Results 251 to 260 of about 865,522 (266)
Some of the next articles are maybe not open access.
Camelid single-domain antibodies raised by DNA immunization are potent inhibitors of EGFR signaling.
Biochemical Journal, 2019Up-regulation of epidermal growth factor receptor (EGFR) is a hallmark of many solid tumors, and inhibition of EGFR signaling by small molecules and antibodies has clear clinical benefit.
M. Rossotti +9 more
semanticscholar +1 more source
Expression of Single-Domain Antibodies in Bacterial Systems
2012In this chapter we describe in detail the current protocols that are used to express single-domain antibodies in bacteria. Bacteria are among the most common expression systems for expressing recombinant proteins. We present different approaches for carrying out periplasmic and cytoplasmic expression, as well as small-scale and large-scale expression ...
Baral, T.N., Arbabi-Ghahroudi, M.
openaire +3 more sources
Application of Single-Domain Antibodies in Tumor Histochemistry
2012High avidity, pentameric, single-domain antibodies, oligomerized through the B subunit of verotoxin, are excellent immunohistochemical reagents. The resulting molecules are termed pentabodies. Here, we describe the immunostaining of tissue sections with ES1, a pentabody recognizing CEACAM6 which is overexpressed in several cancers.
Maik, K.T., MacKenzie, C.R.
openaire +2 more sources
Expression of single-domain antibody in different systems
Applied Microbiology and Biotechnology, 2017Camelid single-domain antibodies (sdAbs, VHHs, or Nanobodies®) are types of antibody fragments that are composed of the heavy-chain variable domain only. These VHHs possess unique structural and functional features, as they are small in size and exhibit thermal stability and high solubility. Compared to conventional antibodies, VHHs can be manufactured
Yongkang, Liu, He, Huang
openaire +2 more sources
[Progress in shark single-domain antibody].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2020Monoclonal antibody (mAb) is an important biological macromolecule and widely used in immune detection, in vitro diagnostics, and drug discovery. However, the inherent properties of mAb restrict its further development, such as high molecular weight and complex structure. Therefore, there is an urgent need to develop alternatives for mAb. Various types
Xing, Liu, Qi, Chen
openaire +1 more source
Phage-Displayed Single Domain Antibodies as Recognition Elements
2013The variable domains of antibodies can be expressed as a fusion with pIII, a minor coat protein of the bateriophage M13, for the generation of phage-display antibody reagents. The phage-display system is routinely used to enrich for recombinant antibodies against a specific target antigen from highly diverse naïve and immune libraries.
Ellen, Goldman, Scott, Walper
openaire +2 more sources
2006
Classical antibodies are complex assemblies, difficult to produce in bacteria, and too bulky for many medical or research applications. Therefore, substantial efforts aimed at finding simpler miniaturised antibodies. Heavy-chain antibodies in camelids are a natural source of such mini-antibodies. Moreover, their antigen-binding domain (named Nanobody),
openaire +1 more source
Classical antibodies are complex assemblies, difficult to produce in bacteria, and too bulky for many medical or research applications. Therefore, substantial efforts aimed at finding simpler miniaturised antibodies. Heavy-chain antibodies in camelids are a natural source of such mini-antibodies. Moreover, their antigen-binding domain (named Nanobody),
openaire +1 more source
From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small
2012The development of therapeutic monoclonal antibodies over the last 35 years has led to the emergence of a new class of useful therapeutic molecules. These "first generation" antibodies have been obtained thanks to the conjugated and huge efforts of both academic and biotech researchers.
openaire +2 more sources
Camelid Single-Domain Antibodies for the Development of Potent Diagnosis Platforms
Molecular Diagnosis & Therapy, 2021Nairo Brilhante-da-Silva +7 more
semanticscholar +1 more source