Results 1 to 10 of about 1,059,795 (177)
Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination. [PDF]
PLoS Genetics, 2013 Phage-encoded serine integrases mediate directionally regulated site-specific recombination between short attP and attB DNA sites without host factor requirements.
Shweta Singh +2 more
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High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells. [PDF]
PLoS ONE, 2007 DNA site-specific recombinases (SSRs) such as Cre, FLPe, and phiC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities,
Christopher S Raymond, Philippe Soriano
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Predicting algorithm of attC site based on combination optimization strategy
Connection Science, 2022 Site-specific recombination systems are widely used as bioengineering tools. However, the traditional site-specific recombination system requires a consensus sequence for the specific site.
Zhendong Liu +9 more
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Nature, 1981 Site-specific recombination is a reaction in which a pair of genetically defined sites undergoes reciprocal exchange (“crossing-over”) via a recombinase-mediated DNA breakage and joining process. Such reactions have a wide range of biological outcomes, from integration and excision of virus genomes into and out of host chromosomes, to acquisition of ...
David Sherratt, Avril Arthur, Paul Dyson
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BioTechniques, 2011 Site-specific recombination is a powerful biotechnological tool for genome engineering. We previously reported two novel site-specific recombination systems, VCre/VloxP and SCre/SloxP, that do not cross-react with Cre/loxP and Flp/FRT in culture cells ...
Sachiko Minorikawa, Manabu Nakayama
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Novel Heterotypic Rox Sites for Combinatorial Dre Recombination Strategies
G3: Genes, Genomes, Genetics, 2016 Site-specific recombinases (SSRs) such as Cre are widely used in gene targeting and genetic approaches for cell labeling and manipulation. They mediate DNA strand exchange between two DNA molecules at dedicated recognition sites. Precise understanding of
Katherine Chuang +3 more
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A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector. [PDF]
PLoS ONE, 2011 The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and animal transgenesis.
Shigeyuki Yamaguchi +5 more
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Recombination: Pieces of the site-specific recombination puzzle [PDF]
Current Biology, 1995 Understanding how nucleoprotein complexes interact with specific DNA sequences has come closer with structural and mechanistic studies of the interaction between the recombination enzyme resolvase and DNA.
Oram, M., Szczelkun, M.D., Halford, S.E.
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An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
BMC Biotechnology, 2017 Background Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable ...
Michael J. Bland +3 more
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Development of a novel in vitro assay for the evaluation of integron DNA integrase activity
Biotechnology & Biotechnological Equipment, 2016 Integrons play an important role in multidrug resistance. The integron platform codes for integrase (intI) that is required for gene cassette integration through site-specific recombination.
Fatemeh Tohidi +6 more
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