Results 51 to 60 of about 5,246,093 (238)

Internship Reports and Monograph Entitled"New RNA Therapeutic Approaches: Circular RNA, Self-amplifying RNA and Trans-amplifying RNA"

, 2023
Relatório de Estágio do Mestrado Integrado em Ciências Farmacêuticas apresentado à Faculdade de FarmáciaThis document includes the Internship Reports in Community Pharmacy and Pharmaceutical Industry and the Monograph entitled “New Therapeutic Approaches
Neves, Beatriz Fernandes das
core  

CEACAM1 participation in breast cancer progression

Molecular Oncology, EarlyView.
In invasive breast cancer (BC), CEACAM1 shifts from an apical to a uniform membranous/cytoplasmic pattern, or is lost, as tumors dedifferentiate, inversely tracking the Ki‐67 proliferative index. In MCF‐7 cells, only CEACAM1‐4L suppresses proliferation, repressing cell cycle and growth factor genes.
Mykola Lyndin, Irina Kube‐Golovin, Anatolii Romaniuk, Gunther Wennemuth   +3 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

A hepatitis C virus cis-acting replication element forms a long-range RNA-RNA interaction with upstream RNA sequences in NS5B [PDF]

, 2008
The genome of hepatitis C virus (HCV) contains cis-acting replication elements (CREs) comprised of RNA stem-loop structures located in both the 5' and 3' non-coding regions (NCR), and in the NS5B coding sequence.
Diviney, Sinéad, Elliot, R., Evans, David John, Evans, D., Armstrong, V, Tuplin, Andrew, Evans, D.J., Struthers, Madeleine, Struthers, M., Elliott, Richard M., Diviney, S., Tuplin, A., Armstrong, V., Struthers, M, Simmonds, Peter, Elliott, Richard Michael, Armstrong, Victoria, Tuplin, A, Evans, David J., Simmonds, P., Diviney, S, Elliott, R.M., Simmonds, P   +22 more
core   +1 more source

A self-amplifying RNA vaccine against COVID-19 with long-term room-temperature stability

npj Vaccines, 2022
mRNA vaccines were the first to be authorized for use against SARS-CoV-2 and have since demonstrated high efficacy against serious illness and death. However, limitations in these vaccines have been recognized due to their requirement for cold storage ...
Emily A. Voigt, Alana Gerhardt, Derek Hanson, Madeleine F. Jennewein, Peter Battisti, Sierra Reed, Jasneet Singh, Raodoh Mohamath, Julie Bakken, Samuel Beaver, Christopher Press, Patrick Soon-Shiong, Christopher J. Paddon, Christopher B. Fox, Corey Casper   +14 more
doaj   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

An improved in vivo tethering assay with single molecule FISH reveals that a nematode Nanos enhances reporter expression and mRNA stability

, 2021
Robust methods are critical for testing the in vivo regulatory mechanism of RNA binding proteins. Here we report improvement of a protein–mRNA tethering assay to probe the function of an RNA binding protein in its natural context within the C.
Kimble, Judith, Lynch, Tina R., Aoki, Scott T., Doenier, Jonathan   +3 more
core   +1 more source

Neutrophil extracellular trap-associated RNA and LL37 enable self-amplifying inflammation in psoriasis

Nature Communications, 2020
Antimicrobial peptide LL37 can bind nucleic acids and potentiate their sensing by endosomal TLRs. Here the authors show that LL37 binds to RNA from neutrophil extracellular traps (NETs), which amplifies inflammation and production of more LL37 and NETs ...
Franziska Herster, Zsofia Bittner, Nathan K. Archer, Sabine Dickhöfer, David Eisel, Tatjana Eigenbrod, Thomas Knorpp, Nicole Schneiderhan-Marra, Markus W. Löffler, Hubert Kalbacher, Tim Vierbuchen, Holger Heine, Lloyd S. Miller, Dominik Hartl, Lukas Freund, Knut Schäkel, Martin Heister, Kamran Ghoreschi, Alexander N. R. Weber   +18 more
doaj   +1 more source

A phase I COVID-19 vaccine trial among SARS-CoV-2 seronegative and seropositive individuals in Uganda utilizing a self-amplifying RNA vaccine platform: Screening and enrollment experiences

Human Vaccines & Immunotherapeutics, 2023
We report the screening and enrollment process for a phase I vaccine trial in Masaka, Uganda that investigated the safety and immunogenicity of a self-amplifying SARS-CoV-2 RNA vaccine amongst individuals with and without antibodies to SARS-CoV-2 ...
Jonathan Kitonsa, Onesmus Kamacooko, Eugene Ruzagira, Florence Nambaziira, Andrew Abaasa, Jennifer Serwanga, Ben Gombe, Jane Lunkuse, Hadijah Naluyinda, Naboth Tukamwesiga, Tamara Namata, Antony Kigozi, Paddy Kafeero, Vincent Basajja, Sarah Joseph, Benjamin F. Pierce, Robin Shattock, Pontiano Kaleebu   +17 more
doaj   +1 more source