Results 31 to 40 of about 28,679 (139)

Perspectives on RNA Vaccine Candidates for COVID-19

Frontiers in Molecular Biosciences, 2021
With the current outbreak caused by SARS-CoV-2, vaccination is acclaimed as a public health care priority. Rapid genetic sequencing of SARS-CoV-2 has triggered the scientific community to search for effective vaccines.
Pobitra Borah, Pobitra Borah, Pran Kishore Deb, Nizar A. Al-Shar’i, Lina A. Dahabiyeh, Katharigatta N. Venugopala, Katharigatta N. Venugopala, Vinayak Singh, Vinayak Singh, Pottathil Shinu, Snawar Hussain, Satyendra Deka, Balakumar Chandrasekaran, Da’san M. M. Jaradat   +13 more
doaj   +1 more source

Expression Kinetics and Innate Immune Response after Electroporation and LNP-Mediated Delivery of a Self-Amplifying mRNA in the Skin

Molecular Therapy: Nucleic Acids, 2019
In this work, we studied the expression kinetics and innate immune response of a self-amplifying mRNA (sa-RNA) after electroporation and lipid-nanoparticle (LNP)-mediated delivery in the skin of mice. Intradermal electroporation of the sa-RNA resulted in
Hanne Huysmans, Zifu Zhong, Joyca De Temmerman, Barbara L. Mui, Ying K. Tam, Séan Mc Cafferty, Arlieke Gitsels, Daisy Vanrompay, Niek N. Sanders   +8 more
doaj   +1 more source

Self-Replicating RNA Viruses for Vaccine Development against Infectious Diseases and Cancer

Vaccines, 2021
Alphaviruses, flaviviruses, measles viruses and rhabdoviruses are enveloped single-stranded RNA viruses, which have been engineered for recombinant protein expression and vaccine development.
Kenneth Lundstrom
doaj   +1 more source

Heterologous vaccination regimens with self-amplifying RNA and adenoviral COVID vaccines induce robust immune responses in mice

Nature Communications, 2021
Heterologous vaccination regimens for COVID-19 could be useful for example if there is a shortage of one vaccine type. Here, Spencer et al. show that heterologous vaccination with a self-amplifying RNA vaccine and an adenoviral vectored vaccine performs ...
Alexandra J. Spencer, Paul F. McKay, Sandra Belij-Rammerstorfer, Marta Ulaszewska, Cameron D. Bissett, Kai Hu, Karnyart Samnuan, Anna K. Blakney, Daniel Wright, Hannah R. Sharpe, Ciaran Gilbride, Adam Truby, Elizabeth R. Allen, Sarah C. Gilbert, Robin J. Shattock, Teresa Lambe   +15 more
doaj   +1 more source

Improvement in the potency of a N¹-methylpseudouridine-modified self-amplifying RNA through mutations in the RNA-dependent RNA polymerase. [PDF]

J Biol Chem
ABSTRACT RNA vaccines are sensed as non-self molecules by the innate immune system, and balancing control of the immune activation and vaccine safety and efficacy has remained a challenge, especially for self-amplifying RNAs (saRNAs).
Quintana V, Caillava J, Byk LA, Mondotte JA, Battini L, Tarte P, Samsa MM, Filomatori CV, Alvarez DE.   +8 more
europepmc   +3 more sources

Self-Amplifying Replicon RNA Vaccine Delivery to Dendritic Cells by Synthetic Nanoparticles

Vaccines, 2014
Dendritic cells (DC) play essential roles determining efficacy of vaccine delivery with respect to immune defence development and regulation. This renders DCs important targets for vaccine delivery, particularly RNA vaccines.
Kenneth C. McCullough, Panagiota Milona, Lisa Thomann-Harwood, Thomas Démoulins, Pavlos Englezou, Rolf Suter, Nicolas Ruggli   +6 more
doaj   +1 more source

Enhanced Delivery and Potency of Self-Amplifying mRNA Vaccines by Electroporation in Situ

Vaccines, 2013
Nucleic acid-based vaccines such as viral vectors, plasmid DNA (pDNA), and mRNA are being developed as a means to address limitations of both live-attenuated and subunit vaccines.
Kaustuv Banerjee, Julie Hickman, Andrew Geall, Jeffrey B. Ulmer, Gleb Kichaev, Niranjan Y. Sardesai, Yen Cu, Susan Barnett, Gillis Otten, Kate E. Broderick   +9 more
doaj   +1 more source

Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine candidate induces high neutralizing antibody titers in mice

Nature Communications, 2020
Here, the authors develop a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle as a vaccine candidate and show induction of neutralization antibody titers in mice that are comparable to titers in ...
Paul F. McKay, Kai Hu, Anna K. Blakney, Karnyart Samnuan, Jonathan C. Brown, Rebecca Penn, Jie Zhou, Clément R. Bouton, Paul Rogers, Krunal Polra, Paulo J. C. Lin, Christopher Barbosa, Ying K. Tam, Wendy S. Barclay, Robin J. Shattock   +14 more
doaj   +1 more source

A new generation of vaccines based on alphavirus self-amplifying RNA

Current Opinion in Virology, 2020
DNA or mRNA vaccines have potential advantages over conventional vaccines since they are easier to manufacture and have higher safety profiles. In particular, self-amplifying RNA (saRNA) derived from alphavirus expression vectors has shown to be very efficient to induce humoral and cellular responses against many antigens in preclinical models, being ...
Ballesteros-Briones, María Cristina, Silva-Pilipich, Noelia, Herrador-Cañete, Guillermo, Vanrell, Lucia, Smerdou, Cristian   +4 more
openaire   +2 more sources

A phase I COVID-19 vaccine trial among SARS-CoV-2 seronegative and seropositive individuals in Uganda utilizing a self-amplifying RNA vaccine platform: Screening and enrollment experiences

Human Vaccines & Immunotherapeutics, 2023
We report the screening and enrollment process for a phase I vaccine trial in Masaka, Uganda that investigated the safety and immunogenicity of a self-amplifying SARS-CoV-2 RNA vaccine amongst individuals with and without antibodies to SARS-CoV-2 ...
Jonathan Kitonsa, Onesmus Kamacooko, Eugene Ruzagira, Florence Nambaziira, Andrew Abaasa, Jennifer Serwanga, Ben Gombe, Jane Lunkuse, Hadijah Naluyinda, Naboth Tukamwesiga, Tamara Namata, Antony Kigozi, Paddy Kafeero, Vincent Basajja, Sarah Joseph, Benjamin F. Pierce, Robin Shattock, Pontiano Kaleebu   +17 more
doaj   +1 more source