Results 61 to 70 of about 10,437 (162)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Premature Sister Chromatid Separation Is Poorly Detected by the Spindle Assembly Checkpoint as a Result of System-Level Feedback

Cell Reports, 2015
Sister chromatid cohesion, mediated by the cohesin complex, is essential for faithful mitosis. Nevertheless, evidence suggests that the surveillance mechanism that governs mitotic fidelity, the spindle assembly checkpoint (SAC), is not robust enough to ...
Mihailo Mirkovic, Lukas H. Hutter, Béla Novák, Raquel A. Oliveira   +3 more
doaj   +1 more source

Multi‐Omics Profiling Reveals Immunomodulatory and Pro‐Regenerative Effects of a Graphene Oxide–Collagen Scaffold in Massive Rotator Cuff Tears

Advanced Science, EarlyView.
A graphene oxide/collagen scaffold is developed for chronic massive rotator cuff tear repair. The scaffold improves compressive stability, supports reparative mesenchymal differentiation, and modulates the immune microenvironment. In chronic MRCT models, it reduces muscle degeneration, enhances tendon–bone regeneration, and improves functional recovery,
Renwen Wan, Yechuan Deng, Zixin Hu, Yanwei He, Xinting Feng, Jun Ma, Yisheng Chen, Zhijie Zhao, Jie Mei, Zhiheng Lin, Wei Luo, Zhengyuan Fang, Zhufeng Hu, Kunlun Feng, Xinrong Li, Dan Wu, Bowen Han, James Hoi po Hui, Xuanyong Liu, Chen Chen, Gang Chen, Shiyi Chen, Nirong Bao, Jiajun Qiu, Zhiwen Luo   +24 more
wiley   +1 more source

Cohesin: Functions beyond sister chromatid cohesion [PDF]

FEBS Letters, 2013
Faithful segregation of chromosomes during mitosis and meiosis is the cornerstone process of life. Cohesin, a multi‐protein complex conserved from yeast to human, plays a crucial role in this process by keeping the sister chromatids together from S‐phase to anaphase onset during mitosis and meiosis.
Mehta, Gunjan D., Kumar, Ravinder, Srivastava, Sanjeeva, Ghosh, Santanu Kumar   +3 more
openaire   +2 more sources

Replication factor C complexes play unique pro- and anti-establishment roles in sister chromatid cohesion. [PDF]

PLoS ONE, 2010
Recent studies have lead to a rapid expansion of sister chromatid cohesion pathways. Of particular interest is the growth in classifications of anti-establishment factors-now including those that are cohesin-associated (Rad61/WAPL and Pds5) or DNA ...
Marie E Maradeo, Robert V Skibbens
doaj   +1 more source

Equine models in translational medicine: A comparative approach to human health

Animal Models and Experimental Medicine, EarlyView.
This diagram summarizes and contrasts rodent and equine models, outlining their strengths, limitations, and applications. Horses offer naturally occurring diseases, genetic and physiological similarities to humans, and suitability for longitudinal and clinical‐scale studies.
Shayan Boozarjomehri Amnieh, Katarzyna Ropka‐Molik   +1 more
wiley   +1 more source

Mutation type‐specific transcriptomic signatures and readthrough therapy rescue in SMC1A‐related developmental and epileptic encephalopathy

Epilepsia, EarlyView.
Abstract Objective This study was undertaken to investigate the molecular consequences of pathogenic variants in the SMC1A gene—particularly those associated with developmental and epileptic encephalopathy (DEE85)—and to evaluate the therapeutic potential of ataluren in restoring SMC1A function and mitigating disease‐related transcriptomic and genomic ...
Maddalena Di Nardo, Francesca Sardina, Maria M. Pallotta, Iñigo Marcos‐Alcalde, Paulino Gómez‐Puertas, Cinzia Rinaldo, Ian D. Krantz, Antonio Musio   +7 more
wiley   +1 more source

Chl1 DNA helicase regulates Scc2 deposition specifically during DNA-replication in Saccharomyces cerevisiae.

PLoS ONE, 2013
The conserved family of cohesin proteins that mediate sister chromatid cohesion requires Scc2, Scc4 for chromatin-association and Eco1/Ctf7 for conversion to a tethering competent state.
Soumya Rudra, Robert V Skibbens
doaj   +1 more source

A Long Noncoding RNA Regulates Sister Chromatid Cohesion. [PDF]

Mol Cell, 2016
Long noncoding RNAs (lncRNAs) are involved in diverse cellular processes through multiple mechanisms. Here, we describe a previously uncharacterized human lncRNA, CONCR (cohesion regulator noncoding RNA), that is transcriptionally activated by MYC and is upregulated in multiple cancer types.
Marchese FP, Grossi E, Marín-Béjar O, Bharti SK, Raimondi I, González J, Martínez-Herrera DJ, Athie A, Amadoz A, Brosh RM, Huarte M.   +10 more
europepmc   +8 more sources

Silencing SGO2 by Oxamic Acid Dissociates Glycolysis and BRCA1‐Mediated DNA Repair to Improve the Chemosensitivity of Lung Adenocarcinoma

Exploration, EarlyView.
SGO2 interacts with BRCA1 to inhibit BRCA1 ubiquitination and degradation, thereby promoting BRCA1‐induced DNA damage repair signaling and reducing the chemo sensitivity of LUADa. OA targets glycolysis to disrupt H3K18la‐ and H3K27ac‐mediated chromatin accessibility, repressing SGO2 transcription and subsequently alleviating SGO2‐mediated cancer ...
Xian Lin, Zhidan Hua, Chen Liu, Minxia Yang, Beilei Zhang, Xiaofeng Zhu, Xiao Chen   +6 more
wiley   +1 more source