Site-directed mutagenesis v1 [PDF]
, 2020 Site-directed mutagenesis (SDM) as described here is used to introduce a mutation in the sequence of a plasmid of interest by PCR-based methods. Several different approaches have been previously reported, and also the generation of multiple mutations in a single step is possible.
openaire +1 more source
Exploring Important Protein Interactions in the Mammalian Diaphanous-related Formins: A Closer Look at the Relationship between the Intracellular Skeletal Network and a Single Amino Acid Residue [PDF]
, 2006 The Diaphanous-related formin protein family plays a key role in intracellular cytoskeletal regulation. Due to their farreaching importance, these proteins must be highly regulated themselves.
Hehl, Brent
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High-Level Expression of Various Apolipoprotein (a) Isoforms by "Transferrinfection". The Role of Kringle IV Sequences in the Extracellular Association with Low-Density Lipoprotein [PDF]
, 1994 Characterization of the assembly of lipoprotein(a) [Lp(a)] is of fundamental importance to understanding the biosynthesis and metabolism of this atherogenic lipoprotein. Since no established cell lines exist that express Lp(a) or apolipoprotein(a) [apo(a)
Azrolan N. +45 more
core +1 more source
Mutagenic and Spectroscopic Investigation of pH Dependent CooA DNA Binding [PDF]
, 2017 The carbon monoxide (CO) sensing heme protein, CooA, is a transcription factor which exists in several bacteria that utilize CO as an energy source. CooA positively regulates the expression of coo genes in the presence of CO such that the corresponding ...
Weaver, Brian R.
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ALS mutant FUS proteins are recruited into stress granules in induced pluripotent stem cells- derived motoneurons [PDF]
, 2015 Patient-derived induced Pluripotent Stem Cells (iPSCs) provide an opportunity to study human diseases mainly in those cases where no suitable model systems are available.
Bozzoni, Irene +9 more
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Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperatures. [PDF]
, 2015 BackgroundTrichoderma reesei is a key cellulase source for economically saccharifying cellulosic biomass for the production of biofuels. Lignocellulose hydrolysis at temperatures above the optimum temperature of T. reesei cellulases (~50°C) could provide
Atreya, Meera E +7 more
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Antimicrobial characterization of site-directed mutagenesis of porcine beta defensin 2.
PLoS ONE, 2015 Porcine β defensin 2 (pBD2) is a small, cationic and amphiphilic antimicrobial peptide. It has broad antimicrobial activities against bacteria and plays an important role in host defense.
Xian-xian Huang +3 more
doaj +1 more source
Construction of a "mutagenesis cartridge" for poliovirus genome-linked viral protein: Isolation and characterization of viable and nonviable mutants [PDF]
, 1988 By following a strategy of genetic analysis of poliovirus, we have constructed a synthetic "mutagenesis cartridge" spanning the genome-linked viral protein coding region and flanking cleavage sites in an infectious cDNA clone of the type 1 (Mahoney ...
Dunn, John J. +5 more
core
Engineering of microheterogeneity-resistant p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens [PDF]
, 1914 By site-directed mutagenesis, Cys-116 was converted to Ser-116 in p-hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas fluorescens. In contrast to wild-type enzyme, the C116S mutant is no longer susceptible to oxidation by hydrogen peroxide and ...
Klaus Eschrich +23 more
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Scarless and site-directed mutagenesis in
BMC Biotechnology, 2007 Background A variety of techniques have been described which introduce scarless, site-specific chromosomal mutations. These techniques can be applied to make point mutations or gene deletions as well as insert heterologous DNA into bacterial vectors for ...
Berghman Luc R +7 more
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