Results 101 to 110 of about 354,238 (282)

Effects of mutations in the helix G region of horseradish peroxidase [PDF]

, 2008
Horseradish peroxidase (HRP) has long attracted intense research interest and is used in many biotechnological fields, including diagnostics, biosensors and biocatalysis.
Barry J. Ryan, Batra, Chakrabarti, Chang, Chattopadhyay, Ciarán Ó'Fágáin, Coates, Dai, Dunford, French, Gajhede, Garcia, Gonera, Gorman, Grigorenko, Guex, Hakamada, Haque, Hassani, Howes, Ignatenko, Jager, Kaposi, Khmelnitsky, Krowarsch, Kwon, Lei, Lin, Lipovsek, Liu, Liu, Machado, Miland, Miland, Mogharrab, Moosavi-Movahedi, Morawski, Nagano, Numata, O'Brien, O'Brien, Orlova, Ozaki, Pina, Priori, Ryan, Ryan, Ryan, Ryu, Sambrook, Santucci, Savenkova, Smeller, Smith, Tams, Tanaka, Tanaka, Ugarova, Urrutigoity, Wang   +59 more
core   +1 more source

A Cold Stress‐Activated Endocrine Sentinel Chemical Hormone Promotes Insect Survival via Mitochondrial Adaptations Through the Adipokinetic Hormone Receptor

Advanced Science, EarlyView.
Seasonal cold adaptation is vital for insect survival, yet the molecular mechanisms linking diapause to mitochondrial resilience remain largely unresolved. We identify ascaroside C9 (asc‐C9) as a key endocrine signal that enhances diapause survival during cold stress by activating the AKHR–PGC1α–UCP4 axis, thereby driving cold‐induced lipolysis and ...
Jiao Zhou, Junxian Chen, Hongxia Zhang, Lu Guo, Kevin Mai, Elijah Abraham, Dilip V. Prajapati, Yue Chang, Jing Ning, Xinchen Wang, Defeng Li, Dan‐Dan Zhang, Jianghua Sun, Rebecca A. Butcher, Lilin Zhao   +14 more
wiley   +1 more source

Antimicrobial characterization of site-directed mutagenesis of porcine beta defensin 2.

PLoS ONE, 2015
Porcine β defensin 2 (pBD2) is a small, cationic and amphiphilic antimicrobial peptide. It has broad antimicrobial activities against bacteria and plays an important role in host defense.
Xian-xian Huang, Chun-yu Gao, Qing-jun Zhao, Chun-li Li   +3 more
doaj   +1 more source

Cinnamic‐Hydroxamic‐Acid Derivatives Exhibit Antibiotic, Anti‐Biofilm, and Supercoiling Relaxation Properties by Targeting Bacterial Nucleoid‐Associated Protein HU

Advanced Science, EarlyView.
Cinnamic‐hydroxamic‐acid derivatives (CHADs) are identified as novel inhibitors of the bacterial nucleoid‐associated protein HU, exhibiting potent antibacterial, anti‐biofilm (both inhibition and eradication), and DNA relaxation (anti‐supercoiling) activities. Moreover, CHADs demonstrate strong synergistic effects with multiple antibiotics.
Huan Chen, Yingqi Xu, Zhaoping Xiong, Hongliang Wang, Xin Wang, Yue Kang, Zhonglin Wang, Xiaoyan Zeng, Yahui Liu, Yehuan Zheng, Wei Chen, Mengzhe Li, Zhenyue Hu, Chi Xu, Yue Wu, Yawen Wang, Zuyi Yuan, Shuai Yuan, Huadong Liu, Steve Matthews, Nan Qiao, Yan Li, Bing Liu   +22 more
wiley   +1 more source

Scarless and site-directed mutagenesis in Salmonella enteritidis chromosome

BMC Biotechnology, 2007
Background A variety of techniques have been described which introduce scarless, site-specific chromosomal mutations. These techniques can be applied to make point mutations or gene deletions as well as insert heterologous DNA into bacterial vectors for ...
Berghman Luc R, Hargis Billy M, Cole Kim, Jiang Tieshan, Layton Sherryll L, Cox Mandy M, Bottje Walter G, Kwon Young   +7 more
doaj   +1 more source

TBK1 Induces the Formation of Optineurin Filaments That Condensate with Polyubiquitin and LC3 for Cargo Sequestration

Advanced Science, EarlyView.
Phosphorylation of Optineurin by TBK1 induces the formation of filaments that condensate upon binding to linear polyubiquitin. Membrane‐anchored LC3 partitions into these condensates, suggesting that phase separation of filamentous Optineurin with ubiquitylated cargo promotes the sequestration of cargo and its subsequent alignment with LC3‐positive ...
Maria G. Herrera, Lena Kühn, Lisa Jungbluth, Verian Bader, Laura J. Krause, David Kartte, Elias Adriaenssens, Sascha Martens, Jörg Tatzelt, Carsten Sachse, Konstanze F. Winklhofer   +10 more
wiley   +1 more source

Lactylation‐Driven YTHDC1 Alleviates MASLD by Suppressing PTPN22‐Mediated Dephosphorylation of NLRP3

Advanced Science, EarlyView.
In MASLD, YTHDC1 undergoes increased lactylation and ubiquitination, reducing its expression. AARS1 mediates lactylation at lysine 565, while disrupted binding to LDHA further promotes lactylation, suppressing YTHDC1. This downregulation enhances PTPN22 mRNA stability, leading to NLRP3 dephosphorylation and activation, which exacerbates inflammation ...
Feng Zhang, Linghua Zeng, Kunkun Zou, Keying Lin, Fangting Yao, Jinglun Song, Shumeng Zhang, Hanshu Feng, Zhichao Yang, Chunlei Wang, Hongtao Diao, Xue Kong, Tengfei Pan, Joonki Kim, Tianqi Duo, Linqiang Li, Yu Bian   +16 more
wiley   +1 more source

Exploring Important Protein Interactions in the Mammalian Diaphanous-related Formins: A Closer Look at the Relationship between the Intracellular Skeletal Network and a Single Amino Acid Residue [PDF]

, 2006
The Diaphanous-related formin protein family plays a key role in intracellular cytoskeletal regulation. Due to their farreaching importance, these proteins must be highly regulated themselves.
Hehl, Brent
core   +2 more sources

site-directed mutagenesis

Citation: 'site-directed mutagenesis' in the IUPAC Compendium of Chemical Terminology, 5th ed.; International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.12726 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms ...
openaire   +1 more source

Balanced Expression of the Diiron Oxygenase BioE Is Essential for Biotin Homeostasis in Elizabethkingia meningoseptica

Advanced Science, EarlyView.
BioE is a new diiron oxygenase that catalyzes the conversion of long‐chain acyl groups into pimeloyl thioester, initiating biotin synthesis. The overexpression of EmBioE disrupts lipid metabolic homeostasis, requiring repressor BioL to maintain a balance between long‐chain fatty acids and biotin synthesis.
Meng Zhang, Ying‐ying Fu, Xiaoqiang Yang, Qiuying Qin, Xinyu Su, Jiaming Fang, Yanhua Kang, Qingwen He, Zhi Ruan, Yongchang Xu   +9 more
wiley   +1 more source