Results 81 to 90 of about 347,258 (205)

Machine learning-guided directed evolution for protein engineering [PDF]

, 2019
Machine learning (ML)-guided directed evolution is a new paradigm for biological design that enables optimization of complex functions. ML methods use data to predict how sequence maps to function without requiring a detailed model of the underlying ...
Arnold, Frances H., Wu, Zachary, Yang, Kevin K.   +2 more
core   +1 more source

キイロショウジョウバエ由来のチオレドキシン還元酵素のC未端テトラペプチド配列は、ヒト肺由来のチオレドキシン還元酵素では酸化還元活性を示さない [PDF]

, 2007
The isozymes of mammalian thioredoxin reductase (TrxR) contain the penultimate selenocysteineresidue (SeCys) in the redox-active C-terminal tetrapeptide, -Gly-Cys-SeCys-Gly (end). Amutant form of the mammalian enzyme TrxR-X498C in which SeCys is replaced
Inagaki, Kenji, Kawamura, Kentaro, Kuwahara, Mitsuhiko, Tamura, Takashi   +3 more
core  

site-directed mutagenesis

Citation: 'site-directed mutagenesis' in the IUPAC Compendium of Chemical Terminology, 5th ed.; International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.12726 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms ...
openaire   +1 more source

Robust SH2 binding affinity indicates minimal SH3-to-SH2 communication in Grb2

Biology Direct
Growth factor receptor–bound protein 2 (Grb2) is a modular adaptor that links activated receptor tyrosine kinases to downstream signaling pathways through its SH3–SH2–SH3 architecture.
Eduarda Santos Ventura, Mariana Di Felice, Julian Toso, Valeria Pennacchietti, Angelo Toto, Stefano Gianni   +5 more
doaj   +1 more source

Allosteric modulation of Grb2 drives ligand-dependent signal responses

Biology Direct
Adaptor proteins play a crucial role in signal transduction by facilitating the assembly of protein complexes at specific subcellular domains. These multifunctional molecules contain multiple binding modules that enhance the efficiency and flexibility of
Mariana Di Felice, Lucrezia Romana Rolfi, Julian Toso, Valeria Pennacchietti, Eduarda S. Ventura, Angelo Toto, Antonella Tramutola, Stefano Gianni   +7 more
doaj   +1 more source

Mutations that Separate the Functions of the Proofreading Subunit of the Escherichia coli Replicase

, 2015
The dnaQ gene of Escherichia coli encodes the Ɛ subunit of DNA polymerase III, which provides the 3\u27 - 5\u27 exonuclease proofreading activity of the replicative polymerase.
Kreuzer, Kenneth N., Whatley, Zakiya
core   +1 more source

Site-Directed Mutagenesis v1

, 2018

Moriah Beck, Abby Jurgensmeier
openaire   +1 more source

Kinetic and Structural Analysis of the Mg2+ -binding Site of the Guanine Nucleotide-binding Protein p21 H-ras. [PDF]

, 1993
The coordination and binding of the Mg2+ ion in the nucleotide−binding site of p21 have been investigated using site−directed mutagenesis, kinetic methods, and phosphorous NMR.
Borasio, Gian Domenico, Goody, R. S., John, J., Rensland, H., Schlichting, I., Vetter, I., Wittinghofer, A.   +6 more
core  

Modification of a PCRBased Site-Directed Mutagenesis Method

BioTechniques, 1997
Constance L. Fisher, Guo Kui Pei
doaj   +1 more source

PCR-Generated Crossover Linkers for Site-Directed Mutagenesis

BioTechniques, 1997
A. Christopher Boyd, David J. Porteous
doaj   +1 more source