An efficient one-step site-directed and site-saturation mutagenesis protocol [PDF]
, 2017 We have developed a new primer design method based on the QuickChange™ site-directed mutagenesis protocol, which significantly improves the PCR amplification efficiency.
Baumann, Ulrich +2 more
core
Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027 [PDF]
, 2016 Comparative analysis of the enediyne biosynthetic gene clusters revealed sets of conserved genes serving as outstanding candidates for the enediyne core.
Babnigg, Gyorgy +17 more
core +2 more sources
Journal of Lipid Research, 2019 Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes LDL receptor (LDLR) degradation, increasing plasma levels of LDL cholesterol and the risk of cardiovascular disease.
Shi-jun Deng +5 more
doaj +1 more source
BioTechniques, 1999 Site-directed mutagenesis is a powerful tool that has enabled molecular biologists to perform functional analysis of altered nucleic acids and proteins.
Alexander Turchin, Joseph F. Lawler
doaj +1 more source
Human carboxylesterase 2: Studies on the role of glycosylation for enzymatic activity
Biochemistry and Biophysics Reports, 2016 Human carboxylesterase 2 (hCES2) is a glycoprotein involved in the metabolism of drugs and several environmental xenobiotics, whose crystallization has been proved to be a challenging task.
Márcia Alves +7 more
doaj +1 more source
Broad Purpose Vector for Site-Directed Insertional Mutagenesis in Bifidobacterium breve
Frontiers in Microbiology, 2021 Members of the genus Bifidobacterium are notoriously recalcitrant to genetic manipulation due to their extensive and variable repertoire of Restriction-Modification (R-M) systems.
Emily C. Hoedt +14 more
doaj +1 more source
Site directed mutagenesis of L-pyrrolysine in dimethylamine methyltransferase [PDF]
, 2009 Methanosarcina barkeri is an archaeon and is able to use methylamines as growth substrates for methanogenesis. The methylamine methyltransferases that are involved in initiating methanogenesis from the methylamines have an in-frame amber codon (UAG) that
Patel, Jigar
core
Structural modeling and functional analysis of the essential ribosomal processing protease Prp from Staphylococcus aureus [PDF]
, 2017 In Firmicutes and related bacteria, ribosomal large subunit protein L27 is encoded with a conserved N-terminal extension that is removed to expose residues critical for ribosome function. Bacteria encoding L27 with this N-terminal extension also encode a
Christie, Gail E. +3 more
core +2 more sources
High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach
Bio-Protocol, 2020 Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is
Franziska Heydenreich +3 more
doaj +1 more source
Efficient method for site-directed mutagenesis in large plasmids without subcloning. [PDF]
PLoS ONE, 2017 Commonly used methods for site-directed DNA mutagenesis require copying the entire target plasmid. These methods allow relatively easy modification of DNA sequences in small plasmids but become less efficient and faithful for large plasmids ...
Louay K Hallak +5 more
doaj +1 more source