Results 31 to 40 of about 347,258 (205)

Construction of Glycine Oxidase Mutant Libraries by Random Mutagenesis, Site Directed Mutagenesis and DNA Shuffling

Bio-Protocol, 2014
Glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (GO) has been reported to be able to catalyze the oxidative ...
Tao Zhan
doaj   +1 more source

A membrane-inserted structural model of the yeast mitofusin Fzo1 [PDF]

, 2017
Mitofusins are large transmembrane GTPases of the dynamin-related protein family, and are required for the tethering and fusion of mitochondrial outer membranes.
A Fiser, A Franco, A Lupas, A Sali, A Santel, AA Polyansky, AB Knott, AM Bertholet, B Brosig, B Hess, B Westermann, C Notredame, C Volland, CR Søndergaard, D Naon, D Rapaport, DT Jones, DT Jones, E Boutet, EE Griffin, EF Pettersen, EG Hutchinson, F Anton, F Anton, F Armougom, F Sievers, G Pollastri, GJ Hermann, H Kishida, H Sesaki, H Viklund, HH Low, HH Low, HJC Berendsen, HM Berman, IM Wallace, JM Mason, JS Chappie, K Hofmann, K Labbé, L Cavellini, L Yan, L Yan, M Escobar-Henriques, M Rojo, M-P Durrieu, M-Y Shen, MA Lemmon, MA Lomize, MM Cohen, MS Longtine, N Ishihara, NJ Gleason, O Daumke, OM Brito de, P Hubert, R Filadi, R Yan, RA Laskowski, RA Laskowski, RFS Walters, RL Dunbrack, S Fritz, S Jo, S Lee, S-Q Zhang, SF Altschul, T Darden, T Koshiba, T Nugent, T Shutt, TJ Dolinsky, VB Chen, W Kabsch, WL Jorgensen, X Bian, X Daura, Y Qi, Y-L Cao   +78 more
core   +4 more sources

Highly Efficient Site-Specific and Cassette Mutagenesis of Plasmids Harboring GC-Rich Sequences

Cells
GC-rich sequences affect DNA replication, recombination and repair, as well as RNA transcription in vivo. Such sequences may also impede site-directed mutagenesis in vitro.
Paulina Varela-Castillo, Ethan Zhou, Arezousadat Razavi, Elham Ebrahimi, Xiang-Jiao Yang   +4 more
doaj   +1 more source

Effect of substitution of Cys11 by Ser on the activity and dimerization of one of rice thioredoxin isoforms (OsTrx23) using site-directed mutagenesis [PDF]

مجله بیوتکنولوژی کشاورزی, 2015
Thioredoxins (Trxs) are low-molecular-mass proteins with two cysteins in their active site WC(G/P)PC which are involved in reversible reduction of disulfide bonds.
Mitra Roudgar Nashta, Azar Shah Piri
doaj   +1 more source

A combined computational-experimental approach to define the structural origin of antibody recognition of sialyl-Tn, a tumor-associated carbohydrate antigen. [PDF]

, 2018
Anti-carbohydrate monoclonal antibodies (mAbs) hold great promise as cancer therapeutics and diagnostics. However, their specificity can be mixed, and detailed characterization is problematic, because antibody-glycan complexes are challenging to ...
Amon, Ron, Chen, Xi, Fleishman, Sarel J, Glushka, John N, Grant, Oliver C, Leviatan Ben-Arye, Shani, Makeneni, Spandana, Marshanski, Tal, Nivedha, Anita K, Norn, Christoffer, Padler-Karavani, Vered, Woods, Robert J, Yu, Hai   +12 more
core   +2 more sources

The kinetics of folding of the NSH2 domain from p85 [PDF]

, 2019
SH2 domains are protein domains that mediate protein-protein interaction through the recognition and binding of specific sequences containing phosphorylated tyrosines.
Gianni, Stefano, Malagrinò, Francesca, Toto, Angelo, Visconti, Lorenzo   +3 more
core   +2 more sources

Site-Directed Mutagenesis

, 1983
In the preceding chapters dealing with modifications of nucleic acids it is assumed that, in general, these chemical reactions are randomly located along the genome. Exceptions occur when protein-nucleic interactions or nucleic acid secondary structure playa role.
B. Singer, D. Grunberger
openaire   +3 more sources

High-throughput site-directed mutagenesis using oligonucleotides synthesized on DNA chips

BioTechniques, 2005
Site-directed mutagenesis has greatly helped researchers both to understand the precise role of specific residues in coding sequences and to generate variants of proteins that have acquired new characteristics.
Didier Saboulard, Vincent Dugas, Mehdi Jaber, Jérôme Broutin, Eliane Souteyrand, Julien Sylvestre, Marc Delcourt   +6 more
doaj   +1 more source

Different Approaches For Protein Engineering In Industrial Biotechnology [PDF]

, 2011
Protein engineering is the novel field which has wide applications from pharmaceutics, industry, commercial, laundry and research. It may apply rational design or non rational design or both.
Israr Khan, Muhammad Waheed Akhtar
core   +1 more source

Involvement of BcYak1 in the Regulation of Vegetative Differentiation and Adaptation to Oxidative Stress of Botrytis cinerea

Frontiers in Microbiology, 2018
Yak1, a member of the dual-specificity tyrosine phosphorylation-regulated protein kinases, plays an important role in diverse cellular processes in fungi. However, to date, the role of BcYak1 in Botrytis cinerea, the causal agent of gray mold diseases in
Qianqian Yang, Jianan Zhang, Jicheng Hu, Xue Wang, Binna Lv, Wenxing Liang   +5 more
doaj   +1 more source