The integrase of genomic island GIsul2 mediates the mobilization of GIsul2 and ISCR-related element CR2-sul2 unit through site-specific recombination [PDF]
Frontiers in Microbiology, 2022 In the worldwide health threat posed by antibiotic-resistant bacterial pathogens, mobile genetic elements (MGEs) play a critical role in favoring the dissemination of resistance genes.
Gang Zhang +14 more
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Large T antigen mediated target gene replication improves site-specific recombination efficiency [PDF]
Frontiers in Bioengineering and BiotechnologyWith advantages of high-fidelity, monoclonality and large cargo capacity, site-specific recombination (SSR) holds great promises for precise genomic modifications.
Zening Wang +4 more
doaj +2 more sources
Temporally-controlled site-specific recombination in zebrafish. [PDF]
PLoS ONE, 2009 Conventional use of the site-specific recombinase Cre is a powerful technology in mouse, but almost absent in other vertebrate model organisms. In zebrafish, Cre-mediated recombination efficiency was previously very low.
Stefan Hans +4 more
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Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis [PDF]
BMC Biotechnology, 2019 Background Site-specific integration system allows foreign DNA to be integrated into the specific site of the host genome, enabling stable expression of heterologous protein.
Innanurdiani Koko +3 more
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Pandemic Vibrio cholerae shuts down site-specific recombination to retain an interbacterial defence mechanism [PDF]
Nature Communications, 2020 Vibrio cholerae uses a type VI secretion system (T6SS) to kill neighbouring competitors. Here, Santoriello et al. show that a T6SS gene cluster (Aux3) exists as a mobile, prophage-like element in some environmental strains, and as a stable truncated form
Francis J. Santoriello +3 more
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Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination. [PDF]
PLoS ONE, 2013 Streptomyces phage φBT1 integrates its genome into the attB site of the host chromosome with the attP site to generate attL and attR. The φBT1 integrase belongs to the large serine recombinase subfamily which directly binds to target sites to initiate ...
Lin Zhang +4 more
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Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts [PDF]
Nature CommunicationsSite-specific recombinases such as the Cre-LoxP system are routinely used for genome engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the CRISPR-Cas toolbox and provide the additional benefit of high-efficiency DNA ...
Charlotte Cautereels +9 more
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Dynamics in Cre-loxP site-specific recombination. [PDF]
Curr Opin Struct BiolCre recombinase is a phage-derived enzyme that has found utility for precise manipulation of DNA sequences. Cre recognizes and recombines pairs of loxP sequences characterized by an inverted repeat and asymmetric spacer. Cre cleaves and religates its DNA targets such that error-prone repair pathways are not required to generate intact DNA products ...
Foster MP +3 more
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Strategies for site-specific recombination with high efficiency and precise spatiotemporal resolution. [PDF]
J Biol Chem, 2021 Site-specific recombinases (SSRs) are invaluable genome engineering tools that have enormously boosted our understanding of gene functions and cell lineage relationships in developmental biology, stem cell biology, regenerative medicine, and multiple ...
Tian X, Zhou B.
europepmc +2 more sources
Application of the Cre/loxP Site-Specific Recombination System for Gene Transformation in Aurantiochytrium limacinum [PDF]
Molecules, 2015 The Cre/loxP site-specific recombination system was applied to Aurantiochytrium limacinum to obtain a transformant without the antibiotic resistance marker gene.
Hengyi Sun +8 more
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