Results 71 to 80 of about 56,769 (258)

A unified design space of synthetic stripe-forming networks [PDF]

, 2014
Synthetic biology is a promising tool to study the function and properties of gene regulatory networks. Gene circuits with predefined behaviours have been successfully built and modelled, but largely on a case-by-case basis.
Cotterell, J, Gili, M, Isalan, M, Munteanu, A, Schaerli, Y, Sharpe, J   +5 more
core   +3 more sources

Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity

Molecular Oncology, EarlyView.
Peroxidasin (PXDN) is secreted by melanoma cells and binds the NK cell receptor NKG2D, thereby suppressing NK cell activation and cytotoxicity. PXDN depletion restores NKG2D signaling and enables effective NK cell–mediated melanoma killing. These findings identify PXDN as a previously unrecognized immune evasion factor and a potential target to improve
Hsu‐Min Sung, David Bickel, Lena C. M. Krause, Daria Ezeriņa, Christian Ickes, Julian Wojtachnia, Christine S. Gibhardt, Magdalena Shumanska, Khadija Wahni, Andrea Paluschkiwitz, Julia Malo Pueyo, Ekaterina Baranova, Wim Vranken, Hedwig Stanisz, Ioana Stejerean‐Todoran, Michael P. Schön, Joris Messens, Ivan Bogeski   +17 more
wiley   +1 more source

Ultra-compacted single self-amplifying RNA molecules as quintessential vaccines

, 2022
Abstract We introduce aqueous phases comprising single, highly compacted self-amplifying messenger RNA (saRNA) molecules, that are suitable for prophylactic and therapeutic application, specifically for vaccination against infectious diseases.
Jorge Moreno Herrero, Theo Stahl, Stephanie Erbar, Konrad Maxeiner, Anne Schlegel, Tijana Bacic, Leide Cavalcanti, Martin Schroer, Dmitri Svergun, Ugur Sahin, Heinrich Haas   +10 more
openaire   +1 more source

The Limitless Future of RNA Therapeutics

Frontiers in Bioengineering and Biotechnology, 2021
Recent advances in the generation, purification and cellular delivery of RNA have enabled development of RNA-based therapeutics for a broad array of applications.
Tulsi Ram Damase, Roman Sukhovershin, Christian Boada, Francesca Taraballi, Francesca Taraballi, Roderic I. Pettigrew, John P. Cooke   +6 more
doaj   +1 more source

The compositional and evolutionary logic of metabolism

, 2012
Metabolism displays striking and robust regularities in the forms of modularity and hierarchy, whose composition may be compactly described. This renders metabolic architecture comprehensible as a system, and suggests the order in which layers of that ...
Alberts B, Andreini C, Balche W E, Barker H A, Benner S A, Berge C, Braakman R, Brazelton W J, Buss L W, Collins M D, Copley S D, Dagley S, Darwin C, de Duve C, Doyle J, Eric Smith, Erwin D H, Feist A M, Fenchel T, Fisher R A, Fry I, Gerhart J, Gesteland R F, Gould S J, Gray H B, Guiral M, Haldane J B S, Kallen R G, Kauffman S, Kim J, Lengeler J W, Ljungdahl L, MacKenzie R E, Martin W, Massey L K, Metzler D E, Morowitz H J, Morowitz H J, Morowitz H J, Nelson D L, Nitscke W, Odling-Smee F J, Oparin A I, Petsko G A, Puigbo P, Ragsdale S, Ragsdale S W, Rankama K, Razin S, Redfield A C, Redfield A C, Rogier Braakman, Russell M J, Schrödinger E, Schuster P, Segré D, Shapiro R, Simon H A, Simon H A, Smith E, Srinivasan V, Srinivasan V, Sterner R W, Stryer L, Szent-Gyorgyi A, Tazuya K, Utter M F, Vogels G D, von Wettstein D, Vorholt J A, Waber L J, Wald G, Wilson E B, Wächtershäuser G, Yarus M, Yi L   +75 more
core   +1 more source

Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer

Molecular Oncology, EarlyView.
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova, Zuzana Kozovska, Ondrej Pos, Kristina Pavlov, Sachin Gulati, Peter Makovicky, Kristina Jakic, Monika Burikova, Eva Sedlackova, Barbora Svitkova, Silvia Tyciakova, Vojtech Bystry, Nicolas Blavet, Boris Tichy, Matej Hrnciar, Jaroslav Budis, Miroslav Tomas, Peter Dubovan, Georgina Kolnikova, Veronika Repaska, Nikoleta Mojzesova, Eva Zomborska, Daniel Pindak, Michal Mego, Tomas Szemes, Miroslava Matuskova   +25 more
wiley   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Perspective Chapter: Next-Generation Vaccines Based on Self-Amplifying RNA

, 2022
Recently, nucleic acid-based RNA and DNA vaccines have represented a better solution to avoid infectious diseases than “traditional” live and non-live vaccines. Synthetic RNA and DNA molecules allow scalable, rapid, and cell-free production of vaccines in response to an emerging disease such as the current COVID-19 pandemic.
Fatemeh, Nafian,, Simin, Nafian,, Ghazal, Soleymani,, Zahra, Pourmanouchehri,, Mahnaz, Kiyanjam,, Berenji, Jalaei, Sharareh, Hanie, Jeyroudi,, Mohammad, Mohammdi, Sayed   +7 more
openaire   +3 more sources

Dihydroorotate dehydrogenase (DHODH) regulates trophoblast syncytialization through organelle stress–induced cellular senescence

FEBS Open Bio, EarlyView.
The inhibition of mitochondrial dihydroorotate dehydrogenase (DHODH) impairs syncytialization and induces cellular senescence via mitochondrial and endoplasmic reticulum stress in human trophoblast stem cells, elevating sFlt1/PlGF levels, a hallmark of placental dysfunction in hypertensive disorders of pregnancy.
Kanoko Yoshida, Kazuya Kusama, Ayaka Horiuchi, Yu Kawaguchi, Atsuya Tsuru, Mikihiro Yoshie, Kazuhiro Tamura   +6 more
wiley   +1 more source

Plasticity of differentiated cells in wound repair and tumorigenesis, Part II: Skin and intestine [PDF]

, 2018
Recent studies have identified and begun to characterize the roles of regenerative cellular plasticity in many organs. In Part I of our two-part Review, we discussed how cells reprogram following injury to the stomach and pancreas.
Burclaff, Joseph, Mills, Jason C
core   +2 more sources