Results 11 to 20 of about 628,463 (260)

Therapeutic Phage Display-Derived Single-Domain Antibodies for Pandemic Preparedness

Antibodies, 2023
Driven by necessity, the COVID-19 pandemic caused by SARS-CoV-2 has accelerated the development and implementation of new vaccine platforms and other viral therapeutics. Among these is the therapeutic use of antibodies including single-domain antibodies,
Janet M. Daly, Theam Soon Lim, Kevin C. Gough   +2 more
doaj   +1 more source

Stabilization of a Broadly Neutralizing Anti-Chikungunya Virus Single Domain Antibody

Frontiers in Medicine, 2021
A single domain antibody (clone CC3) previously found to neutralize a vaccine strain of the chikungunya virus (PRNT50 = 2. 5 ng/mL) was found to be broadly neutralizing.
Jinny L. Liu, Emily M. Webb, Dan Zabetakis, Crystal W. Burke, Christina L. Gardner, Pamela J. Glass, Patricia M. Legler, James Weger-Lucarelli, George P. Anderson, Ellen R. Goldman   +9 more
doaj   +1 more source

Single‐Domain Antibodies and Their Utility

Current Protocols in Immunology, 2013
AbstractEngineered monoclonal antibody fragments have gained market attention due to their versatility and tailor‐made potential and are now considered to be an important part of future immunobiotherapeutics. Single‐domain antibodies (sdAbs), also known as nanobodies, are derived from VHHs [variable domains (V) of heavy‐chain‐only antibodies (HCAb)] of
Baral, Toya Nath, MacKenzie, Roger, Ghahroudi, Mehdi Arbabi   +2 more
openaire   +4 more sources

Structure of a VHH isolated from a naïve phage display library

BMC Research Notes, 2019
Objective To determine the X-ray structure and biophysical properties of a Camelid VHH isolated from a naïve phage display library. Results Single domain antibodies (VHH) derived from the unique immune system of the Camelidae family have gained traction ...
Brandy White, Ian Huh, Cory L. Brooks
doaj   +1 more source

Bglbrick strategy for the construction of single domain antibody fusions

Heliyon, 2017
Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications.
Ellen R. Goldman, Aeris Broussard, George P. Anderson, Jinny L. Liu   +3 more
doaj   +1 more source

mRNA Encoding a Bispecific Single Domain Antibody Construct Protects against Influenza A Virus Infection in Mice

Molecular Therapy: Nucleic Acids, 2020
To date, mRNA-based biologics have mainly been developed for prophylactic and therapeutic vaccination to combat infectious diseases or cancer. In the past years, optimization of the characteristics of in vitro transcribed mRNA has led to significant ...
Lien Van Hoecke, Rein Verbeke, Dorien De Vlieger, Heleen Dewitte, Kenny Roose, Sharon Van Nevel, Olga Krysko, Claus Bachert, Bert Schepens, Ine Lentacker, Xavier Saelens   +10 more
doaj   +1 more source

A single domain antibody-based Luminex assay for the detection of SARS-CoV-2 in clinical samples. [PDF]

Front Immunol
Goldman ER, Sugiharto VA, Shriver-Lake LC, Garcia AM, Wu SJ, Jenkins SA, Chen HW.   +6 more
europepmc   +2 more sources

NbX: Machine Learning-Guided Re-Ranking of Nanobody–Antigen Binding Poses

Pharmaceuticals, 2021
Modeling the binding pose of an antibody is a prerequisite to structure-based affinity maturation and design. Without knowing a reliable binding pose, the subsequent structural simulation is largely futile.
Chunlai Tam, Ashutosh Kumar, Kam Y. J. Zhang   +2 more
doaj   +1 more source

Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions

Biotechnology Reports, 2016
Straightforward and effective methods are required for the bioconjugation of proteins to surfaces and particles. Previously we demonstrated that the fusion of a single domain antibody with the biotin binding molecule rhizavidin provided a facile method ...
Jinny L. Liu, Scott A. Walper, Kendrick B. Turner, Audrey Brozozog Lee, Igor L. Medintz, Kimihiro Susumu, Eunkeu Oh, Dan Zabetakis, Ellen R. Goldman, George P. Anderson   +9 more
doaj   +1 more source

A comparison of the binding sites of antibodies and single-domain antibodies

Frontiers in Immunology, 2023
Antibodies are the largest class of biotherapeutics. However, in recent years, single-domain antibodies have gained traction due to their smaller size and comparable binding affinity. Antibodies (Abs) and single-domain antibodies (sdAbs) differ in the structures of their binding sites: most significantly, single-domain antibodies lack a light chain and
Gemma L. Gordon, Henriette L. Capel, Bora Guloglu, Eve Richardson, Ryan L. Stafford, Charlotte M. Deane   +5 more
openaire   +3 more sources