Results 41 to 50 of about 5,246,093 (238)

Enhanced Delivery and Potency of Self-Amplifying mRNA Vaccines by Electroporation in Situ

Vaccines, 2013
Nucleic acid-based vaccines such as viral vectors, plasmid DNA (pDNA), and mRNA are being developed as a means to address limitations of both live-attenuated and subunit vaccines.
Kaustuv Banerjee, Julie Hickman, Andrew Geall, Jeffrey B. Ulmer, Gleb Kichaev, Niranjan Y. Sardesai, Yen Cu, Susan Barnett, Gillis Otten, Kate E. Broderick   +9 more
doaj   +1 more source

Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine candidate induces high neutralizing antibody titers in mice

Nature Communications, 2020
Here, the authors develop a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle as a vaccine candidate and show induction of neutralization antibody titers in mice that are comparable to titers in ...
Paul F. McKay, Kai Hu, Anna K. Blakney, Karnyart Samnuan, Jonathan C. Brown, Rebecca Penn, Jie Zhou, Clément R. Bouton, Paul Rogers, Krunal Polra, Paulo J. C. Lin, Christopher Barbosa, Ying K. Tam, Wendy S. Barclay, Robin J. Shattock   +14 more
doaj   +1 more source

Non-viral delivery of self-amplifying RNA

, 2012
Despite two decades of research on nucleic acid vaccines, there is still no commercial product for human use. Taking advantage of the recent innovations around systemic delivery of siRNA using lipid nanoparticles (LNPs), we developed a self-amplifying ...
Valiante, Nicholas, Banerjee, Kaustuv, Verma, Ayush, Barnett, Susan, Rappuoli, Rino, Ulmer, Jeffrey, Mandl, Christian, Brito, Luis, Shaw, Christine, Hekele, Armin, Krucker, Thomas, Singh, Manmohan, Beard, Clayton, Dormitzer, Philip, Otten, Gillis, Geall, Andrew, Cu, Yen, O'Hagan, Derek, Mason, Peter   +18 more
core   +1 more source

Domain orientation in the RNA helicase YxiN and the role of conformational changes for RNA unwinding [PDF]

, 2010
The RNA helicase YxiN from Bacillus subtilis is a member of the family of DEAD box proteins. YxiN is able to unwind RNA double strands in an ATP-dependent manner. The ability to catalyse RNA rearrangement is in vivo presumably necessary for the bacterial
Karow, Anne R.
core   +1 more source

Engineering a regulatory framework for synthetic self-amplifying RNA circuits [PDF]

, 2017
Self-amplifying RNA replicons are an attractive alternative to traditional nucleic acid therapeutics, providing high, sustained expression from a low dose, without the risk of genomic integration.
Wagner, Tyler
core   +1 more source

Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons [PDF]

, 2020
Axonal protein synthesis has been shown to play a role in developmental and regenerative growth, as well as in the maintenance of the axoplasm in steady state.
Sotelo-Silveira, José Roberto, Farías, Joaquina, Sotelo Sosa, José Roberto, Holt, C. E.   +3 more
core   +3 more sources

Polyethylenimine-based polyplex delivery of self-replicating RNA vaccines [PDF]

, 2015
Self-amplifying replicon RNA (RepRNA) are large molecules (12-14kb); their self-replication amplifies mRNA template numbers, affording several rounds of antigen production, effectively increasing vaccine antigen payloads.
Ebensen, Thomas, McCullough, Kenneth, Demoulins, Thomas, Mccullough, Kenneth C., Englezou, Pavlos C., Ruggli, Nicolas, Guzmán, Carlos A., Englezou, Pavlos, Midoux, Patrick, Milona, Panagiota, Schulze, Kai, Pichon, Chantal, Guzmán, Carlos A, Suter, Rolf   +13 more
core   +1 more source

Complete probabilistic analysis of RNA shapes [PDF]

, 2006
Voß B, Giegerich R, Rehmsmeier M. Complete probabilistic analysis of RNA shapes. BMC Biology. 2006;4(1): 5.Background: Soon after the first algorithms for RNA folding became available, it was recognised that the prediction of only one energetically ...
Marc Rehmsmeier, Voß, Björn, Rehmsmeier, Marc, Björn Voß, Rehmsmeier Marc, Robert Giegerich, Giegerich, Robert, Giegerich Robert, Voß Björn   +8 more
core   +1 more source

Epigenetic reprogramming of lineage switching in cancer

FEBS Letters, EarlyView.
Cancer cells rarely commit to a single identity. Epigenetic mechanisms and tumor microenvironment cues push epithelial cells toward flexible, hybrid states that can shift into mesenchymal, neuroendocrine, or stem‐like fates, driving metastasis, drug resistance, and tumor heterogeneity. Targeting the epigenetic regulators behind these transitions, using
Ezgi Boyvatlı, Burcu Akman, Ezgi Bağırsakçı, Nese Atabey, Serap Erkek‐Ozhan   +4 more
wiley   +1 more source

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

FEBS Letters, EarlyView.
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley   +1 more source