Artificial nondirectional site-specific recombination systems [PDF]
iScience, 2022 Summary: Site-specific recombination systems (SRSs) are widely used in studies on synthetic biology and related disciplines. Nondirectional SRSs can randomly trigger excision, integration, reversal, and translocation, which are effective tools to achieve
Jun-Yi Wang +9 more
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Genome-wide detection of conservative site-specific recombination in bacteria. [PDF]
PLoS Genetics, 2018 The ability of clonal bacterial populations to generate genomic and phenotypic heterogeneity is thought to be of great importance for many commensal and pathogenic bacteria. One common mechanism contributing to diversity formation relies on the inversion
Ognjen Sekulovic +5 more
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phiC31 integrase-mediated site-specific recombination in barley. [PDF]
PLoS ONE, 2012 The Streptomyces phage phiC31 integrase was tested for its feasibility in excising transgenes from the barley genome through site-specific recombination.
Eszter Kapusi +4 more
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The λ Integrase Site-specific Recombination Pathway. [PDF]
Microbiol Spectr, 2015 ABSTRACTThe site-specific recombinase encoded by bacteriophage λ (Int) is responsible for integrating and excising the viral chromosome into and out of the chromosome of itsEscherichia colihost. Int carries out a reaction that is highly directional, tightly regulated, and depends upon an ensemble of accessory DNA bending proteins acting on 240 bp of ...
Landy A.
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FLP recombinase-mediated site-specific recombination in silkworm, Bombyx mori. [PDF]
PLoS ONE, 2012 A comprehensive understanding of gene function and the production of site-specific genetically modified mutants are two major goals of genetic engineering in the post-genomic era.
Ding-Pei Long +7 more
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Model-guided engineering of DNA sequences with predictable site-specific recombination rates [PDF]
Nature Communications, 2022 Site-specific recombination (SSR) is an important tool in synthetic biology, but its applications are limited by the inability to predictably tune SSR reaction rates.
Qiuge Zhang +2 more
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The Site-Specific Recombination System of the Escherichia coli Bacteriophage Φ24B [PDF]
Frontiers in Microbiology, 2020 Stx bacteriophages are members of the lambdoid group of phages and are responsible for Shiga toxin (Stx) production and the dissemination of Shiga toxin genes (stx) across shigatoxigenic Escherichia coli (STEC).
Mohammed Radhi Mohaisen +4 more
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Compatibility of Site-Specific Recombination Units between Mobile Genetic Elements [PDF]
iScience, 2020 Summary: Site-specific recombination (SSR) systems are employed for transfer of mobile genetic elements (MGEs), such as lysogenic phages and integrative conjugative elements (ICEs).
Shota Suzuki +5 more
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Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli [PDF]
Journal of Biological EngineeringBackground Site-specific recombination (SSR) systems are essential tools for conditional genetic manipulation and are valued for their efficacy and user friendliness. However, the development of novel SSR strategies is urgently needed.
Chichu Xie +8 more
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Bacteriophage lambda site-specific recombination. [PDF]
Mol MicrobiolAbstractThe site‐specific recombination pathway of bacteriophage λ encompasses isoenergetic but highly directional and tightly regulated integrative and excisive reactions that integrate and excise the vial chromosome into and out of the bacterial chromosome.
Van Duyne GD, Landy A.
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