Results 11 to 20 of about 470,172 (244)

Megahertz serial crystallography [PDF]

open access: yesNature Communications, 2018
The new European X-Ray Free-Electron Laser (EuXFEL) is the first XFEL that generates X-ray pulses with a megahertz inter-pulse spacing. Here the authors demonstrate that high-quality and damage-free protein structures can be obtained with the currently ...
Max O. Wiedorn   +125 more
doaj   +18 more sources

Serial Crystallography: Preface [PDF]

open access: yesCrystals, 2020
The history of serial crystallography (SC) has its origins in the earliest attempts to merge data from several crystals. This preface provides an overview of some recent work, with a survey of the rapid advances made over the past decade in both sample ...
John C. H. Spence
doaj   +2 more sources

Macromolecular Serial Crystallography [PDF]

open access: yesCrystals, 2020
Within the structural biology field, X-ray crystallography prevails as the dominant technique to determine the structures of macromolecules, producing, as of November 2020, more than 150,000 structures since its inception (https://www [...]
Jose M. Martin-Garcia, Shibom Basu
doaj   +3 more sources

Serial X-ray Crystallography [PDF]

open access: yesCrystals, 2022
Serial crystallography (SX) is an emerging technique to determine macromolecules at room temperature. SX with a pump–probe experiment provides the time-resolved dynamics of target molecules.
Ki Hyun Nam
doaj   +2 more sources

Anaerobic fixed-target serial crystallography [PDF]

open access: yesIUCrJ, 2020
Cryogenic X-ray diffraction is a powerful tool for crystallographic studies on enzymes including oxygenases and oxidases. Amongst the benefits that cryo-conditions (usually employing a nitrogen cryo-stream at 100 K) enable, is data collection of dioxygen-
Patrick Rabe   +16 more
doaj   +5 more sources

Pink-beam serial crystallography [PDF]

open access: yesNature Communications, 2017
Serial X-ray crystallography (SX) is used for data collection at X-ray Free Electron Lasers. Here the authors show that a polychromatic “pink” synchrotron X-ray beam can be used for SX, which is useful when crystal supply is limited and will allow time ...
A. Meents   +19 more
doaj   +4 more sources

Approach of Serial Crystallography [PDF]

open access: yesCrystals, 2020
Radiation damage and cryogenic sample environment are an experimental limitation observed in the traditional X-ray crystallography technique. However, the serial crystallography (SX) technique not only helps to determine structures at room temperature ...
Ki Hyun Nam
doaj   +2 more sources

Comparative Structural Analysis of Escherichia Coli Cyay at Room and Cryogenic Temperatures Using Macromolecular and Serial Crystallography. [PDF]

open access: yesChembiochem
Frataxin is a 23 kDa mitochondrial iron‐binding protein involved in the biogenesis of iron–sulfur (Fe–S) clusters. Deficiency in frataxin is associated with Friedreich's ataxia, a progressive neurodegenerative disorder.
Shafiei A   +4 more
europepmc   +2 more sources

Serial X-ray Crystallography II

open access: yesCrystals, 2023
Traditional macromolecular crystallography (MX) and recently spotlighted cryogenic electron microscopy (Cryo-EM) techniques have contributed greatly to the development of macromolecule structures and the related fields [...]
Ki Hyun Nam
doaj   +2 more sources

Assessing the problem of excitation light scattering in high-viscosity jets used in serial crystallography sample delivery. [PDF]

open access: yesJ Appl Crystallogr
We experimentally demonstrate that the scattering-induced decrease in light intensity experienced by a crystal embedded in a high-viscosity jet is very limited in materials such as lipidic cubic phase or hydroxyethyl cellulose, and therefore cannot ...
Niziński S   +5 more
europepmc   +2 more sources

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