Results 51 to 60 of about 56,769 (258)

Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine candidate induces high neutralizing antibody titers in mice

Nature Communications, 2020
Here, the authors develop a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle as a vaccine candidate and show induction of neutralization antibody titers in mice that are comparable to titers in ...
Paul F. McKay, Kai Hu, Anna K. Blakney, Karnyart Samnuan, Jonathan C. Brown, Rebecca Penn, Jie Zhou, Clément R. Bouton, Paul Rogers, Krunal Polra, Paulo J. C. Lin, Christopher Barbosa, Ying K. Tam, Wendy S. Barclay, Robin J. Shattock   +14 more
doaj   +1 more source

Rescue of splicing-mediated intron loss maximizes expression in lentiviral vectors containing the human ubiquitin C promoter. [PDF]

, 2014
Lentiviral vectors almost universally use heterologous internal promoters to express transgenes. One of the most commonly used promoter fragments is a 1.2-kb sequence from the human ubiquitin C (UBC) gene, encompassing the promoter, some enhancers, first
Cooper, Aaron R, Gschweng, Eric H, Kohn, Donald B, Lill, Georgia R   +3 more
core   +1 more source

A new generation of vaccines based on alphavirus self-amplifying RNA

Current Opinion in Virology, 2020
DNA or mRNA vaccines have potential advantages over conventional vaccines since they are easier to manufacture and have higher safety profiles. In particular, self-amplifying RNA (saRNA) derived from alphavirus expression vectors has shown to be very efficient to induce humoral and cellular responses against many antigens in preclinical models, being ...
Ballesteros-Briones, María Cristina, Silva-Pilipich, Noelia, Herrador-Cañete, Guillermo, Vanrell, Lucia, Smerdou, Cristian   +4 more
openaire   +2 more sources

Trans-Amplifying RNA: A Journey from Alphavirus Research to Future Vaccines

Viruses
Replicating RNA, including self-amplifying RNA (saRNA) and trans-amplifying RNA (taRNA), holds great potential for advancing the next generation of RNA-based vaccines.
Ayşegül Yıldız, Cristian Răileanu, Tim Beissert   +2 more
doaj   +1 more source

Controlling plant architecture by manipulation of gibberellic acid signalling in petunia. [PDF]

, 2014
Since stem elongation is a gibberellic acid (GA) response, GA inhibitors are commonly used to control plant height in the production of potted ornamentals and bedding plants. In this study, we investigated interfering with GA signaling by using molecular
Jiang, Cai-Zhong, Liang, Yin-Chih, Reid, Michael S   +2 more
core   +3 more sources

CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding [PDF]

, 2020
Open Access Journal; Published online: 31 Jan 2019Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids.
Britt, A., Muiruri, S.K., Ntui, V.O., Ron, M., Tripathi, J.N., Tripathi, L.   +5 more
core   +1 more source

Vaccines’ New Era-RNA Vaccine

Viruses, 2023
RNA vaccines, including conventional messenger RNA (mRNA) vaccines, circular RNA (circRNA) vaccines, and self-amplifying RNA (saRNA) vaccines, have ushered in a promising future and revolutionized vaccine development.
Wenshuo Zhou, Linglei Jiang, Shimiao Liao, Feifei Wu, Guohuan Yang, Li Hou, Lan Liu, Xinping Pan, William Jia, Yuntao Zhang   +9 more
doaj   +1 more source

DGR mutagenic transposition occurs via hypermutagenic reverse transcription primed by nicked template RNA. [PDF]

, 2017
Diversity-generating retroelements (DGRs) are molecular evolution machines that facilitate microbial adaptation to environmental changes. Hypervariation occurs via a mutagenic retrotransposition process from a template repeat (TR) to a variable repeat ...
Guo, Huatao, Han, Jin, Heng, Xiao, Lee, William R, Miller, Jeff F, Naorem, Santa S, Wang, Shufang   +6 more
core   +3 more sources

A transient homotypic interaction model for the influenza A virus NS1 protein effector domain [PDF]

, 2011
Influenza A virus NS1 protein is a multifunctional virulence factor consisting of an RNA binding domain (RBD), a short linker, an effector domain (ED), and a C-terminal 'tail'. Although poorly understood, NS1 multimerization may autoregulate its actions.
A Cheng, A Hayman, A Senes, Adolfo García-Sastre, AJ McCoy, Andrew Lewis, B Keiner, Benjamin G. Hale, BG Hale, BG Hale, BG Hale, BG Hale, BG Hale, BG Hale, C Reynolds, C Yin, C Zhao, CCP4, Claudia Hass, CY Chien, G Kochs, H Liu, H Niwa, I Burgui, IM Nooren, J Janin, J Liu, JR Perkins, Juan Ayllon, JX Long, JY Min, K Das, K Xu, KY Twu, LS Heikkinen, M Yoneyama, Margaret A. Taylor, MD Abramoff, ME Nemeroff, ME Nemeroff, MU Gack, NR Donelan, P Emsley, Paul Digard, PD Adams, Philip S. Kerry, Q Xu, R Volmer, Richard E. Randall, RP Bahadur, RP Saha, Rupert J. Russell, S Dey, S Li, S Steidle, S Xia, S Xia, Schrodinger, SH Seo, TA Jones, W Wang, WP Russ, X Wang, XY Qian, Z Otwinowski, ZA Bornholdt, ZA Bornholdt, ZJ Miknis   +67 more
core   +9 more sources

Universal Flu mRNA Vaccine: Promises, Prospects, and Problems

Vaccines, 2022
The seasonal flu vaccine is, essentially, the only known way to prevent influenza epidemics. However, this approach has limited efficacy due to the high diversity of influenza viruses. Several techniques could potentially overcome this obstacle. A recent
Andrei A. Deviatkin, Ruslan A. Simonov, Kseniya A. Trutneva, Anna A. Maznina, Elena M. Khavina, Pavel Y. Volchkov   +5 more
doaj   +1 more source