a structure-guided strategy for developing inhibitory aptamers disrupting undruggable transcription factor interactions

bioRxiv
Despite the well-established significance of transcription factors (TFs) in pathogenesis, their utilization as pharmacological targets has been limited by the inherent challenges associated with modulating their protein-protein and protein-DNA ...
Tongqing Li, Xueying Liu, Haifeng Qian, Sheyu Zhang, Yu Hou, Yuchao Zhang, Guoyan Luo, Xun Zhu, Yanxin Tao, Mengyang Fan, Hong Wang, Chulin Sha, A. Lin, Jingjing Qin, Kedan Gu, Weichang Chen, Ting Fu, Yajun Wang, Yong Wei, Qin Wu, Weihong Tan   +20 more
semanticscholar   +1 more source

In vitro selection and characterization of ssDNA aptamers by cross-over SELEX and its application for detection of S. Typhimurium.

Analytical Biochemistry, 2022
S. Typhimurium is the most common food-borne pathogen frequently encountered in contaminated food and poses a major threat to public health, animals, and the food industry worldwide.
P. Nikam, S. Palachandra, J. Kingston
semanticscholar   +1 more source

SelEx: Self-Expertise in Fine-Grained Generalized Category Discovery

European Conference on Computer Vision
In this paper, we address Generalized Category Discovery, aiming to simultaneously uncover novel categories and accurately classify known ones. Traditional methods, which lean heavily on self-supervision and contrastive learning, often fall short when ...
Sarah Rastegar, Mohammadreza Salehi, Yuki M. Asano, Hazel Doughty, Cees G. M. Snoek   +4 more
semanticscholar   +1 more source

Process, advances, and perspectives of graphene oxide-SELEX for the development of aptamer molecular probes: A comprehensive review.

Analytica Chimica Acta
BACKGROUND Aptamers are screened via the systematic evolution of ligands by exponential enrichment (SELEX) and are widely used in molecular diagnostics and targeted therapies.
Zhuoer Chen, Ying Yang, Xinge Cui, Luwei Chai, Hongbing Liu, Yangwei Pan, Yongkang Zhang, Yujia Xie, Tao Le   +8 more
semanticscholar   +1 more source

SELEX: Just another separation?

The Analyst, 2005
The potential for quickly isolating high affinity, highly selective ligands generated much excitement when SELEX was first described. Fifteen years later, SELEX has still not achieved widespread acceptance due to limitations in aptamer affinity, stability and throughput.
openaire   +2 more sources

Aptamers: The “evolution” of SELEX

Methods, 2016
It has been more than two decades since the first aptamer molecule was discovered. Since then, aptamer molecules have gain much attention in the scientific field. This increasing traction can be attributed to their many desirable traits, such as 1) their potentials to bind a wide range of molecules, 2) their malleability, and 3) their low cost of ...
Yi Xi, Wu, Young Jik, Kwon
openaire   +2 more sources

Combination of Capture-SELEX and Post-SELEX for procymidone-specific aptamer selection and broad-specificity aptamer discovery, and development of aptamer-based lateral flow assay.

Analytica Chimica Acta
BACKGROUND Due to its wide application, procymidone has become one of the pesticides with high detection rates in supervision and sampling. Therefore, it is necessary to establish a rapid and efficient method for the detection of procymidone. However, an
H. Dong, Haifang Wang, Zhen Guo, Jingcheng Huang, Pengwei Zhang, Yemin Guo, Xia Sun   +6 more
semanticscholar   +1 more source

RNA–RNA SELEX

2014
Systematic evolution of ligands by exponential enrichment (SELEX) protocol is a valuable technique to identify RNA aptamers interacting with RNA structural motifs. RNA aptamers are mainly resolved with affinity column chromatography and electrophoretic mobility shift assay (EMSA).
openaire   +2 more sources

RECENT FOCUS IN NON-SELEX-COMPUTATIONAL APPROACH FOR DE NOVO APTAMER DESIGN: A MINI REVIEW.

Analytical Biochemistry
Aptamers, single-stranded nucleic acids that bind to specific targets with high affinity and specificity, hold significant promise in various biomedical and biotechnological applications.
I. Fasogbon, E. N. Ondari, D. Tusubira, Loganathan Rangasamy, Janarthanan Venkatesan, Angela Mumbua Musyoka, P. Aja   +6 more
semanticscholar   +1 more source

Methods developed for SELEX

Analytical and Bioanalytical Chemistry, 2006
SELEX (systematic evolution of ligands by exponential enrichment) is a process that involves the progressive purification from a combinatorial library of nucleic acid ligands with a high affinity for a particular target by repeated rounds of partitioning and amplification.
openaire   +2 more sources