Results 191 to 200 of about 7,535 (227)

Room-temperature serial synchrotron crystallography of apo PTP1B

2022
AbstractRoom-temperature X-ray crystallography provides unique insights into protein conformational heterogeneity, but a common hurdle is obtaining sufficiently large protein crystals. Serial synchrotron crystallography (SSX) helps address this hurdle by allowing the use of many medium- to small-sized crystals. We have used a recently introduced serial
Shivani Sharma, Ali Ebrahim, Daniel A. Keedy   +2 more
openaire   +1 more source

Serial Synchrotron X-Ray Crystallography (SSX)

2017
Prompted by methodological advances in measurements with X-ray free electron lasers, it was realized in the last two years that traditional (or conventional) methods for data collection from crystals of macromolecular specimens can be complemented by synchrotron measurements on microcrystals that would individually not suffice for a complete data set ...
Kay, Diederichs, Meitian, Wang
openaire   +2 more sources

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

Journal of Visualized Experiments, 2020
A facility for performing serial crystallography measurements has been developed at the Australian synchrotron. This facility incorporates a purpose built high viscous injector, Lipidico, as part of the macromolecular crystallography (MX2) beamline to measure large numbers of small crystals at room temperature.
Peter, Berntsen, Rama, Sharma, Michael, Kusel, Brian, Abbey, Connie, Darmanin   +4 more
openaire   +2 more sources

Liquid application method for time-resolved analyses by serial synchrotron crystallography

Nature Methods, 2019
We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times.
Pedram Mehrabi, Eike C. Schulz, Michael Agthe, Sam Horrell, Gleb Bourenkov, David von Stetten, Jan-Philipp Leimkohl, Hendrik Schikora, Thomas R. Schneider, Arwen R. Pearson, Friedjof Tellkamp, R. J. Dwayne Miller   +11 more
openaire   +4 more sources

Processing serial synchrotron crystallography diffraction data with DIALS

This chapter describes additions to the DIALS software package for processing serial still-shot crystallographic data, and the implementation of a pipeline, xia2.ssx, for processing and merging serial crystallography data using DIALS programs. To integrate partial still-shot diffraction data, a 3D gaussian profile model was developed that can describe ...
James, Beilsten-Edmands, James M, Parkhurst, Graeme, Winter, Gwyndaf, Evans   +3 more
openaire   +2 more sources