Dorsomorphin promotes survival and germline competence of zebrafish spermatogonial stem cells in culture. [PDF]
PLoS ONE, 2013 Zebrafish spermatogonial cell cultures were established from Tg(piwil1:neo);Tg(piwil1:DsRed) transgenic fish using a zebrafish ovarian feeder cell line (OFC3) that was engineered to express zebrafish Lif, Fgf2 and Gdnf.
Ten-Tsao Wong, Paul Collodi
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Establishment of cell lines with rat spermatogonial stem cell characteristics [PDF]
, 2002 Spermatogonial cell lines were established by transfecting a mixed population of purified rat A(s) (stem cells), A(pr) and A(al) spermatogonia with SV40 large T antigen.
van Pelt, Ans M. M. +5 more
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Sterile testis complementation with spermatogonial lines restores fertility to DAZL-deficient rats and maximizes donor germline transmission. [PDF]
PLoS ONE, 2009 Despite remarkable advances in assisted reproductive capabilities approximately 4% of all couples remain involuntarily infertile. In almost half of these cases, a lack of conception can in some measure be attributed to the male partner, wherein de novo Y-
Timothy E Richardson +4 more
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Plasticity of spermatogonial stem cells
Asian Journal of Andrology, 2015 There have been significant breakthroughs over the past decade in the development and use of pluripotent stem cells as a potential source of cells for applications in regenerative medicine. It is likely that this methodology will begin to play an important role in human clinical medicine in the years to come. This review describes the plasticity of one
Paul S Cooke +4 more
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Regulation of the Spermatogonial Stem Cell Niche [PDF]
Reproduction in Domestic Animals, 2008 ContentsSpermatogonial stem cells (SSCs) reside within specialized microenvironments called ‘niches’, which are essential for their maintenance and self‐renewal. In the mammalian testis, the main components of the niche include the Sertoli cell, the growth factors that this nursing cell produces, the basement membrane, and stimuli from the vascular ...
N, Kostereva, M-C, Hofmann
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Vaccinia-related kinase 1 is required for the maintenance of undifferentiated spermatogonia in mouse male germ cells. [PDF]
PLoS ONE, 2010 Vaccinia-related kinase 1 (VRK1) is a crucial protein kinase for mitotic regulation. VRK1 is known to play a role in germ cell development, and its deficiency results in sterility.
Yoon Ha Choi +9 more
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Regulation of Spermatogonial Stem Cell Self-Renewal in Mammals [PDF]
, 2008 Mammalian spermatogenesis is a classic adult stem cell–dependent process, supported by self-renewal and differentiation of spermatogonial stem cells (SSCs).
Brinster, Ralph L, Oatley, Jon M
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The Neonatal and Adult Human Testis Defined at the Single-Cell Level
Cell Reports, 2019 Summary: Spermatogenesis has been intensely studied in rodents but remains poorly understood in humans. Here, we used single-cell RNA sequencing to analyze human testes.
Abhishek Sohni +10 more
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Three-Dimensional Culture of Mouse Spermatogonial Stem Cells Using A Decellularised Testicular Scaffold [PDF]
Cell Journal, 2019 Objective: Applications of biological scaffolds for regenerative medicine are increasing. Such scaffolds improve cell attachment, migration, proliferation and differentiation. In the current study decellularised mouse whole testis was used as a natural
Nasrin Majidi Gharenaz +2 more
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FOXP4 promotes proliferation of human spermatogonial stem cells
Asian Journal of Andrology, 2023 Continuous self-renewal and differentiation of spermatogonial stem cells (SSCs) is vital for maintenance of adult spermatogenesis. Although several spermatogonial stem cell regulators have been extensively investigated in rodents, regulatory mechanisms ...
Shu-Wei Luo +4 more
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