Results 41 to 50 of about 7,535 (227)

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser. [PDF]

, 2015
G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways.
Barty, Anton, Basu, Shibom, Boutet, Sébastien, Caffrey, Martin, Caro, Lydia N, Carragher, Bridget, Chapman, Henry N, Cherezov, Vadim, Coe, Jesse, Conrad, Chelsie E, de Waal, Parker W, Diederichs, Kay, Dong, Yuhui, Ernst, Oliver P, Fromme, Petra, Fromme, Raimund, Gao, Xiang, Gati, Cornelius, Griffin, Patrick R, Grotjohann, Ingo, Gu, Xin, Gurevich, Vsevolod V, Han, Gye Won, He, Yuanzheng, Howe, Nicole, Hubbell, Wayne L, Ishchenko, Andrii, James, Daniel, Jiang, Hualiang, Jiang, Yi, Kang, Yanyong, Katritch, Vsevolod, Ke, Jiyuan, Kupitz, Christopher, Lee, Regina J, Li, Dianfan, Li, Jun, Lisova, Stella, Liu, Haiguang, Liu, Wei, Ma, Jinming, Melcher, Karsten, Messerschmidt, Marc, Moeller, Arne, Pal, Kuntal, Pascal, Bruce D, Potter, Clinton S, Roy-Chowdhury, Shatabdi, Spence, John CH, Standfuss, Jörg, Stevens, Raymond C, Suino-Powell, Kelly M, Tan, MH Eileen, Tan, Minjia, Van Eps, Ned, Vishnivetskiy, Sergey A, Wang, Dingjie, Wang, Meitian, Weierstall, Uwe, West, Graham M, White, Thomas A, Williams, Garth J, Xu, H Eric, Xu, Qingping, Yang, Huaiyu, Yefanov, Oleksandr, Zatsepin, Nadia A, Zhang, Chenghai, Zhao, Yingming, Zheng, Zhong, Zhi, Xiaoyong, Zhou, X Edward   +71 more
core   +1 more source

Serial synchrotron and XFEL crystallography for studies of metalloprotein catalysis

Current Opinion in Structural Biology, 2021
An estimated half of all proteins contain a metal, with these being essential for a tremendous variety of biological functions. X-ray crystallography is the major method for obtaining structures at high resolution of these metalloproteins, but there are considerable challenges to obtain intact structures due to the effects of radiation damage.
Hough, Michael A, Owen, Robin L
openaire   +3 more sources

Millisecond mix-and-quench crystallography (MMQX) enables time-resolved studies of PEPCK with remote data collection

IUCrJ, 2021
Time-resolved crystallography of biomolecules in action has advanced rapidly as methods for serial crystallography have improved, but the large number of crystals and the complex experimental infrastructure that are required remain serious obstacles to ...
Jonathan A. Clinger, David W. Moreau, Matthew J. McLeod, Todd Holyoak, Robert E. Thorne   +4 more
doaj   +1 more source

Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature

IUCrJ, 2022
Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, permits dynamic freedom in protein motions and enables time-resolved studies.
Tadeo Moreno-Chicano, Leiah M. Carey, Danny Axford, John H. Beale, R. Bruce Doak, Helen M. E. Duyvesteyn, Ali Ebrahim, Robert W. Henning, Diana C. F. Monteiro, Dean A. Myles, Shigeki Owada, Darren A. Sherrell, Megan L. Straw, Vukica Šrajer, Hiroshi Sugimoto, Kensuke Tono, Takehiko Tosha, Ivo Tews, Martin Trebbin, Richard W. Strange, Kevin L. Weiss, Jonathan A. R. Worrall, Flora Meilleur, Robin L. Owen, Reza A. Ghiladi, Michael A. Hough   +25 more
doaj   +1 more source

Proton uptake mechanism in bacteriorhodopsin captured by serial synchrotron crystallography [PDF]

Science, 2019
AbstractConformational dynamics are essential for proteins to function. Here we describe how we adapted time-resolved serial crystallography developed at X-ray lasers to visualize protein motions using synchrotrons. We recorded the structural changes upon proton pumping in bacteriorhodopsin over 200 ms in time.
Weinert, Tobias, Skopintsev, Petr, James, Daniel, Dworkowski, Florian, Panepucci, Ezequiel, Kekilli, Demet, Furrer, Antonia, Brünle, Steffen, Mous, Sandra, Ozerov, Dmitry, Nogly, Przemyslaw, Wang, Meitian, Standfuss, Jörg   +12 more
openaire   +2 more sources

High-speed fixed-target serial virus crystallography [PDF]

, 2017
We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with
Alonso-Mori, R, Brewster, AS, Damiani, DS, David, C, Duman, R, Fischer, P, Fry, EE, Ginn, HM, Harlos, K, Kelly, J, Kotecha, A, Meents, A, Meyer, J, Michels-Clark, T, Nelson, S, Pakendorf, T, Reime, B, Ren, J, Roedig, P, Rowlands, DJ, Sauter, NK, Sikorsky, M, Stuart, DI, Sutton, G, Vartiainen, I, Wagner, A, Walter, TS, Warmer, M, Young, ID   +28 more
core   +2 more sources

An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography. [PDF]

, 2015
SACLAのX線自由電子レーザーを用いた新規タンパク質立体構造決定に世界で初めて成功. 京都大学プレスリリース. 2015-09-14.Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) holds great potential for structure determination of challenging proteins that are not amenable to ...
Ago, Hideo, Gomi, Keiko, Hatsui, Takaki, Iwata, So, Joti, Yasumasa, Kajiyama, Naoki, Kameshima, Takashi, Kato, Hiroaki, Kodan, Atsushi, Mizohata, Eiichi, Murai, Tomohiro, Nakatsu, Toru, Nango, Eriko, Okuda, Tomohiko, Pan, Dongqing, Park, Jaehyun, Song, Changyong, Sugahara, Michihiro, Suzuki, Mamoru, Tono, Kensuke, Yabashi, Makina, Yamaguchi, Tomohiro, Yamamoto, Masaki, Yamashita, Keitaro   +23 more
core   +1 more source

A fixed-target platform for serial femtosecond crystallography in a hydrated environment. [PDF]

, 2020
For serial femtosecond crystallography at X-ray free-electron lasers, which entails collection of single-pulse diffraction patterns from a constantly refreshed supply of microcrystalline sample, delivery of the sample into the X-ray beam path while ...
Barthelmess, M, Batyuk, A, Coleman, MA, Evans, AC, Fischer, P, Frank, M, Gilbile, D, Grant, TD, He, W, Hunter, MS, Kuhl, TL, Meents, A, Pakendorf, T, Segelke, BW, Seuring, C, Shelby, ML   +15 more
core  

Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography

Structural Dynamics, 2015
We present a crystallography chip enabling in situ room temperature crystallography at microfocus synchrotron beamlines and X-ray free-electron laser (X-FEL) sources.
C. Mueller, A. Marx, S. W. Epp, Y. Zhong, A. Kuo, A. R. Balo, J. Soman, F. Schotte, H. T. Lemke, R. L. Owen, E. F. Pai, A. R. Pearson, J. S. Olson, P. A. Anfinrud, O. P. Ernst, R. J. Dwayne Miller   +15 more
doaj   +1 more source

Double-flow focused liquid injector for efficient serial femtosecond crystallography [PDF]

, 2017
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment.
Beyerlein, Kenneth R., Bushnell, David A., Knoska, Juraj, Kovaleva, Elena G. [u.v.m.], Oberthuer, Dominik, Szczepek, Michael, Wiedorn, Max O.   +6 more
core   +1 more source