Site-directed mutagenesis of the proposed catalytic amino acids of the Sindbis virus capsid protein autoprotease [PDF]
, 1990 The structural proteins of Sindbis virus are translated as a polyprotein precursor that is cleaved upon translation. The capsid protein is postulated to be a serine protease that releases itself from the N terminus of the nascent polyprotein by ...
Hahn, Chang S., Strauss, James H.
core
Consensus mutagenesis reveals that non-helical regions influence thermal stability of horseradish peroxidase [PDF]
, 2008 The enzyme horseradish peroxidase has many uses in biotechnology but a stabilized derivative would have even wider applicability. To enhance thermal stability, we applied consensus mutagenesis (used successfully with other proteins) to recombinant ...
Amin +56 more
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Journal of Pharmacological Sciences, 2007 Based on radio-ligand binding and molecular modeling studies, sarpogrelate shows a moderate selectivity for 5-HT2B versus 5-HT2A receptors. To confirm the modeling data of sarpogrelate to 5-HT2B receptors predicting interaction of sarpogrelate towards ...
Habib Abul Muntasir +6 more
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A single amino acid substitution in the alpha 3 domain of an H-2 class I molecule abrogates reactivity with CTL. [PDF]
, 1987 We previously described a somatic cell expressing a variant H-2Dd molecule that did not serve as a target for alloreactive anti-Dd CTL. The mutant cell line had been isolated by its failure to express a serological epitope present on the H-2Dd alpha 3 ...
Bluestone, JA, Potter, TA, Rajan, TV
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Converting CooA from a Carbon Monoxide to an Oxygen-Sensing Heme Protein Transcription Factor: Investigations into the Structure and Mechanism of Gas Binding [PDF]
, 2011 CooA is a carbon monoxide-sensing (CO-sensing) heme protein transcription factor that regulates gene activation in several bacteria and, importantly, is a convenient model for studying analogous proteins in the human body.
Sedoris, Amanda, Wagoner, Josh
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Plasmodium falciparum glyoxalase II: Theorell-Chance product inhibition patterns, rate-limiting substrate binding via Arg(257)/Lys(260), and unmasking of acid-base catalysis [PDF]
, 2009 Glyoxalase II (GloII) is a ubiquitous thioester hydrolase catalyzing the last step of the glutathione-dependent conversion of 2-oxoaldehydes to 2-hydroxycarboxylic acids.
Elia A.C. +6 more
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Study on increase of nicotinamide mononucleotide yield by site-directed mutagenesis of nicotinamide phosphoribosyltransferase [PDF]
Zhongguo niangzaoNicotinamide phosphoribosyltransferase (NAMPT) was a key rate-limiting enzyme in the biosynthetic pathway of nicotinamide mononucleotide (NMN). In this study, the NAMPT gene derived from Comamonadaceae bacterium was heterologous expressed in Escherichia ...
DONG Ping, XU Yifan, HOU Ying, LUO Xuegang, ZHANG Lin, QIAO Changsheng
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Flexible and rapid construction of viral chimeras applied to Hepatitis C Virus [PDF]
, 2016 A novel and broadly applicable strategy combining site directed mutagenesis and DNA assembly for constructing seamless viral chimeras is described using Hepatitis C Virus as an exemplar. Full-length HCV genomic cloning cassettes, which contained flexibly
Alexander W. Tarr +7 more
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Selection Strategy for Site-Directed Mutagenesis Based on Altered β-Lactamase Specificity
BioTechniques, 1998 Conventional approaches to oligonucleotide-directed mutagenesis rely upon the application of a selection strategy to maximize mutagenesis efficiencies.
Christine A. Andrews, Scott A. Lesley
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Multiple site-directed mutagenesis via simple cloning by prolonged overlap extension
BioTechniques, 2020 We describe the application of simple cloning by prolonged overlap extension for multiple site-directed mutagenesis in the same plasmid. We show that it is possible to use this technique with very short PCR templates.
Rasmus Hejlesen +1 more
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