Results 51 to 60 of about 1,059,795 (177)
Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system
Nucleic Acids Research, 2012 Targeted genome engineering has become an important research area for diverse disciplines, with site-specific recombinases (SSRs) being among the most popular genome engineering tools.
Madina Karimova +5 more
semanticscholar +1 more source
Efficient Cre/loxP Site-Specific Recombination in a HepG2 Human Liver Cell Line
Cell Transplantation, 2000 A worldwide shortage of donor livers is a limiting factor of the clinical application of hepatocyte transplantation (HTX). To resolve this issue, we focused on a reversible immortalization system that allows temporary expansion of primary hepatocyte ...
Naoya Kobayashi M.D., Ph.D. +8 more
doaj +1 more source
Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells
BMC Biotechnology, 2004 Background Cell-permeant Cre DNA site-specific recombinases provide an easily controlled means to regulate gene structure and function in living cells. Since recombination provides a stable and unambiguous record of protein uptake, the enzyme may also be
Ruley H Earl +3 more
doaj +1 more source
Integrase-mediated recombination of the veb1 gene cassette encoding an extended-spectrum β-lactamase. [PDF]
PLoS ONE, 2012 The veb1 gene cassette encodes the extended spectrum β-lactamase, VEB-1 that is increasingly isolated from worldwide Gram-negative rods. Veb1 is commonly inserted into the variable region of different class 1 integrons in which it is always associated ...
Daniel Aubert +2 more
doaj +1 more source
Single-Molecule Tethered Particle Motion: Stepwise Analyses of Site-Specific DNA Recombination
Micromachines, 2018 Tethered particle motion/microscopy (TPM) is a biophysical tool used to analyze changes in the effective length of a polymer, tethered at one end, under changing conditions.
Hsiu-Fang Fan +2 more
doaj +1 more source
Nucleic Acids Research, 2012 Tyrosine family recombinases (YRs) are widely utilized in genome engineering systems because they can easily direct DNA rearrangement. Cre recombinases, one of the most commonly used types of YRs, catalyze site-specific recombination between two loxP ...
Hsiu‐Fang Fan
semanticscholar +1 more source
mBio, 2017 Integrons ensure a rapid and “on demand” response to environmental stresses driving bacterial adaptation. They are able to capture, store, and reorder functional gene cassettes due to site-specific recombination catalyzed by their integrase.
Céline Loot +7 more
doaj +1 more source
Topological Selectivity in Xer Site-Specific Recombination [PDF]
Cell, 1997 The product topology of Xer-mediated site-specific recombination at plasmid sites has been determined. The product of deletion at pSC101 psi is a right-handed antiparallel 4-noded catenane. The ColE1 cer deletion product has an identical topology, except that only one pair of strands is exchanged.
Colloms, S, Bath, J, Sherratt, D
openaire +3 more sources
The unconventional Xer recombination machinery of Streptococci/Lactococci.
PLoS Genetics, 2007 Homologous recombination between circular sister chromosomes during DNA replication in bacteria can generate chromosome dimers that must be resolved into monomers prior to cell division.
Pascal Le Bourgeois +8 more
doaj +1 more source
Microcin H47 system: an Escherichia coli small genomic island with novel features. [PDF]
PLoS ONE, 2011 Genomic islands are DNA regions containing variable genetic information related to secondary metabolism. Frequently, they have the ability to excise from and integrate into replicons through site-specific recombination.
María F Azpiroz +2 more
doaj +1 more source