Results 21 to 30 of about 594,568 (282)

Integrating Single Domain Antibodies into Field-Deployable Rapid Assays

Antibodies, 2022
Single domain antibodies (sdAb) are the recombinant variable heavy domains derived from camelid heavy-chain antibodies. While they have binding affinities equivalent to conventional antibodies, sdAb are only one-tenth the size and possess numerous ...
George P. Anderson, Lisa C. Shriver-Lake, Jinny L. Liu, Ellen R. Goldman   +3 more
doaj   +1 more source

Robust anti-SARS-CoV2 single domain antibodies cross neutralize multiple viruses

iScience, 2022
Summary: We report robust SARS-CoV2 neutralizing sdAbs targeting the viral peptides encompassing the polybasic cleavage site (CSP) and in the receptor binding domain (RBD) of the spike (S) protein.
Sudhakar Singh, Surbhi Dahiya, Yuviana J. Singh, Komal Beeton, Ayush Jain, Roman Sarkar, Abhishek Dubey, Azeez Tehseen, Sharvan Sehrawat   +8 more
doaj   +1 more source

Llama-Derived Single Domain Antibodies to Build Multivalent, Superpotent and Broadened Neutralizing Anti-Viral Molecules [PDF]

, 2011
For efficient prevention of viral infections and cross protection, simultaneous targeting of multiple viral epitopes is a powerful strategy. Llama heavy chain antibody fragments (VHH) against the trimeric envelope proteins of Respiratory Syncytial Virus (
Schepens, B, Temperton, Nigel J., M. Koenders, C. Theo Verrips, Schillemans, J, Ibanez, Lorena Itatı, J. Melero, de Haard, HJ, Verrips, C. Theo, S. Van Gucht, C.T. Verrips, Michael Saunders, Bert Schepens, Temperton, Nigel J, Ibanez, Lorena, Koenders, M, Melero, Jose A., Schepens Bert, de Haard, Hans J, Vanlandschoot, P, Steven Van Gucht, Gonzalez-Pajuelo Maria, Van Gucht, S, Xavier Saelens, Vanlandschoot Peter, Anna Hultberg, B. Schepens, Gonzalez-Pajuelo, Maria, Schillemans Joris, Koenders, Mireille, Nigel J. Temperton, Saunders Michael, Melero José A., de Haard, Hans J., Schillemans, Joris, Ibañez Lorena Itatí, de Haard Hans J., Temperton Nigel J., Ibañez,, Lorena Itatí, H.J. de Haard, Robin A. Weiss, Hultberg, A, Schepens, Bert, C Theo Verrips, N.J. Temperton, Ibanez, LI, Van Gucht, Steven, Verrips, Theo, Maria Gonzalez-Pajuelo, L.I. Ibanez, Rosseels Valérie, José A Melero, Hans J de Haard, Mireille Koenders, Hultberg Anna, Weiss, RA, Saelens Xavier, Saunders, Michael, R.A. Weiss, Weiss Robin A., Saelens, Xavier, Hans J. de Haard, Lorena Itatí Ibañez, A. Hultberg, Gonzalez-Pajuelo, M, Weiss, Robin A., Saelens, X, Joris Schillemans, Verrips, CT, Peter Vanlandschoot, Van Gucht Steven, M. Saunders, Valérie Rosseels, V. Rosseels, Koenders Mireille, Robin A Weiss, P. Vanlandschoot, Rosseels, V, José A. Melero, Hultberg, Anna, M. Gonzalez-Pajuelo, Rosseels, Valerie, J. Schillemans, Ibañez, Lorena Itatí, Melero, Jose Antonio, Vanlandschoot, Peter, Weiss, Robin A, Saunders, M, Nigel J Temperton, X. Saelens, Melero, José A, Verrips C. Theo, Temperton, NJ, Melero, JA, Verrips, C Theo   +94 more
core   +1 more source

Single‐Domain Antibodies and Their Utility

Current Protocols in Immunology, 2013
AbstractEngineered monoclonal antibody fragments have gained market attention due to their versatility and tailor‐made potential and are now considered to be an important part of future immunobiotherapeutics. Single‐domain antibodies (sdAbs), also known as nanobodies, are derived from VHHs [variable domains (V) of heavy‐chain‐only antibodies (HCAb)] of
Baral, Toya Nath, MacKenzie, Roger, Ghahroudi, Mehdi Arbabi   +2 more
openaire   +4 more sources

Bglbrick strategy for the construction of single domain antibody fusions

Heliyon, 2017
Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications.
Ellen R. Goldman, Aeris Broussard, George P. Anderson, Jinny L. Liu   +3 more
doaj   +1 more source

Single-domain antibody screening by isPLA-seq [PDF]

Life Science Alliance, 2021
Single-domain antibody (sdAb) holds the promising strategies for diverse research and translational applications. Here, we describe a method for the adaptation of the in situ proximity ligation assay (isPLA) followed by sequencing (isPLA-seq) to facilitate screening of a high-sensitive, high-throughput sdAb library for a given protein at subcellular ...
Yueyuan Yin, Fei Yan, Ruimin Zhou, Mingchen Li, Jinyi Ma, Zhe Liu, Zhenyi Ma   +6 more
openaire   +2 more sources

Expression of recombinant multi-coloured fluorescent antibodies in gor ^-/trxB^- E. coli cytoplasm [PDF]

, 2011
Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V., Burchell, Joy Marilyn; id_orcid, Kang, Angray S., Anatoliy Markiv, Burchell, J, Durvasula, R.V., Beatson, R., Beatson Richard, Beatson, Richard; id_orcid, Kang Angray S, Markiv, A., Markiv Anatoliy, Kang, A.S., Richard Beatson, Burchell, J., Durvasula Ravi V, Ravi V Durvasula, Burchell, Joy; id_orcid, Durvasula, RV, Markiv, Anatoliy, Burchell Joy, Markiv, A, Angray S Kang, Beatson, Richard, Joy Burchell, Kang, AS, Beatson, R   +26 more
core   +1 more source

NANOBODIES: STRUCTURE, MANUFACTURING, APPLICATION (REVIEW)

Разработка и регистрация лекарственных средств, 2019
Introduction. Single-domain antibodies (nanobodies) are composed of the heavy-chain variable domain only. Compared to conventional immunoglobulins G (IgG) nanobodies have such qualities as: high bioavailability, ability to bind epitopes that are ...
A. V. Shatalova, A. S. Yakubova, V. V. Palimpsestov, I. B. Esmagambetov   +3 more
doaj   +1 more source

Human monoclonal antibodies to a novel cluster of conformational epitopes on HCV E2 with resistance to neutralization escape in a genotype 2a isolate [PDF]

, 2012
The majority of broadly neutralizing antibodies to hepatitis C virus (HCV) are against conformational epitopes on the E2 glycoprotein. Many of them recognize overlapping epitopes in a cluster, designated as antigenic domain B, that contains residues G530
Wenyan Wang (171659), Bukh, J., Felix A Rey, Yong Wang (12837), Wang, Yong, Zhen-yong Keck (171653), Jannick Prentoe (162323), Lemon, S.M., Jannick Prentoe, Jens Bukh (162343), Steven K. H. Foung (171667), Lemon, Stanley M., Prentoe Jannick, Foung Steven K. H., Thomas Carlsen (171662), Carlsen, Thomas, Rey, F.A., Patel, Arvind H., Li, A.Y., Patel, A.H., Steven K H Foung, Carlsen Thomas, Bukh, Jens, Xia, J., Zhen-yong Keck, Li, Angela Ying-Jian, Wenyan Wang, Keck, Zhen-yong, Thomas Krey, Wang, Y., Rey Felix A., Yong Wang, Foung, S.K.H., Arvind H. Patel (33806), Patel, Arvind H, Lemon, Stanley M, Krey Thomas, Keck Zhen-yong, Wang Wenyan, Rey, Felix A., Prentoe, Jannick, Arvind H Patel, Bukh Jens, Stanley M Lemon, Krey, T., Angela Ying-Jian Li, Felix A. Rey (171664), Foung, Steven K H, Thomas Krey (171661), Keck, Z., Xia Jinming, Krey, Thomas, Jinming Xia (171656), Stanley M. Lemon (30543), Wang, Wenyan, Lemon Stanley M., Patel Arvind H., Rey, Felix A, Li Angela Ying-Jian, Jinming Xia, Xia, Jinming, Foung, Steven K. H., Thomas Carlsen, Wang, W., Prentoe, J., Jens Bukh, Angela Ying-Jian Li (171663), Carlsen, T., Wang Yong   +68 more
core   +1 more source

Llama-Derived Single Domain Antibodies Specific for Abrus Agglutinin

Toxins, 2011
Llama derived single domain antibodies (sdAb), the recombinantly expressed variable heavy domains from the unique heavy-chain only antibodies of camelids, were isolated from a library derived from llamas immunized with a commercial abrin toxoid ...
James P. Carney, Rachael Bernstein, Eric A. E. Garber, Jennifer L. Walker, Thomas W. O’Brien, Alena Calm, Jinny L. Liu, Ellen R. Goldman, Scott Walper, Dan Zabetakis, George P. Anderson   +10 more
doaj   +1 more source