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Expression of cystatin C in the female reproductive tract and its effect on human sperm capacitation
Reproductive Biology and Endocrinology, 2018 Background Cystatin C (CST3), a cysteine protease inhibitor in seminal plasma, is expressed in animal uteri. However, its expression in the human female reproductive tract and its effect on human sperm capacitation are unclear.
Robert Kuo-Kuang Lee +7 more
doaj +1 more source
, 2012 The ejaculated semen consists of two major components viz. sperm cells (spermatozoa) and the fluid part obtained after centrifugation called seminal plasma.
P Perumal
core +2 more sources
Molecular Basis of Human Sperm Capacitation [PDF]
Frontiers in Cell and Developmental Biology, 2018 In the early 1950s, Austin and Chang independently described the changes that are required for the sperm to fertilize oocytes in vivo. These changes were originally grouped under name of "capacitation" and were the first step in the development of in vitro fertilization (IVF) in humans.
Puga Molina, Lis del Carmen +5 more
openaire +4 more sources
Effect of Astaxanthin on Human Sperm Capacitation [PDF]
Marine Drugs, 2013 In order to be able to fertilize oocytes, human sperm must undergo a series of morphological and structural alterations, known as capacitation. It has been shown that the production of endogenous sperm reactive oxygen species (ROS) plays a key role in causing cells to undergo a massive acrosome reaction (AR).
DONA', GABRIELLA +9 more
openaire +4 more sources
Reorganization of mouse sperm lipid rafts by capacitation [PDF]
, 2006 One of the hallmarks of mammalian sperm capacitation is the loss of cholesterol from the plasma membrane. Cholesterol has been associated with the formation of detergent insoluble membrane microdomains in many cell types, and sperm from several mammalian
Ramalie, Jenniffer R. +2 more
core +1 more source
Clinically relevant enhancement of human sperm motility using compounds with reported phosphodiesterase inhibitor activity [PDF]
, 2014 STUDY QUESTION: Can we identify compound(s) with reported phosphodiesterase inhibitor (PDEI) activity that could be added to human spermatozoa in vitro to enhance their motility without compromising other sperm functions?
Alasmari +54 more
core +5 more sources
Specific lectin binding sites during in vitro capacitation and acrosome reaction in boar spermatozoa
Italian Journal of Animal Science, 2021 Sperm glycocalyx and plasma membrane undergo outstanding modifications during fertilization. However, it is unclear how in vitro capacitation time and acrosome reaction affect the specific location of boar sperm glycoconjugates.
Laura Robles-Gómez +6 more
doaj +1 more source
Biology, 2021 This work sought to address whether the presence of exogenous bicarbonate is required for pig sperm to elicit in vitro capacitation and further progesterone-induced acrosome exocytosis. For this purpose, sperm were either incubated in a standard in vitro
Bruna Resende Chaves +7 more
doaj +1 more source
STUDIES ON SPERM CAPACITATION [PDF]
Reproduction, 1973 Summary. During incubation of guinea-pig spermatozoa in Ca-free Krebs—Ringer phosphate buffer, substances were released into the medium which were subsequently separated on Sephadex G-200 into two major fractions, Fr. I and Fr. II. The macromolecular Fr. I increased with sperm maturation and the fraction was shown to
S. AONUMA +5 more
openaire +1 more source
Membrane Potential Assessment by Fluorimetry as a Predictor Tool of Human Sperm Fertilizing Capacity
Frontiers in Cell and Developmental Biology, 2020 Mammalian sperm acquire the ability to fertilize eggs by undergoing a process known as capacitation. Capacitation is triggered as the sperm travels through the female reproductive tract.
Carolina Baro Graf +8 more
doaj +1 more source