Results 71 to 80 of about 117,630 (212)

Hydrogel microenvironments for cancer spheroid growth and drug screening

Science Advances, 2018
Man-made biomimetic hydrogels with tunable biophysical and biochemical properties can support the growth of microtumors. Multicellular cancer spheroids (MCSs) have emerged as a promising in vitro model that replicates many features of solid tumors in ...
Yunfeng Li, E. Kumacheva
semanticscholar   +1 more source

Assembly of Multi‐Spheroid Cellular Architectures by Programmable Droplet Merging

Advances in Materials, 2020
Artificial multicellular systems are gaining importance in the field of tissue engineering and regenerative medicine. Reconstruction of complex tissue architectures in vitro is nevertheless challenging, and methods permitting controllable and high ...
Haijun Cui, Xianxian Wang, Janine Wesslowski, T. Tronser, Jakob Rosenbauer, A. Schug, G. Davidson, A. Popova, P. Levkin   +8 more
semanticscholar   +1 more source

SpheroMold: modernizing the hanging drop method for spheroid culture

Frontiers in Drug Delivery
The hanging drop method is a cost-effective approach for 3D spheroid culture. However, obtaining numerous spheroids in a limited area becomes challenging due to the risk of droplet coalescence, primarly during Petri dish handling.
Ana Paula Pereira Guimaraes, Italo Rodrigo Calori, Italo Rodrigo Calori, Hong Bi, Antonio Claudio Tedesco, Antonio Claudio Tedesco   +5 more
doaj   +1 more source

AMPK Knockout Impairs the Formation of Three-Dimensional Spheroids

Life
AMP-activated protein kinase (AMPK) is an important regulator of cellular energy homeostasis, and AMPK contributes to cell growth, apoptosis, and autophagy.
Yea-In Park, Rackhyun Park, Siyun Lee, Chunghyeon Lee, Inkyu Yoo, Hakhyun Ka, Yang Hoon Huh, Jongkwang Hong, Junsoo Park   +8 more
doaj   +1 more source

Prediction of Drug-Induced Hepatotoxicity Using Long-Term Stable Primary Hepatic 3D Spheroid Cultures in Chemically Defined Conditions

Toxicological Sciences, 2018
High failure rates of drug candidates in the clinics, restricted-use warnings as well as withdrawals of drugs in postmarketing stages are of substantial concern for the pharmaceutical industry and drug-induced liver injury (DILI) constitutes one of the ...
Sabine U. Vorrink, Yitian Zhou, M. Ingelman-Sundberg, V. M. Lauschke   +3 more
semanticscholar   +1 more source

SpheroidJ: An Open-Source Set of Tools for Spheroid Segmentation

Comput. Methods Programs Biomed., 2020
BACKGROUND AND OBJECTIVES Spheroids are the most widely used 3D models for studying the effects of different micro-environmental characteristics on tumour behaviour, and for testing different preclinical and clinical treatments.
David Lacalle, Héctor Castro-Abril, T. Randelovic, C. Domínguez, Jónathan Heras, Eloy J. Mata, Gadea Mata, Yolanda Méndez, Vico Pascual, Ignacio Ochoa   +9 more
semanticscholar   +1 more source

Combined automated culture system for tubular structure assembly and maturation for vascular tissue engineering

Journal of Biomechanical Science and Engineering, 2018
Development of the robust yet practical tissue culture systems for vascular grafting is one of the major challenges for biomedical engineering. The following questions should be solved in priority: (1) flexibility of the tissue reservoir allowing dynamic
Kazutomo BABA, Andrey MIKHAILOV, Yoshiyuki SANKAI   +2 more
doaj   +1 more source

Human Cortex Spheroid with a Functional Blood Brain Barrier for High-Throughput Neurotoxicity Screening and Disease Modeling

Scientific Reports, 2018
The integral selectivity characteristic of the blood brain barrier (BBB) limits therapeutic options for many neurologic diseases and disorders. Currently, very little is known about the mechanisms that govern the dynamic nature of the BBB. Recent reports
Goodwell Nzou, R. Wicks, R. Wicks, Elizabeth E. Wicks, S. Seale, C. H. Sane, A. Chen, S. Murphy, John D. Jackson, A. Atala   +9 more
semanticscholar   +1 more source

Genetically-engineered human cortical spheroid models of Tuberous Sclerosis

Nature Medicine, 2018
Tuberous sclerosis complex (TSC) is a multisystem developmental disorder caused by mutations in the TSC1 or TSC2 genes, whose protein products are negative regulators of mechanistic target of rapamycin complex 1 signaling. Hallmark pathologies of TSC are
J. Blair, D. Hockemeyer, Helen S. Bateup
semanticscholar   +1 more source

A Chemically Defined Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation

Stem Cells International, 2020
Mesenchymal stem cells (MSCs) possess promising potential in tissue engineering and regenerative medicine. Previous studies demonstrated that spheroid formation of MSCs exhibited improved stemness maintenance and therapeutic potential compared with ...
Yajun Zhao, E. Xiao, Wanqi Lv, Xian Dong, Linhai He, Yixiang Wang, Yi Zhang   +6 more
doaj   +1 more source