Results 51 to 60 of about 22,058 (247)

High throughput sequencing analysis of RNA libraries reveals the influences of initial library and PCR methods on SELEX efficiency. [PDF]

, 2016
The systemic evolution of ligands by exponential enrichment (SELEX) technique is a powerful and effective aptamer-selection procedure. However, modifications to the process can dramatically improve selection efficiency and aptamer performance.
Burnett, John C, Chomchan, Pritsana, Ho, Michelle, Rossi, John J, Takahashi, Mayumi, Wu, Xiwei, Zhou, Jiehua   +6 more
core  

First observation of a narrow charm-strange meson DsJ(2632) -> Ds eta and D0 K+

, 2004
We report the first observation of a charm-strange meson DsJ(2632) at a mass of 2632.6+/-1.6 MeV/c^2 in data from SELEX, the charm hadro-production experiment E781 at Fermilab. This state is seen in two decay modes, Ds eta and D0 K+.
A. A., Vorobyov, A. G., Atamantchouk, A. G., Dolgolenko, A. G., Krivshich, A. I., Kulyavtsev, A. I., Sitnikov, A. N., Vasiliev, A. P., Kozhevnikov, A. P., Nilov, A. S., Ayan, A. V., Evdokimov, A. V., Nemitkin, A., Kushnirenko, A., Lamberto, A., Morelos, A., Ocherashvili, A., Penzo, Amf, Endler, B. V., Razmyslovich, C. O., Escobar, C., Newsom, D. V., Vavilov, D., Mao, D., Skow, E. M., Leikin, E., Gulmez, E., Mccliment, E., Ozel, E., Ramberg, E., Vazquez Jauregui, F. G., Garcia, G. B., Dzyubenko, G. F., Rappazzo, G. V., Davidenko, G., Alkhazov, Gaspero, Mario, H., Kangling, H., Kruger, I. S., Filimonov, I., Eschrich, I., Giller, I., Konorov, I., Larin, I., Torres, J., Amaro Reyes, J., Engelfried, J., Kilmer, J., Lach, J., Russ, J., Simon, J., You, K. D., Nelson, K., Vorwalter, L. G., Landsberg, L. J., Dauwe, L. M., Kochenda, L. N., Uvarov, L., Emediato, L., Stutte, L., Yunshan, M. A., Kubantsev, M. A., Moinester, M. Y., Balatz, M., Chensheng, M., Iori, M., Kaya, M., Luksys, M., Mattson, M., Procario, M., Srivastava, M., Svoiski, M., Zhenlin, N. F., Bondar, N. K., Terentyev, N. P., Kuropatkin, P. S., Cooper, P. V., Neoustroev, P., Gouffon, P., Mathew, P., Pogodin, P., Schiavon, R., Edelstein, R., Zukanovich Funchal, S. B., Nurushev, S. V., Petrenko, S. Y., Jun, S., Kwan, S., Ozkorucuklu, T., Lungov, Thomas, Gp, U., Akgun, U., Dersch, V. A., Victorov, V. E., Vishnyakov, V. F., Kurshetsov, V. I., Rud, V. J., Smith, V. L., Golovtsov, V. P., Kubarovsky, V. P., Maleev, V. S., Verebryusov, V. T., Kim, V. V., Molchanov, V., Matveev, V., Steiner, V., Stepanov, Y., Onel, Z., Shuchen, Z., Wenheng   +108 more
core   +1 more source

DNA Origami‐Templated Aptamer Chiral Structures Realize Cellular Enantioselectivity

Advanced Materials, EarlyView.
Aptamers displayed on tubular DNA origami nanostructures in defined chiral orientations (left‐ or right‐handed) exhibit enantioselective interactions with cell surface target proteins. While the right‐handed configuration supports only transient binding, the left handedness energetically favors protein dimerization and promotes subsequent ...
Tingjie Song, Abhisek Dwivedy, Dhanush Gandavadi, Gayatri Chandran, Yiquan An, Mengxi Zheng, Xiaojing Wang, Lifeng Zhou, Yang Zhao, Xing Wang   +9 more
wiley   +1 more source

A mathematical analysis of SELEX [PDF]

Computational Biology and Chemistry, 2007
Systematic evolution of ligands by exponential enrichment (SELEX) is a procedure by which a mixture of nucleic acids that vary in sequence can be separated into pure components with the goal of isolating those with specific biochemical activities. The basic idea is to combine the mixture with a specific target molecule and then separate the target-NA ...
Howard A, Levine, Marit, Nilsen-Hamilton
openaire   +2 more sources

Mammalian Proteome Profiling Reveals Readers and Antireaders of Strand‐Symmetric and ‐Asymmetric 5‐Hydroxymethylcytosine‐Modifications in DNA

Advanced Science, EarlyView.
We investigate by proteomics studies how strand‐symmetric and ‐asymmetric cytosine 5‐modifications in DNA are selectively recognized by the nuclear proteome. Using promoter probes with defined modification patterns, we identify tissue‐specific reader proteinsincluding MYC, MAX, and RFX5that discriminate 5‐hydroxymethylcytosine symmetry and sequence ...
Lena Engelhard, Zeyneb Vildan Cakil, Marlon S. Zambrano‐Mila, Simone Eppmann, Tye Gonzalez, Rasmus Linser, Petra Janning, Sidney Becker, Daniel Summerer   +8 more
wiley   +1 more source

A novel hybrid approach to overcome defects of CE-SELEX and cell-SELEX in developing aptamers against aspartate β-hydroxylase

Research in Pharmaceutical Sciences
Background and purpose: Aptamers, a new category of molecular probes, are overthrowing antibodies in molecular diagnostics. However, there are serious problems with using aptamers for this application including poor or non-specific binding in vivo ...
Hadi Bakhtiari, Hamed Naghoosi, Sina Sattari, Mahmoud Vahidi, Mehdi Shakouri Khomartash, Ali Faridfar, Mohsen Rajaeinejad, Mohsen Nikandish   +7 more
doaj   +1 more source

SELEX: Recent Progress in the Analysis of Charm-Strange and Double-Charm Baryons [PDF]

, 2007
We present recent progress in the analysis of the Omega_c^0, the Xi_cc^+ and the Xi_cc^++.Comment: Contribution to the proceedings of HQL06, Munich, October 16th-20th ...
Engelfried, Jurgen
core   +1 more source

DNA Nanostructure‐Templated Multivalency Enables Broad‐Spectrum Virus Inhibition

Advanced Science, EarlyView.
A honeycomb‐shaped DNA nanostructure is introduced that organizes nanobodies or aptamers into trimeric clusters mirroring the native hemagglutinin arrangement on influenza viruses. This geometry‐matched multivalency enhances binding avidity by up to 1000‐fold, resulting in robust, broad‐spectrum viral neutralization and a 30–55% improvement in host ...
Saurabh Umrao, Abhisek Dwivedy, Dhanush Gandavadi, Chi Chen, Lifeng Zhou, Jinwei Duan, Vineetha Mareddy, Ying Fang, Xing Wang   +8 more
wiley   +1 more source

Aptamer Cell-Based Selection: Overview and Advances

Biomedicines, 2017
Aptamers are high affinity single-stranded DNA/RNA molecules, produced by a combinatorial procedure named SELEX (Systematic Evolution of Ligands by Exponential enrichment), that are emerging as promising diagnostic and therapeutic tools.
Silvia Catuogno, Carla Lucia Esposito
doaj   +1 more source

Evolution of Multivalent Aptamer Corona for High‐Throughput Multiplexed Detection of Multiple Cancers

Advanced Science, EarlyView.
A multivalent aptamer corona is evolved via established ProteoFish‐SELEX (systematic evolution of ligands by exponential enrichment) for high‐throughput multiplexed detection of multiple cancers. Clinical validation using multivalent aptamer corona demonstrated high accuracy and specificity in multiplexed detection of ovarian, lung, and colorectal ...
Mengjie Wang, Baichuan Jin, Xin Dai, Haozhe Huang, Qiqi Liu, Jianqing Zhu, Haixing Ju, Qixun Chen, Yongmei Song, Weihong Tan, Yuan Liu   +10 more
wiley   +1 more source