Results 21 to 30 of about 5,246,093 (238)

Efficient production of a self-amplifying RNA vaccine sequence with the single subunit RNA polymerase from <i>Klebsiella</i> phage 32 utilizing the native AU initiating dinucleotide. [PDF]

RNA
RNA polymerase (RNAP) is the enzyme that produces RNA for an increasingly large variety of uses. The need for efficient industrial production of high quantities of mRNA was exemplified during the SARS-CoV-2 pandemic.
Price MA, Trubitsyna M, Paget JE, Baker BW, Turner F, Rosser SJ.   +5 more
europepmc   +2 more sources

Conjugation of Mannans to Enhance the Potency of Liposome Nanoparticles for the Delivery of RNA Vaccines

Pharmaceutics, 2021
Recent approval of mRNA vaccines to combat COVID-19 have highlighted the potential of this platform. Lipid nanoparticles (LNP) is the delivery vehicle of choice for mRNA as they prevent its enzymatic degradation by encapsulation.
Roshan Goswami, Derek T. O’Hagan, Roberto Adamo, Barbara C. Baudner   +3 more
doaj   +1 more source

A Phase I/II Clinical Trial of Intradermal, Controllable Self-Replicating Ribonucleic Acid Vaccine EXG-5003 against SARS-CoV-2

Vaccines, 2023
mRNA vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have played a key role in reducing morbidity and mortality from coronavirus disease 2019 (COVID-19).
Takenao Koseki, Mayumi Teramachi, Minako Koga, Minoru S. H. Ko, Tomokazu Amano, Hong Yu, Misa Amano, Erica Leyder, Maria Badiola, Priyanka Ray, Jiyoung Kim, Akihiro C. Ko, Achouak Achour, Nan-ping Weng, Takumi Imai, Hisako Yoshida, Satsuki Taniuchi, Ayumi Shintani, Hidetsugu Fujigaki, Masashi Kondo, Yohei Doi   +20 more
doaj   +1 more source

Self-Amplifying RNA Approach for Protein Replacement Therapy. [PDF]

Int J Mol Sci, 2022
Messenger RNA (mRNA) technology has already been successfully tested preclinically and there are ongoing clinical trials for protein replacement purposes; however, more effort has been put into the development of prevention strategies against infectious ...
Papukashvili D, Rcheulishvili N, Liu C, Ji Y, He Y, Wang PG.   +5 more
europepmc   +2 more sources

Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism [PDF]

, 2010
For a long time it has been assumed that the decay of RNA in eukaryotes is mainly carried out by exoribonucleases, which is in contrast to bacteria, where endoribonucleases are well documented to initiate RNA degradation.
Tomecki, Rafał, Dziembowski, Andrzej
core   +3 more sources

Heterologous saRNA Prime, DNA Dual-Antigen Boost SARS-CoV-2 Vaccination Elicits Robust Cellular Immunogenicity and Cross-Variant Neutralizing Antibodies

Frontiers in Immunology, 2022
We assessed if immune responses are enhanced in CD-1 mice by heterologous vaccination with two different nucleic acid-based COVID-19 vaccines: a next-generation human adenovirus serotype 5 (hAd5)-vectored dual-antigen spike (S) and nucleocapsid (N ...
Adrian Rice, Mohit Verma, Emily Voigt, Peter Battisti, Sam Beaver, Sierra Reed, Kyle Dinkins, Shivani Mody, Lise Zakin, Shiho Tanaka, Brett Morimoto, C. Anders Olson, Elizabeth Gabitzsch, Jeffrey T. Safrit, Patricia Spilman, Corey Casper, Corey Casper, Patrick Soon-Shiong   +17 more
doaj   +1 more source

Advances in saRNA Vaccine Research against Emerging/Re-Emerging Viruses

Vaccines, 2023
Although conventional vaccine approaches have proven to be successful in preventing infectious diseases in past decades, for vaccine development against emerging/re-emerging viruses, one of the main challenges is rapid response in terms of design and ...
Yalan Liu, Yuncheng Li, Qinxue Hu
doaj   +1 more source

Perspectives on RNA Vaccine Candidates for COVID-19

Frontiers in Molecular Biosciences, 2021
With the current outbreak caused by SARS-CoV-2, vaccination is acclaimed as a public health care priority. Rapid genetic sequencing of SARS-CoV-2 has triggered the scientific community to search for effective vaccines.
Pobitra Borah, Pobitra Borah, Pran Kishore Deb, Nizar A. Al-Shar’i, Lina A. Dahabiyeh, Katharigatta N. Venugopala, Katharigatta N. Venugopala, Vinayak Singh, Vinayak Singh, Pottathil Shinu, Snawar Hussain, Satyendra Deka, Balakumar Chandrasekaran, Da’san M. M. Jaradat   +13 more
doaj   +1 more source

The role of nanoparticle format and route of administration on self-amplifying mRNA vaccine potency [PDF]

, 2022
The efficacy of RNA-based vaccines has been recently demonstrated, leading to the use of mRNA-based COVID-19 vaccines. The application of self-amplifying mRNA within these formulations may offer further enhancement to these vaccines, as self-amplifying ...
Baudner, Barbara C., Roberts, Craig W, Lou, Gustavo, Brazzoli, Michela, O'Hagan, Derek T., Gallorini, Simona, O'Hagan, Derek T, Woods, Stuart, Johnson, Russell, Roberts, Craig W., Baudner, Barbara C, Perrie, Yvonne, Schmidt, Signe Tandrup, Anderluzzi, Giulia   +13 more
core   +3 more sources

FACS purification and transcriptome analysis of drosophila neural stem cells reveals a role for Klumpfuss in self-renewal [PDF]

, 2012
Drosophila neuroblasts (NBs) have emerged as a model for stem cell biology that is ideal for genetic analysis but is limited by the lack of cell-type-specific gene expression data.
Jonas Steinmann, Heinrich Reichert, Reichert, Heinrich, Maria Novatchkova, Burkard, Thomas R., Laurenson, Anne-Sophie, Anne-Sophie Laurenson, Knoblich, Juergen A., Steinmann, Jonas, van der Horst, Suzanne, Berger, Christian, Novatchkova, Maria, Christian Berger, Juergen A. Knoblich, Thomas R. Burkard, Heike Harzer, Suzanne van der Horst, Harzer, Heike   +17 more
core   +1 more source